SARS coronavirus protein 7a interacts with human Ap 4 A-hydrolaseResearch Published on 2010-02-092022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] activated Activation Amino acid Apoptosis cell proliferation cellular co-immunoprecipitation culture cell cytoplasm DNA repair DNA replication ENCODE expressing Frame Host Human human cell interact Interaction involved nucleotide ORF pathogenic processe Protein protein synthesis Proteins Regulation responsible RNA SARS Coronavirus SARS-CoV sequence homology shown tissue virus yeast [DOI] 10.1186/1743-422X-7-31 PMC 바로가기 [Article Type] Research
Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane proteinResearch Published on 2010-01-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] accessory protein addition Alpha Amino acid Analysis antiserum C-terminus Cell conserved coronavirus Coronaviruses cytosolic detected digestion ENCODE ERGIC Evidence expression Extracellular FLAG-tagged fluorescence microscopy Frame function Golgi Huh-7 cell Human human coronavirus immunofluorescence In-vitro Infected lysosomal membrane membrane protein N-terminus NL63 nucleocapsid Occurrence ORF pathogenic coronavirus predicted Protein purified reading Respiratory tract infections Result sequence shown structural protein subsequent Topology translation viral particle virion [DOI] 10.1186/1743-422X-7-6 PMC 바로가기 [Article Type] Research
Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivoResearch Published on 2009-08-242022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] Bacterial caused cDNA Cell Cell culture cells changes in chromosome cleavage cloned compared contributed deleted downstream early phase explain Frame full-length protein Genetic Genome genomic hamster identify In-vitro induction of apoptosis Infection Infectious virus inoculated interferon beta intranasally lung observation ORF outbreak over-expressed parental Patient plaque forming unit promoter Protein prototype virus reconstructed replicate replicative reveal RNA SARS-CoV secretion Spread strain Strains suggested supernatant T7 RNA polymerase the SARS epidemic transcribed transfection transmembrane domain type I interferon variant Vero cells Viral RNA virulence virus virus isolate virus replication virus RNA [DOI] 10.1186/1743-422X-6-131 PMC 바로가기 [Article Type] Research
Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks ( Anas platyrhynchos )Article Published on 2009-05-042022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] Ct, threshold cycle amplified Ana avian coronavirus competing conserved Coronaviruses CoV CoVs decrease detect detection duck fatal disease fecal samples found Frame FRET, fluorescence resonance energy transfer groups host range Human LNA, locked nucleic acid NQPCR, nuclease-based real-time reverse transcription PCR Nuclease-based probes ORF, open reading frame PCR, polymerase chain reaction Primers probe probes provide QPCR, quantitative real-time reverse transcription PCR Real-time PCR reduced reservoirs respiratory sample reverse transcription PCR RNA, Ribonucleic acid RT-PCR, reverse transcriptase polymerase chain reaction sensitivity sequence Split probe strategy Spread Strains TaqMan targets TD, touch down temperature protocol TET, tetrachloro-6-carboxyfluorescein Transmission variant viral evolution viral nucleic acid [DOI] 10.1016/j.jviromet.2009.04.022 PMC 바로가기 [Article Type] Article
SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epitheliumArticle Published on 2007-04-232022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] addition airway airway epithelial cells airway epithelium Antisera apical surface Atypical Calu-3 Calu-3 cell Cell cells cellular Characteristics ciliated ciliated cell consequence coronavirus coronavirus pathogenesis Coronavirus replication Culture cultures detected develop did not reduce dry cough efficient enzyme Epidemics epithelial epithelial cell epithelial cell line Experimental result feature Fever fluorescent Frame GFP hACE2 HAE Human human airway epithelia human airway epithelial Human airway epithelium human lung human pathogen in vitro in vitro model in vivo Infection inoculated insertion late stage log Lower respiratory tract lung lung epithelial mechanism Morphological new strain onset of symptoms ORF Pathogenesis pathology physiological Pneumonia Post-infection primary cell progeny virion Protein provide reached receptor reduced replicate replicated Replication reported reservoir respiratory virus resulting SARS Coronavirus SARS-CoV SARS-CoV GFP SARS-CoV infection Support susceptible target tight junction Trachea Vero E6 viral infection viral replication viral titer virus wild type zoonotic [DOI] 10.1016/j.virusres.2007.03.013 PMC 바로가기 [Article Type] Article
Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41Article Published on 2002-11-122022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Amino acid Analysis blocked bronchitis chymotrypsin cleavage cleavage site coronavirus coronavirus IBV determined Frame IBV identify in vivo infectious leucine M41 membrane N-terminal N-terminus nucleocapsid nucleotide sequence Partial peptide performed precursor propolypeptide protein sequencing related removed residue S1 and S2 sequence Sequencing Serine spike Spike protein strain trypsin Vero Cell Virus spike [DOI] 10.1016/0168-1702(86)90037-7 PMC 바로가기 [Article Type] Article