Discovery and characterization of SARS-CoV-2 reactive and neutralizing antibodies from humanized CAMouseHG mice through rapid hybridoma screening and high-throughput single-cell V(D)J sequencingArticle Published on 2022-09-232022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome antibodies antibody antibody humanized mice antibody repertoire Antibody Response assays can be used caused Cell clone complementary coronavirus coronavirus disease cynomolgus macaques death deficiency develop diagnostic effective Efficacy evade fully human antibody Genes humanized humans IGHV6-1 immune response immunization immunized Infection Measures medium mice MOST mutate Neutralizing neutralizing antibody pandemic patients with COVID-19 platform produced Prophylactic Protective reactive reactive and neutralizing antibodies Replication SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD binding SARS-CoV-2 virus Sequencing Sequencing analysis significantly single-cell single-cell sequencing the antibody therapeutic variant Virus neutralization [DOI] 10.3389/fimmu.2022.992787 PMC 바로가기
Immunogenicity of two COVID-19 vaccines used in India: An observational cohort study in health care workers from a tertiary care hospitalArticle Published on 2022-09-232022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, [키워드] adenoviral-vectored vaccine antibodies blood sample booster Care Cell collected Covaxin COVID-19 COVID-19 case COVID-19 vaccine Covishield diminished dose elicited ELISA full-length Health care worker hospital immune response Immunity immunization immunogenicity independent India initiated IQR long-term follow up median Neutralizing Neutralizing antibody titer Observational cohort study pandemic PBMC pre-vaccination progression recipient recipients remained SARS CoV-2 Seroconversion T cell T cell response T-cell Response tested Vaccine Vaccines were recorded were used [DOI] 10.3389/fimmu.2022.928501 PMC 바로가기
Neuroinflammation in Dementia-Therapeutic Directions in a COVID-19 Pandemic SettingArticle Published on 2022-09-222022-11-15 Journal: Cells [Category] SARS, 진단, [키워드] activate Activation Affect cognitive complications Concentration COVID-19 cytokine production Dementia direction dysfunction Efficacy Factors Glia glial cell glial cells group of diseases heterogenous highlight human organism IL-1β immune response Inconclusive Inflammation Inflammatory Intervention molecular neurodegenerative Neuroinflammation Neuron NSAID outcome pathology peripheral pro-inflammatory signaling provide resulting risk SARS-CoV-2 setting shown Side effects therapeutic approaches TNF TNF antagonist TNF antagonists while [DOI] 10.3390/cells11192959 PMC 바로가기
Intranasal immunization with recombinant Vaccinia virus encoding trimeric SARS-CoV-2 spike receptor-binding domain induces neutralizing antibodyArticle Published on 2022-09-222022-11-15 Journal: Vaccine [Category] SARS, 진단, 치료기술, [키워드] BALB/c mice Bronchoalveolar lavage fluid cellular immune responses COVID-19 detectable dissemination domain Effects elicited evaluated exhibited expressing humoral IgA IgG immune response immunization immunized induce Infection intranasal Lymphocytes mice mucosal mucosal immune nasal washe neutralization neutralization activities Neutralizing Neutralizing antibodies neutralizing antibody RBD respiratory response robust SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein secretory IgA sera systemic immune response transmission of SARS-CoV-2 trimeric trimeric RBD Vaccine vaccinia vaccinia virus virus [DOI] 10.1016/j.vaccine.2022.08.054 PMC 바로가기
Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated ResponseArticle Published on 2022-09-212022-11-15 Journal: Viruses [Category] SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] A549 cell acute phase assumed both virus clinical Course cytosolic cytosolic sensors detection disease onset Disease progression donors elevated evaluated expression Genes H3N2 immune response in vitro in vitro transcribed RNAs influenza A influenza A virus inhibit interferon-stimulated gene interferons ISGs MDA5 Mild-to-moderate mRNA expression mRNA level multiple mechanism multiplex not differ observation onset of symptoms pathogenesis of SARS-CoV-2 patients with influenza post infection qPCR qPCR assay remained response RNA RNAs SARS-CoV-2 SARS-CoV-2 patient Signaling Simultaneous SOCS-1 the disease transcribed transfected with type I virus White blood cell White blood cells worsening [DOI] 10.3390/v14102090 PMC 바로가기
Single-Cell Gene Expression Analysis Revealed Immune Cell Signatures of Delta COVID-19Article Published on 2022-09-212022-11-16 Journal: Cells [Category] SARS, 변종, 유전자 메커니즘, [키워드] accompanied accompany adaptive B.1.617 Blood caused Characteristics contribute coronavirus disease correlated COVID-19 COVID-19 pathogenesis Cytokine storm Delta delta variant diagnostic dominant Factor Gene Expression gene expression signature high mortality highlight immune immune component immune response immune system Immunity immunological individual Infection Lineage lymphoid molecular monocyte Monocytes mononuclear cell Multiple myeloid cells pathway performed proinflammatory required Revealed risk SARS-CoV-2 SARS-CoV-2 lineage severity signature significant changes in single-cell syndrome targeted therapy therapeutic Transcriptome understanding variant virus with COVID-19 [DOI] 10.3390/cells11192950 PMC 바로가기
Humoral immunoresponse elicited against an adenoviral-based SARS-CoV-2 coronavirus vaccine in elderly patientsArticle Published on 2022-09-212022-11-15 Journal: Aging (Albany NY) [Category] SARS, 진단, 치료기술, [키워드] Ad26 Ad5 analyzed anti-RBD anti-RBD ELISA anti-RBD IgG antibodies antibody Cohort COVID-19 detectable dose dropped effective vaccine elderly Elderly patient elicit elicited Evolution expected females Gam-COVID-Vac Gender Genome Heterologous highest humoral immune response IgG IgG titer immune response immune responses immune system individual median post vaccination reached remained robust SARS-CoV-2 SARS-CoV-2 coronavirus SARS-COV-2 infection Sequencing seroconversion rate significant decrease significant difference specific antibody the SARS-CoV-2 the Spike Vaccine viral genome virus Volunteer [DOI] 10.18632/aging.204299 PMC 바로가기
Long-term quantitative assessment of anti-SARS-CoV-2 spike protein immunogenicity (QUASI) after COVID-19 vaccination in older people living with HIV (PWH)Article Published on 2022-09-212022-11-15 Journal: BMC Infectious Diseases [Category] SARS, 진단, 치료기술, [키워드] age analyzed anti-SARS-CoV-2 Anti-spike Antiretroviral therapy black BNT162b2 booster CD4 CD8 cells clinically COVID-19 COVID-19 vaccination COVID-19 vaccine decrease demographics detectable dose exploratory female HIV Humoral immunity IgG IgG levels immune response immunogenicity majority male marker median MOST occurred offered Older outcome participant positive presenting primary analysis protective immunity quantitative assessment reactogenicity recruited responses SARS-CoV-2 secondary single-center Spike protein subject suppressed T-cell T-cell Response vaccination Viremia virus-specific T-cell response white Wilcoxon signed-rank test with HIV [DOI] 10.1186/s12879-022-07737-0 PMC 바로가기
The impact of CoronaVac on the neutralization breadth and magnitude of the antibody response to SARS-CoV-2 virusesArticle Published on 2022-09-202022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] 95% confidence interval addition Anti-RBD IgG B.1.1.529 B.1.351 B.1.617.2 Beta booster booster dose booster vaccination Booster vaccine breadth Cohort convalescent COVID-19 patient CoronaVac Critical cross-neutralization Delta dose examined highlight homologous immune response increase in less log magnitude neutralization neutralization breadth neutralization titer neutralize omicron positive proportion receiving recipient reduction reported Result SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus Significant the antibody response vaccination Vaccine variant variants wild-type [DOI] 10.3389/fimmu.2022.990071 PMC 바로가기
A self-assembled trimeric protein vaccine induces protective immunity against Omicron variantArticle Published on 2022-09-172022-11-15 Journal: Nature Communications [Category] SARS, 변종, 진단, [키워드] addition animal models B.1.1.529 Breakthrough infection circulating clinical trials Complete Delta delta variant dose elicited humoral immune response Immune escape immune response in vivo induce L452R Live virus mice mRNA vaccines neutralizing antibody non-human primates omicron predominant prime-boost regimen protective immunity Protein RBD resulting SARS-CoV-2 SARS-CoV-2 S2 SARS-CoV-2 vaccine SARS-CoV-2 variant sequence subunit surpassed sustained T cell T478K Transmissibility trimer trimeric vaccinated individuals vaccination Vaccine vaccine candidate variant variants [DOI] 10.1038/s41467-022-33209-9 PMC 바로가기