flow cytometry – Page 37 – Communicable Diseases Research

Improved Detection of Antibodies against SARS-CoV-2 by Microsphere-Based Antibody Assay

Article

Published on 2020-09-092022-10-29
Journal: International Journal of Molecular Sciences [Category] COVID-19,

Deep immune profiling of COVID-19 patients reveals distinct immunotypes with therapeutic implications

Research Article

Published on 2020-09-042022-10-28
Journal: Science (New York, N.y.) [Category] COVID-19, Fulltext, SARS,

[키워드] activated activating Activation acute COVID-19 acute respiratory syndrome acute viral infection adaptive immune responses affected Analysis Antiviral antiviral response approach available data B cell responses B cell subset B cells Blood caused CD38 CD4 CD4 T cells CD8 CD8 T cell CD8 T cells cells characteristic characterized circulating Clinical data clinical feature Clinical features clinical outcomes collected comparable complex component controls coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 patient COVID-19 severity Cytometry declined deep dimension disease disease severity Donor dysregulation Effective vaccines effector memory exacerbate fatality feature Features flow cytometry fluctuation follicular global pandemic Health healthy donor healthy individual healthy individuals help HLA-DR Hospitalization hospitalized COVID-19 patient hospitalized COVID-19 patients human immune response Human immune system identify immune Immune cell immune modulation immune parameter immune profiling immune response immune system immunological immunology immunotype implicated implication in some individuals Infection infections influence information inhibiting less longitudinal analysis lymphocyte lymphocyte response mechanical ventilation memory B cell mononuclear cell occurred parameters Pathogenesis Patient patients performed peripheral plasmablast plasmablast response progression proliferation proportion remained respond response reveal robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2–specific antibodies Science severe respiratory disease stability STABLE subgroup of patient subset Systems biology T cell T cell activation T cell response therapeutic therapeutic interventions trajectory Treatment UMAP uninfected Vaccine virus with COVID-19
[DOI] 10.1126/science.abc8511 PMC 바로가기 [Article Type] Research Article

Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans

Research Article

Published on 2020-09-042022-10-28
Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS,

[키워드] acute respiratory syndrome affected analyzed Antigen approach Atlanta Bacterial coronavirus Coronavirus disease 2019 correlated COVID-19 COVID-19 patient COVID-19 patients COVID-19 severity disease disease severity expression flow cytometry healthy individual healthy individuals HLA-DR Hong Kong hospitalized COVID-19 patient Human Human immune system human immunity IFN-stimulated genes IFN-α Immune cell immune modulation immune response immune system Immunity immunotype Infection Inflammatory influence information Interferon-α knowledge lack leukocyte mammalian mechanism Mild mononuclear cell myeloid cell parameters Patient PBMC PBMCs performed Peripheral blood plasma plasmacytoid dendritic cells potential therapeutic target Proinflammatory cytokine Rapamycin reduced respond response SARS-CoV-2 Science severe COVID-19 Severe COVID-19 Infection Signaling Single-cell transcriptomics System Systems biology therapeutic interventions TNFSF14 transcriptomics transient expression type I IFNs United States with COVID-19
[DOI] 10.1126/science.abc6261 PMC 바로가기 [Article Type] Research Article

Analysis of type I IFN response and T cell activation in severe COVID-19/HIV-1 coinfection A case report

4850

Published on 2020-09-042022-10-30
Journal: Medicine [Category] COVID-19, SARS,

[키워드] acute respiratory syndrome calculated Case report caused CD38 CD4 CD8 T cell Computed tomography coronavirus Coronavirus-2 COVID-19 described diagnosed enrolled expressing female female patient Fever FIVE flow cytometry Frequency greater Ground-glass opacities healthy individual healthy individuals High-resolution HLA-DR Human immunodeficiency virus human immunodeficiency virus-1 hydroxychloroquine therapy IFN IFN α immune dysregulation immunological individual infected with SARS-CoV-2 interferon leukocyte median mRNA level outcome oxygen Patient patient reported patients PCR assay performed positive Protease inhibitor protease inhibitors regimen respiratory rate reversed SARS-COV-2 infection severe acute respiratory syndrome coronavirus-2 severe pneumonia T cell activation T cell response Treatment Type I IFN uninfected women
[DOI] 10.1097/MD.0000000000021803 PMC 바로가기 [Article Type] 4850

SARS-CoV-2 binds platelet ACE2 to enhance thrombosis in COVID-19

Research

Published on 2020-09-042022-10-29
Journal: Journal of Hematology & Oncology [Category] COVID-19, SARS,

[키워드] ACE2 ACE2 expression aggregates aggregation bind binding blood stream cardiovascular complications CD62P changes in Coagulation factors coagulation parameter condition correlated COVID-19 COVID-19 patient COVID-19 patients Critical Critically ill patient critically ill patients decrease demonstrated develop diagnosed with COVID-19 downstream ENhance evaluated Evidence Ex vivo expressed expression facilitate flow cytometry functional hACE2 healthy volunteers host cell receptor human Ace2 protein hyperactivity immunofluorescence in vitro in vivo inflammatory factors Inflammatory response inhibit SARS-CoV-2 investigated MAPK mice monoclonal antibody MPV Non-COVID-19 PAC-1 pathogenesis of COVID-19 pathway Platelet platelet activation Platelets priming pro-thrombotic Protein provided recombinant responsible Result risk RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus secretion serine protease spike stimulated thrombosis thrombotic event thrombus formation TMPRSS2 transfused transgenic transgenic mice Volume wild-type
[DOI] 10.1186/s13045-020-00954-7 PMC 바로가기 [Article Type] Research

A comparison of four serological assays for detecting anti–SARS-CoV-2 antibodies in human serum samples from different populations

Research Article

Published on 2020-09-022022-10-29
Journal: Science Translational Medicine [Category] COVID-19, MERS, SARS,

[키워드] addition antibody antibody assay antibody profiling Antibody Response Antibody titer Antigen Asymptomatic Cell COVID-19 difference domain ectodomain ELISA assay evaluate expressed flow cytometry full-length healthy blood donors healthy individual Hospitalized hospitalized patient human serum immunoprecipitation individual infected with SARS-CoV-2 LIPS Luciferase immunoprecipitation system Mild mild symptoms neutralization activity Neutralizing activity occurred pandemic performed pilot study Population Prevalence Previous studies profiles Protein pseudotype virus S1 domain SARS-CoV-2 SARS-CoV-2 antigen SARS-COV-2 infection SARS-CoV-2 seroprevalence sensitivity Seroconversion Serological assay serological assays Seropositivity serum sample Severe case severity subpopulation symptom onset symptomatic symptomatic case symptomatic individual the disease the S protein the SARS-CoV-2 trimeric spike Virus neutralization with COVID-19
[DOI] 10.1126/scitranslmed.abc3103 PMC 바로가기 [Article Type] Research Article

A case series of blastic plasmacytoid dendritic cell neoplasia

Article

Published on 2020-09-012024-09-02
Journal: Advances in clinical chemistry [Category] 대상포진,

[키워드] blastic plasmacytoid dendritic cell neoplasm Chemotherapy flow cytometry Transplant
[DOI] 10.37825/2239-9747.1012 PMC 바로가기 [Article Type] Article

SARS-CoV-2 Spike 1 Protein Controls Natural Killer Cell Activation via the HLA-E/NKG2A Pathway

Article

Published on 2020-08-262022-10-31
Journal: Cells [Category] COVID-19, MERS,

[키워드] Activation acute respiratory syndrome affected Alter Analysis Blood co-cultured contrary contribute Control coronavirus degranulation evaluated experiment expressed expression flow cytometry GATA3 HLA-E immune Immunopathogenesis implicated in vitro model Infection inhibitory receptor Interaction lung epithelial cell Lung epithelial cells modulation naïve subject natural natural killer cell NK cell NK cells NKG2A peptide peripheral Protein responsible resulting SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 spike proteins SP1 spike Spike protein transcription factor transfected with viral infections was increased was performed
[DOI] 10.3390/cells9091975 PMC 바로가기 [Article Type] Article

Natural killer cell immunotypes related to COVID-19 disease severity

Research Article

Published on 2020-08-212022-10-28
Journal: Science Immunology [Category] COVID-19, Fulltext, MERS,

[키워드] Activation acute SARS-CoV-2 infection acute viral infection adaptive Analysis Blood Bronchoalveolar lavage Cancer CD56 Cell circulating circulation contribute coronavirus disease COVID-19 COVID-19 disease COVID-19 disease severity COVID-19 patients Defense defined disease pathogenesis disease severity driven by expression Factors flow cytometry hallmark Host host response immunopathology immunotype increase in Inflammatory innate immune innate immune response Last lymphocyte mechanism natural natural killer cell NK cell NK cells Patient patients with moderate Peripheral blood Protein protein-protein interaction provide respond SARS-COV-2 infection severe COVID-19 severe disease severe patients Single-cell RNA sequencing subset understanding viral infection with COVID-19
[DOI] 10.1126/sciimmunol.abd6832 PMC 바로가기 [Article Type] Research Article

A flow virometry process proposed for detection of SARS-CoV-2 and large-scale screening of COVID-19 cases

Special Report

Published on 2020-08-182022-10-31
Journal: Future Virology [Category] COVID-19,