Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification디지털 액적 PCR은 핵산 정제 없이 미정제 용해물에서 SARS-CoV-2 바이러스 로드를 정확하게 정량화합니다.Article Published on 2021-01-122022-09-01 Journal: Scientific Reports [Category] 진단, 치료기술, [키워드] addition approach Biotechnology causative pathogen caused Concordance copy numbers COVID-19 pandemic Diagnosis diagnostic Digital function in viral Infectious disease Infectious-disease diagnostics input Laboratory medium Nasopharyngeal swab nasopharyngeal swab specimen Nasopharyngeal swab specimens nucleic acid Nucleic acid purification offer patients PCR purified RNA qPCR resources RNA extraction robustness SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 viral load SARS-CoV-2 virus sensitivity Surveillance the SARS-CoV-2 virus Transport transport medium utility UTM Variability Viral viral copy number Viral load viral load quantification [DOI] 10.1038/s41598-020-80715-1 PMC 바로가기 [Article Type] Article
Anti-IL6 treatment of serious COVID-19 disease A monocentric retrospective experience심각한 COVID-19 질병의 항IL6 치료 단일 중심적 후향적 경험4900 Published on 2021-01-082022-09-12 Journal: Medicine [Category] 진단, [키워드] acting Acute myocardial infarction acute respiratory distress acute respiratory distress syndrome anti-cytokine anti-IL6 ARDS candidate Candidates clinical trial collected data Complication complications control group coronavirus counterproductive COVID-19 COVID-19 pneumonia cytokine determine died discharged disease drug effective eligible event form framework help Hospitalization Hospitalized identify IL6 Infectious complications Inflammation intensive care interleukin-6 Intervention intravenously Intubated lower mortality medium Mortality multi-organ failure Patient Pneumonia Prognosis Pulmonary thromboembolism reducing respiratory complication Respiratory complications respiratory distress Respiratory failure retrospective risk sarilumab serious COVID-19 severe acute respiratory distress syndrome severe respiratory failure single center Standard of care suffered syndrome systemic level Therapies Thromboembolism Tocilizumab treated Treatment [DOI] 10.1097/MD.0000000000023582 PMC 바로가기 [Article Type] 4900
Dose-dependent response to infection with SARS-CoV-2 in the ferret model and evidence of protective immunity흰 족제비 모델에서 SARS-CoV-2 감염에 대한 용량 의존적 반응 및 보호 면역의 증거Article Published on 2021-01-042022-09-12 Journal: Nature Communications [Category] 변종, 진단, [키워드] animal model bronchopneumonia candidate vaccine clinical disease COVID-19 dosage dose dosed Endpoint endpoints Evidence ferret ferrets groups High dose induce Infection infection with SARS-CoV-2 intranasally low dose lung Lung pathology medium Mild mild infection pathology pfu progression protective immunity Replication respiratory SARS-CoV-2 SARS-COV-2 infection Therapeutics upper respiratory tract URT UTR Viral Viral RNA Viral RNA shedding viral shedding virus Virus shedding [DOI] 10.1038/s41467-020-20439-y PMC 바로가기 [Article Type] Article
Lack of Serologic Evidence of Infection Among Health Care Personnel and Other Contacts of First 2 Confirmed Patients With COVID-19 in Illinois, 20202020년 일리노이주에서 처음 2명의 COVID-19 확인된 환자의 의료 종사자 및 기타 접촉자 간의 감염에 대한 혈청학적 증거 부족Article Published on 2021-01-012022-09-11 Journal: Public health reports (Washington, D.C. : 1974) [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibodies antibody Antibody testing Asymptomatic Atypical Care Characteristics collected collected data Community condition Confirmed Contact Contact tracing coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 determine Dilution dilutions eligible enrolled enzyme-linked immunosorbent enzyme-linked immunosorbent assay Epidemiology Evidence evidence of exposure to Follow-up Health Health care Infection initial Interaction medium Other participant Patient Personal protective equipment PPE provided question questionnaire reported respiratory risk SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody testing SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 spike secondary transmission Seroconversion serologic serologic testing serology serum severe acute respiratory syndrome Coronavirus specimen symptom onset tested Transmission Transmission dynamics virus with COVID-19 [DOI] 10.1177/0033354920966064 PMC 바로가기 [Article Type] Article
Brief Online Cognitive Behavioural Intervention for Dysfunctional Worry Related to the COVID-19 Pandemic: A Randomised Controlled TrialRandomized Controlled Trial Published on 2021-01-012022-10-05 Journal: Psychotherapy and psychosomatics [Category] Coronavirus, SARS, 임상, [키워드] 1:1 Administered assessment baseline behavioural cognitive Cognitive behaviour therapy conducted consequence Controlled COVID-19 COVID-19 pandemic death develop Digital Digital therapy Dysfunctional worry effect size General population improvement Inclusion indicated individual Insomnia intensity intention-to-treat analysis interfere Internet-based treatment. Intervention intervention group Measures medium online psychological intervention participant primary endpoint primary outcome measure Psychological intervention randomised reduce reductions in Registered Satisfaction Serious Adverse Event significantly Swedish Symptom Symptoms Treatment Trial were recorded Worry [DOI] 10.1159/000512843 PMC 바로가기 [Article Type] Randomized Controlled Trial
Knowledge levels among elderly people with Diabetes Mellitus concerning COVID-19: an educational intervention via a teleserviceObservational Study Published on 2021-01-012022-10-05 Journal: Acta Diabetologica [Category] 임상, [키워드] age average body balance Brazil concerning correlation COVID-19 cross-sectional Diabetes Mellitus elderly Elderly patient fixed individual information Intervention involved knowledge Mann-Whitney medium Ministry of Health participant Participants Quantitative rehabilitation response responses social isolation Spearman study participant telehealth the median Type Type 2 diabetes mellitus variable were used women [DOI] 10.1007/s00592-020-01580-y PMC 바로가기 [Article Type] Observational Study
Trick or treat: Social Media’s dissemination power of ophthalmologic information in the pandemic contextReviews Published on 2021-01-012022-10-28 Journal: Romanian Journal of Ophthalmology [Category] Coronavirus, MERS, SARS, [키워드] Care COVID-19 pandemic dissemination Health health care consumer satisfaction information Intervention limitation medium ophthalmology pandemic Patient satisfaction physician physician satisfaction research findings Social Media group synchronous teleconsultation teleophthalmology Trick [DOI] 10.22336/rjo.2021.26 PMC 바로가기 [Article Type] Reviews
Teleconsultation experience with the idiopathic inflammatory myopathies: a prospective observational cohort study during the COVID-19 pandemicObservational Study Published on 2021-01-012022-10-05 Journal: Rheumatology International [Category] SARS, 임상, [키워드] assumed Care change Characteristics clinical Cohort COVID-19 COVID-19 pandemic diagnose effective enrolled feasibility greater identify IMPROVE Inflammatory lockdown management median age medium multicentre Myopathy myositis not different offered outcome over pandemic Patient education prospective observational cohort remote Remote Consultation reported required Rheumatic diseases seeking subset Subtypes teleconsultation the patient Triage WhatsApp [DOI] 10.1007/s00296-020-04737-8 PMC 바로가기 [Article Type] Observational Study
Different Respiratory Samples for COVID-19 Detection by Standard and Direct Quantitative RT-PCR: A Literature ReviewReview Article Published on 2021-01-012022-10-30 Journal: Iranian Journal of Pharmaceutical Research : IJPR [Category] COVID-19, [키워드] acute respiratory syndrome approach collected comparable Contact coronavirus Coronavirus disease 2019 costly cough COVID-19 decrease detection diagnose diagnosis of SARS-CoV-2 diagnostic Diagnostic method Direct HCW HCWs healthcare worker heat inactivation increased risk Infection less literature Lower respiratory tract medium Nasopharyngeal swab NPs oropharyngeal swab Patient patients performed physical proteinase protocol purification Quantitative respiratory respiratory tract samples review RNA extraction RT-qPCR RT-qPCR assay Saliva saliva sample Sample SARS-CoV-2 specimen sputum Standard time-consuming Transmission Transport Treatment upper Viral load Viral RNA [DOI] 10.22037/ijpr.2021.115458.15383 PMC 바로가기 [Article Type] Review Article
Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types하이드로겔 입자는 여러 샘플 유형에서 SARS-CoV-2 RNA의 검출을 향상시킵니다.Article Published on 2020-12-302022-08-31 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] average Biological techniques Biotechnology CDC Cycle threshold value diagnostic EUA hydrogel IMPROVE medium Microbiology nCoV nucleic acid nucleic acid extraction Particle Particles positive sample Qiagen real-time RT-PCR RNA extraction Saliva saliva samples SARS-CoV-2 SARS-CoV-2 RNA tested Transport transport medium Viral Viral load Viral RNA viral titer viral titers virus Volume [DOI] 10.1038/s41598-020-78771-8 PMC 바로가기 [Article Type] Article