The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirusResearch Article Published on 2008-11-282022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] absence analyzed antibody Antigen antigenicity antiviral efficacy assays BALB/c mice cloned coronavirus cross-reactivity determined diagnostic effective drugs effective vaccine ELISA Escherichia coli evaluate expressed expression expression vector fluorescent fusion protein HCoV 229E human coronavirus human coronaviruses humoral IFA immunization immunized mice immunoblot induce inhibit injected injection intranasally Laboratory test management mice National neutralization nucleocapsid OC43 Protein Quantitative reactivity Result RT-PCR SARS patient SARS virus Sensitivity and specificity sera T cell proliferation T-cell analysis T-cell Response vaccine candidate Viral load virus replication was performed [DOI] 10.1186/1471-2180-8-207 PMC 바로가기 [Article Type] Research Article
Plaque assay for human coronavirus NL63 using human colon carcinoma cellsMethodology Published on 2008-11-122022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] African green monkey Antiviral Avicel carboxymethyl-cellulose Cell Cell culture colds Colon coronavirus cytopathogenic effect days post infection demonstrated different disease Drug screening Effects HCoV HCoV-NL63 Human human pathogen humans Infection kidney log NL63 plaque Plaque assay plaque assays produced purification quantification replicated Result Support susceptibility tested utility viral infectivity virus virus infectivity virus replication virus stock [DOI] 10.1186/1743-422X-5-138 PMC 바로가기 [Article Type] Methodology
Functional Genomics Highlights Differential Induction of Antiviral Pathways in the Lungs of SARS-CoV–Infected MacaquesResearch Article Published on 2007-08-102022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] activate activated acute phase acute respiratory distress acute respiratory syndrome Admission Adult against SARS-CoV analyzed Antiviral antiviral signaling Atypical Author cause Cell cells characterized chemokine genes chemokines circumvent Complication coronavirus Critical cynomolgus macaque cytokine demonstrated early IFN signaling Effect event examined functional gene expression profile gene signature Genomics highlight Host host response IFNs IL-8 immune response immunohistochemistry in vitro experiments individuals induction Infection innate immune response Innate immunity intensive care involved IP-10 lung Lung pathology macaque macaques nuclear translocation Pathogenesis pathogenic pathway phosphorylated Pneumonia protective immune response regulated required SARS patient SARS-CoV SARS-CoV infection SARS-CoV–infected cells severe shown Stat1 subset syndrome Transcription transcription factor type I interferon viral infection virus virus replication [DOI] 10.1371/journal.ppat.0030112 PMC 바로가기 [Article Type] Research Article
Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid proteinArticle Published on 2006-08-302022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Antigen assays C-terminus Cell coronavirus cross-reactivity Diagnosis diagnostic early diagnosis epitope expressed FIVE found generate Host IgG immune Infection insect cell linear epitopes mAb modulation monoclonal antibody N protein N proteins N-terminus nucleocapsid nucleocapsid protein pathology phosphorylated preparation Protein reactivity related SARS-CoV SARS-CoV N selected severe Severe acute respiratory syndrome suggested viral pathogenesis virus replication [DOI] 10.1016/j.virusres.2006.07.004 PMC 바로가기 [Article Type] Article