Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosisSARS-CoV-2 진단을 위한 현장 진단 테스트로서의 비색 역전사 루프 매개 등온 증폭의 필수 속성 및 함정Research Article Published on 2021-03-262022-09-10 Journal: Molecular Medicine [Category] SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] addition clinical samples Cluster color change colorimetric detection correlation Ct values demonstrated described detect determinate Diagnosis diagnosis of COVID-19 diagnostic diagnostics Essential false-negative false-negative results indication indications Internal control Interpretation lead Loop-mediated isothermal amplification manuscript molecular Molecular biology molecular diagnostics nuance performed point of care point-of-care positive produced quantification reducing Result resulting reverse transcription RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity supplementary material Symptom technique Viral Viral load virus with color [DOI] 10.1186/s10020-021-00289-0 PMC 바로가기 [Article Type] Research Article
Ultra-fast proteomics with Scanning SWATH Research Published on 2021-03-252022-10-05 Journal: Nature biotechnology [Category] 바이오마커, 치료제, [키워드] accelerate acquisition Biomarker Clinical studies Combination COVID-19 severity experiment experiments facilitate identify increase Isolation large sample Patient plasma plasma proteomics precursor proteome proteomic proteomics quantification Quantitative scanning Tandem mass spectrometry yielding [DOI] 10.1038/s41587-021-00860-4 [Article Type] Research
Light Sheet Microscopy-Assisted 3D Analysis of SARS-CoV-2 Infection in the Respiratory Tract of the Ferret Model흰 족제비 모델의 호흡기에서 SARS-CoV-2 감염의 라이트 시트 현미경 보조 3D 분석Article Published on 2021-03-232022-09-01 Journal: Viruses [Category] SARS, 진단, [키워드] 3D immunofluorescence analyzed cell tropism Clustering confocal laser scanning microscopy COVID-19 distribution ferret ferrets foci focus Host–pathogen interaction host–pathogen interactions host–pathogen interactions immunofluorescence immunohistochemistry in vivo Infection LIGHT light sheet microscopy Localization Microscopy Model morphology optical pandemic paraffin-embedded parameter Pathogens quantification respiratory respiratory pathogen respiratory tract infection SARS-CoV-2 SARS-COV-2 infection Sheet thin sections tissue tissue optical clearing tract upper respiratory tract utility Viral viral pathogen viral pathogens virus Visualization volumetric [DOI] 10.3390/v13030529 PMC 바로가기 [Article Type] Article
Simultaneous quantification of seven repurposed COVID-19 drugs remdesivir (plus metabolite GS-441524), chloroquine, hydroxychloroquine, lopinavir, ritonavir, favipiravir and azithromycin by a two-dimensional isotope dilution LC-MS/MS method in human serum인간 혈청에서 2차원 동위원소 희석 LC-MS/MS 방법을 통한 7가지 용도 변경 COVID-19 약물 렘데시비르(대사산물 GS-441524 포함), 클로로퀸, 히드록시클로로퀸, 로피나비르, 리토나비르, 파비피라비르 및 아지트로마이신의 동시 정량화Article Published on 2021-03-202022-09-11 Journal: Journal of pharmaceutical and biomedical analysis [Category] SARS, 신약개발, 치료제, [키워드] analogue analytical column antiviral therapy Azithromycin Bioanalytical Method Validation Chloroquine Concentration controls COVID-19 COVID-19 pandemic COVID-19 patient develop Dilution drug drug candidate drugs Efficacy and safety European Medicines Agency Favipiravir GS-441524 human serum Hydroxychloroquine inaccuracy injection Isotope Isotope dilution liquid chromatography tandem mass spectrometry (ID-LC–MS/MS) Liquid chromatography Lopinavir LOPINAVIR AND RITONAVIR mass spectrometry metabolite protein precipitation protocol provided quantification quantitative assay Remdesivir Ritonavir robust sensitivity separated serum Seven Simultaneous solid phase Tandem mass spectrometry therapeutic drug Therapeutic Drug Monitoring Treatment two-dimensional Volume were used [DOI] 10.1016/j.jpba.2021.113935 PMC 바로가기 [Article Type] Article
On the SARS-CoV-2 “Variolation Hypothesis”: No Association Between Viral Load of Index Cases and COVID-19 Severity of Secondary CasesMicrobiology Published on 2021-03-162022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] 2019 novel coronavirus acute respiratory syndrome Amedeo Clinical outcome clinical picture collected Contact Coronavirus-2 COVID-19 COVID-19 case COVID-19 emergency cross-sectional study cut-off cycle threshold death Deceased defined diagnosed Diagnosis disease disease severity Evidence exploratory Hospitalization Hospitalized household contact index index case index cases Infection load male median median age Microbiology nasal-pharyngeal swab negative not differ outcomes PCR cycle pediatric Piedmont platform predict presenting Prevalence public health quantification Randomly RdRp gene SARS-CoV-2 SARS-CoV-2 RNA secondary secondary case secondary cases secondary infections Support survived Swab Symptom symptomatic symptomatic and asymptomatic symptomatic infection symptomatic infections tested the SARS-CoV-2 threshold Turin viral amount viral inoculum Viral load [DOI] 10.3389/fmicb.2021.646679 PMC 바로가기 [Article Type] Microbiology
The Quixotic Task of Forecasting Peaks of COVID-19: Rather Focus on Forward and Backward ProjectionsPublic Health Published on 2021-03-162022-10-31 Journal: Frontiers in Public Health [Category] COVID-19, MERS, [키워드] addition Affect change changes in country COVID-19 COVID-19 pandemic Epidemic Epidemiology feature focus Forecasting Forward Germany help highlight Incubation period infected case intrinsic inverse Italy key factor lack Long mathematical mathematical modeling over pandemic peak performed projection quantification reduced reported respond sensitivity analysis shown significant changes in South Korea tested transmission rate transmission rates Uncertainty Uncertainty quantification [DOI] 10.3389/fpubh.2021.623521 PMC 바로가기 [Article Type] Public Health
A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 DetectionSARS-CoV-2 검출을 위해 Cas13a와 통합된 타액 기반 RNA 추출 없는 워크플로Cellular and Infection Microbiology Published on 2021-03-162022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 진단, 치료기술, [키워드] analytical sensitivity applied clinical samples clinical sensitivity comparable COVID-19 COVID-19 testing CRISPR CRISPR Diagnostics CRISPR-Cas13a detection diagnostics healthcare professionals heat inactivation Image analysis include limit of detection nucleic acid pandemic POC point-of-care positive quantification RNA RNA extraction RT-qPCR Saliva saliva samples SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 RNA detection sensitivity step Supply chain test results Treatment turn [DOI] 10.3389/fcimb.2021.632646 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Clinician Attitudes to Using Low-Dose Radiation Therapy to Treat COVID-19 Lung DiseaseCOVID-19 Scientific Communication Published on 2021-03-152022-10-30 Journal: International journal of radiation oncology, biolo [Category] COVID-19, MERS, [키워드] addressed analyzed approach Attitude category Clinical practice clinical setting clinical trial clinician common coronavirus disease COVID-19 current Efficacy ENhance facilitate feasibility identify implementation interview lack Lung disease material Patient patient selection patients with COVID-19 Perspective potential benefit Potential treatment quantification Radiation radiation therapy recruitment required Research resource Result risk subsequent the patient transcribed transfer Transmission Treatment treatment for COVID-19 Trial were recorded [DOI] 10.1016/j.ijrobp.2020.12.003 PMC 바로가기 [Article Type] COVID-19 Scientific Communication
Droplet digital PCR of viral DNA/RNA, current progress, challenges, and future perspectivesReview Published on 2021-03-112022-11-01 Journal: Journal of medical virology [Category] COVID-19, [키워드] addition Concentration digital PCR DNA droplet droplet digital PCR Efficacy feature in viral include inhibitor microfluidic molecular PCR PCR amplification Perspective Poisson distribution polymerase chain proportion quantification reproducibility RNA Sensitivity and specificity standard curve Tolerance virus [DOI] 10.1002/jmv.26846 PMC 바로가기 [Article Type] Review
Neutralization of SARS-CoV-2 with IgG from COVID-19-convalescent plasmaCOVID-19 회복기 혈장에서 IgG로 SARS-CoV-2 중화Article Published on 2021-03-102022-09-01 Journal: Scientific Reports [Category] SARS, 임상, 진단, 치료기술, 치료제, [키워드] administer antibody approach change clinical utility COVID-19 patient COVID-19 patients detectable Dilution elicit endpoints examined fraction greater IgG immune response Immunotherapy inhibited neutralization activity Neutralizing activity plasma plasma sample plasmas producing quantification reduced required SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected patients surrogate the patient viral infection while [DOI] 10.1038/s41598-021-84733-5 PMC 바로가기 [Article Type] Article