Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots오픈 소스 피펫팅 로봇을 활용한 효율적인 SARS-CoV-2 정량적 역전사효소 PCR 타액 진단 전략Article Published on 2022-02-112022-09-11 Journal: Journal of visualized experiments : JoVE [Category] COVID19(2023년), SARS, 치료기술, [키워드] automated Automation average Chain Reaction Clemson University clinical testing Community comparable complex Concentration COVID-19 pandemic diagnostic diagnostic testing efficient epidemiological handling Health Health crisis introduced invasive less Mortality multiplex RT-qPCR nasopharyngeal swabs nucleic acid nucleic acid testing Participants PCR physically polymerase chain polymerase chain reaction Population population surveillance Quantitative reagent Reporting Research reverse transcriptase robot RT-qPCR Saliva SARS-CoV-2 Sensitivity and specificity shown Strategy Supply chain Surveillance thermocycler thermocyclers transcriptase Transmission Viral RNA [DOI] 10.3791/63395 PMC 바로가기 [Article Type] Article
The oxygenation module: the missing link in using sleep apnea devices to treat COVID-19 pneumonia at homeResearch Published on 2022-02-042022-10-28 Journal: BioMedical Engineering OnLine [Category] Coronavirus, COVID19(2023년), Fulltext, MERS, SARS, [키워드] airways Care clinical clinical testing clinically components Concentration COVID-19 COVID-19 patient COVID-19 pneumonia patient COVID-19 positive patients CPAP Critical Critical care early stage equipment healthcare homecare include intensive care units limitation mechanisms NIV Non-invasive non-invasive ventilation oxygen oxygen supplementation oxygen supply Oxygenation PaO patients patients treated Pneumonia positive airway pressure reducing Result sleep apnea Test test result treat Treatment [DOI] 10.1186/s12938-022-00982-z PMC 바로가기 [Article Type] Research
Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection합성 SARS-CoV-2 S 당단백질 바이러스 유사 입자로 면역화하여 원숭이를 감염으로부터 보호Article Published on 2022-01-242022-09-11 Journal: Cell Reports Medicine [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha anti-receptor-binding domain anti-S antibodies Antibody Response Antibody titer Antibody titers approach B cells Beta boost caused CD4 classical clinical testing coated Complete coronavirus COVID-19 cynomolgus macaque cynomolgus macaques formaldehyde formaldehyde cross-linking Gamma Health Health crisis IgA titers IgG Immunity immunization Immunoglobulin Immunoglobulin G induce Infection macaque macaques Nanoparticles nasopharyngeal Neutralizing activity pandemic predominant PROTECT protection Receptor-binding domain receptor-binding domains respiratory S glycoprotein Safe SARS-CoV-2 SARS-CoV-2 S glycoprotein SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus sterilizing immunity T cell T cell responses T helper the vaccine trimer vaccine candidate vaccine strain variant Vesicle Virus-like particle virus-like particles [DOI] 10.1016/j.xcrm.2022.100528 PMC 바로가기 [Article Type] Article
Rapid and accurate clinical testing for COVID-19 by nicking and extension chain reaction system-based amplification (NESBA)NESBA(Nicking and Extension Chain Reaction System-Based Amplification)를 통한 신속하고 정확한 코로나19 임상시험Article Published on 2022-01-152022-09-11 Journal: Biosensors & bioelectronics [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] Accuracy amplification applied Chain Reaction clinical sample clinical samples clinical sensitivity clinical testing COVID-19 detect Diagnosis diagnostics DNA double-stranded Double-stranded DNA extension genomic RNA gRNA identify isothermal isothermal amplification large cohort modulated molecular multiplex N gene NASBA NESBA nucleocapsid platform POC point-of-care primer Primers qRT-PCR Rapid RNA SARS-CoV-2 SARS-CoV-2 viral RNA sequence specificity temperature Viral yielding [DOI] 10.1016/j.bios.2021.113689 PMC 바로가기 [Article Type] Article
Inferring SARS-CoV-2 RNA shedding into wastewater relative to the time of infectionOriginal Paper Published on 2022-01-072022-11-01 Journal: Epidemiology and Infection [Category] COVID-19, SARS, [키워드] acute respiratory syndrome approach average clinical testing coronavirus COVID-19 Disease surveillance environmental monitoring Epidemic IMPROVE individual Infection intensity Interpretation limit pandemic parameter public health surveillance rising RNA SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 transmission Surveillance surveillance data Transmission utility Virus shedding waste water wastewater-based epidemiological monitoring [DOI] 10.1017/S0950268821002752 PMC 바로가기 [Article Type] Original Paper
A Sensitive and Rapid Wastewater Test for SARS-COV-2 and Its Use for the Early Detection of a Cluster of Cases in a Remote CommunitySARS-COV-2에 대한 민감하고 빠른 폐수 테스트 및 원격 커뮤니티에서 사례 클러스터의 조기 탐지에 대한 사용Environmental Microbiology Published on 2022-01-052022-09-10 Journal: Applied and Environmental Microbiology [Category] MERS, SARS, 치료기술, [키워드] absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis Canada cartridge clinical testing Cluster collected Community coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated detection evaluated facilitated faster GeneXpert health authorities IMPROVE increasingly individual Infection input Laboratory less limit of detection molecular MONITOR offer pandemic platform postvaccination powerful tool presence or absence Prevalence provide public health public health authorities public health authority Rapid Rapid test remote respiratory SARS-CoV-2 sensitive severe acute respiratory syndrome Coronavirus Support Surveillance Test tested threshold Transmission triggered unlikely utility wastewater wastewater rapid testing wastewater surveillance Wastewater-based epidemiology Yellowknife [DOI] 10.1128/aem.01740-21 PMC 바로가기 [Article Type] Environmental Microbiology
Drug Combinations as a First Line of Defense against Coronaviruses and Other Emerging VirusesMinireview Published on 2021-12-212022-10-29 Journal: mBio [Category] COVID-19, SARS, [키워드] acute respiratory syndrome antiviral drugs approach approved category A-C pathogens caused clinical disease Clinical efficacy clinical testing clinical trial Combination Contact coronavirus coronavirus disease countermeasures COVID-19 develop drug drug combination drug resistance drug synergy early treatment Ebola virus effective Efficacy effort emerging virus endpoints ENhance highest in vitro indications infected individual lack likelihood limit LINE model-driven approach Novel coronavirus Other pandemic pandemic preparedness Pandemics Prophylaxis regimen SARS-CoV-2 synergistic therapeutic Toxicity treatments for COVID-19 Vaccine viral pandemic viral pathogen viral pathogens virus while [DOI] 10.1128/mbio.03347-21 PMC 바로가기 [Article Type] Minireview
An Intranasal OMV-Based Vaccine Induces High Mucosal and Systemic Protecting Immunity Against a SARS-CoV-2 Infection비강내 OMV 기반 백신은 SARS-CoV-2 감염에 대한 높은 점막 및 전신 보호 면역을 유도합니다Immunology Published on 2021-12-172022-09-11 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, 치료제, [키워드] administer Administered administration Against antibody booster vaccination booster vaccinations candidate vaccine CHO cells clinical development clinical testing control group control groups COVID-19 COVID-19 vaccine COVID-19 vaccines Cramp D614G effective eight greater hamster hamsters Histopathology IgA IgG IgG response immune protection immune response Immunity Immunoglobulin Immunoglobulin G inactivated induce intramuscular intramuscular vaccination intramuscularly intranasal intranasally lesion Local lung Lungs majority membrane vesicles mice mRNA mucosal mucosal immunity Neisseria Neisseria meningitidis Neutralizing antibodies nose offer OMV outer membrane outer membrane vesicle pandemic peptide peptide sequence practicality produced proline Protecting reduced Registered required SARS-CoV-2 SARS-CoV-2 pandemic second vaccination sequence serum Spike protein subunit Subunit vaccine Support systemic the SARS-CoV-2 Transmission vaccination Vaccinations Vaccine variants Vesicle Viral viral entry viral replication Viral vector weight loss [DOI] 10.3389/fimmu.2021.781280 PMC 바로가기 [Article Type] Immunology
Incomplete Subacute Transverse Myelitis Following Vaccination With Pfizer-BioNTech COVID-19 mRNA Vaccine: A Case ReportNeurology Published on 2021-12-162022-10-31 Journal: Cureus [Category] COVID-19, [키워드] adverse effect adverse event Algorithm approval approved association benefit BNT162b2 Brain Case report clinical testing clinical trial coronavirus disease Course COVID-19 COVID-19 vaccine demonstrated detect disorder distribution Efficacy elevated first dose Health healthy humans IgG imperative improvement Incomplete Inflammatory Methylprednisolone MRI mRNA mRNA vaccination mRNA vaccine Myelitis occurred pandemic Patient Pfizer-BioNTech Pfizer-BioNTech COVID-19 vaccine positive potential risk receiving Regulatory report required saddle anesthesia Stage Subacute Surveillance Symptoms temperature the vaccine Tozinameran Transverse transverse myelitis Treatment vaccination vaccination against COVID-19 Vaccinations Vaccine with COVID-19 [DOI] 10.7759/cureus.20460 PMC 바로가기 [Article Type] Neurology
RT-LAMP: A Cheaper, Simpler and Faster Alternative for the Detection of SARS-CoV-2 in WastewaterRT-LAMP: 폐수 내 SARS-CoV-2 검출을 위한 보다 저렴하고 간단하며 빠른 대안Article Published on 2021-12-012022-09-11 Journal: Food and Environmental Virology [Category] SARS, 치료기술, [키워드] amplification analysed clinical testing Concentration concentrations COVID-19 COVID-19 infection COVID-19 infections detection digital PCR epidemiological Epidemiology extracted RNA faster fluorescence detection fluorescent isothermal isothermal amplification LAMP limit of detection Loop-mediated isothermal amplification over plant positive Prevalence protocol reaction RNA RT-LAMP RT-qPCR SARS-CoV-2 Surveillance Transcription Treatment viral infections Viral load viral loads Viral RNA was increased wastewater Wastewater-based epidemiology [DOI] 10.1007/s12560-021-09489-7 PMC 바로가기 [Article Type] Article