Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses다중 심각한 급성 호흡기 증후군 코로나 바이러스의 동시 검출을위한 새로운 1 단계 RT-QPCR 분석의 진단 성능 및 임상 적 타당성Original Article Published on 2022-02-092022-09-10 Journal: Archives of Virology [Category] COVID19(2023년), Fulltext, MERS, SARS, 변종, 치료기술, 치료제, [키워드] Acute respiratory infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis can be used caused cell culture supernatants Chain Reaction circulating variant clinical outcomes clinical sample coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CoVs Critical cross-reaction Diagnosis early diagnosis evaluated event feasibility genes encoding healthy individuals human CoV human CoVs humans IMPROVE in vitro infect Laboratory laboratory diagnosis limit of detection majority membrane mild respiratory symptoms mRNA multiplex RT-qPCR nasal swab Other other coronaviruses Other respiratory viruses patients with COVID-19 polymerase chain polymerase chain reaction Protein qPCR assay Quantitative reaction respiratory respiratory infection Respiratory samples respiratory symptoms respiratory viruses reverse transcription RNA RT-qPCR SARS-CoV-2 SARS-like CoV SARS-like CoVs SARS-related CoV severe acute respiratory syndrome Coronavirus sputum samples supplementary material TCID Transmission zoonotic origin [DOI] 10.1007/s00705-022-05383-0 PMC 바로가기 [Article Type] Original Article
A human antibody reveals a conserved site on beta-coronavirus spike proteins and confers protection against SARS-CoV-2 infection인간 항체는 베타-코로나바이러스 스파이크 단백질의 보존된 부위를 드러내고 SARS-CoV-2 감염에 대한 보호를 제공합니다Research Article Published on 2022-02-082022-09-14 Journal: Science Translational Medicine [Category] MERS, SARS, 진단, 치료기술, [키워드] adopted animal models antibody beta-coronavirus conserved controls coronavirus coronavirus disease CoV COVID-19 COVID-19 infection CoVs cross-neutralizing described description Donor Efficacy epitope exhibit exhibited Fab facilitate helical structure in vivo less lung viral titer mAb molecular basis neutralizing antibody peptide Prophylactic Protective Protective antibody reactivity reagent reduced residue reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S2 Spike protein target therapeutic treat Vaccine design Vaccines weight loss β-CoV β-CoVs [DOI] 10.1126/scitranslmed.abi9215 PMC 바로가기 [Article Type] Research Article
The Landscape of Aminoacyl-tRNA Synthetases Involved in Severe Acute Respiratory Syndrome Coronavirus 2 InfectionPhysiology Published on 2022-01-262022-10-31 Journal: Frontiers in Physiology [Category] COVID-19, [키워드] acute respiratory syndrome addition Amino acid Aminoacyl-tRNA aminoacyl-tRNA synthetase analyzed co-immunoprecipitation co-IP coronavirus coronavirus 2 correlation COVID-19 CoVs cytoplasmic database develop downregulation EARS2 enzyme eukaryotic Evolution expand experiment function HARS2 highly pathogenic human CoVs identify implicated in viral Infection Interaction Interaction network involved key factor landscape M protein mitochondrial mRNA levels Organisms pathway Perspective Phosphorylation physical protein level protein synthesis proteomic provide respiratory SARS-CoV-2 SARS-COV-2 infection Synthetase TARS2 the shortest path transcriptomic translation tRNA [DOI] 10.3389/fphys.2021.818297 PMC 바로가기 [Article Type] Physiology
ERDRP-0519 inhibits feline coronavirus in vitroResearch Published on 2022-01-252022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] Antiviral antiviral drug antiviral efficacy cat caused Cell culture cells complementary coronavirus CoV CoVs CrFK disease dose-dependent manner effective ERDRP-0519 evaluated Feline coronavirus (FCoV) Feline infectious peritonitis Feline infectious peritonitis (FIP) in vitro in vivo inhibit inhibited inhibitor logarithm nucleic acids pathogen polymerase quantification Replication Result significantly supplementary material Treatment Vaccine Viral load Viral RNA virulent virus was reduced [DOI] 10.1186/s12917-022-03153-3 PMC 바로가기 [Article Type] Research
Alphacoronaviruses Are Common in Bats in the Upper Midwestern United StatesArticle Published on 2022-01-192022-10-28 Journal: Viruses [Category] COVID-19, Fulltext, 조류인플루엔자, [키워드] acute diarrhea acute respiratory syndrome alphacoronavirus Analysis analyzed bat bat virus bats characterized circulation common coronavirus CoV CoVs downstream epidemic diarrhea Eptesicus evidenced Genome genome sequence humans Mountain nucleocapsid PCR Protein Proteins Rabies virus RT-PCR SARS-CoV SARS-CoV-2 similarity South South Korea spike gene submitted Surveillance syndrome United State upper virus widespread zoonotic [DOI] 10.3390/v14020184 PMC 바로가기 [Article Type] Article
A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice, rabbits and NHPs새로운 STING 효능제가 첨가된 범-사베코바이러스 백신은 마우스, 토끼 및 NHP에서 강력하고 내구성 있는 중화 항체 및 T 세포 반응을 이끌어냅니다.Article Published on 2022-01-192022-09-11 Journal: Cell Research [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] alum Cellular immune response CoVs Diseases elicit elicited exhibited Final hACE2 highlight highly pathogenic humoral immune responses immunization immunized immunology initial Innate immunity lower airway macaque mice Molecular biology NAb NAbs neutralizing antibody NHP NHPs nine outbreak produced RBD-Fc reduced responses against rhesus macaque rhesus macaques sarbecovirus SARS-CoV SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARSr-CoVs significantly single dose STING T cell T cell response T cell responses upper and lower airways Vaccine Vaccines variant Viral Viral load [DOI] 10.1038/s41422-022-00612-2 PMC 바로가기 [Article Type] Article
Identifying Inhibitors of −1 Programmed Ribosomal Frameshifting in a Broad Spectrum of CoronavirusesArticle Published on 2022-01-182022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] caused coronavirus CoV CoVs disease drug evaluated expression frameshift identifying in viral inhibit inhibited inhibitor Ligand MERS-CoV mRNA negative controls outbreak programmed ribosomal frameshifting provide Recurrent SARS-CoV-2 serine protease inhibitor significantly Spectrum stimulate subset suppressed Therapeutics translation viability Viral proteins virus zoonoses Zoonotic coronavirus [DOI] 10.3390/v14020177 PMC 바로가기 [Article Type] Article
Typing of alpha and beta coronaviruses by DNA barcoding of NSP12 geneResearch Article Published on 2022-01-112022-10-04 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 변종, [키워드] 229E acute respiratory syndrome Alpha Amplicon sequencing amplification amplify applied bat beta coronavirus coding sequence conserved coronavirus Coronaviruses CoV COVID‐19 CoVs DNA DNA bar code Evolution facilitate Host hosts human CoV in silico indicated involved lab Lineage mammal MERS Molecular typing mutant NL63 nucleotide sequence OC43 pandemic pathogenicity Phylogenetic tree resulting SARS‐CoV‐2 sequence Spread taxonomy Transmission typing universal primer variant [DOI] 10.1002/jmv.27550 PMC 바로가기 [Article Type] Research Article
Coronaviruses Nsp5 Antagonizes Porcine Gasdermin D-Mediated Pyroptosis by Cleaving Pore-Forming p30 FragmentResearch Article Published on 2022-01-112022-10-29 Journal: mBio [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] addition Amino acid cleave cleaved coronavirus CoV CoV infection CoVs Critical epidemic diarrhea Evidence facilitate fragment gasdermin D GSDMD hepatic disease humans Infection inhibit initial MERS-CoV molecular mechanism nsp5 pathway PEDV protease pyroptosis Regulation Replication RNA virus SARS-CoV-2 viral replication virus [DOI] 10.1128/mbio.02739-21 PMC 바로가기 [Article Type] Research Article
Photodynamic Inactivation of Human CoronavirusesArticle Published on 2022-01-082022-10-28 Journal: Viruses [Category] COVID-19, [키워드] absence Antiviral antiviral activity Antiviral effect antiviral property assays Betacoronavirus concentrations coronavirus CoVs cultured cell damaged demonstrated digestion dose-dependent manner doses emodin expand experiments HCoV-229E HCoV-OC43 herpes simplex Human human cells human coronavirus inactivation inhibited lentivirus Local microorganisms natural product oxygen PDI photodynamic inactivation photosensitizer PhytoQuin prevented Protein pseudotyped reactive oxygen specy Replication RNase ROS S protein SARS-CoV-2 spike susceptible the SARS-CoV-2 Viral RNA virus viruses [DOI] 10.3390/v14010110 PMC 바로가기 [Article Type] Article