Development of recombinant COVID-19 vaccine based on CHO-produced, prefusion spike trimer and alum/CpG adjuvantsCHO 생산, prefusion 스파이크 삼량체 및 명반/CpG 보조제를 기반으로 한 재조합 코로나19 백신 개발Article Published on 2021-11-262022-09-10 Journal: Vaccine [Category] MERS, SARS, 변종, 비임상, 진단, [키워드] addition adjuvant Aluminium aluminium hydroxide B.1.1.7 B.1.351 B.1.351 variants candidate vaccine CD4 cells challenge China Chinese hamster clinical study COVID-19 pandemic CpG cross-neutralization activity demonstrated development dual effective elicited expressed hamster hydroxide immunogenicity impacted Inflammation inflammation in the lungs lung Lungs mice Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody responses non-human primates Pneumonia prefusion public health purified recombinant COVID-19 vaccine recombinant vaccine reduced robust Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections significantly Spike protein spike trimer Subunit vaccine T cell T cell response These data Trimeric spike protein Vaccine vaccine-induced antibodies vaccine-induced antibody Viral Viral load viral loads [DOI] 10.1016/j.vaccine.2021.10.066 PMC 바로가기 [Article Type] Article
Development of a SARS-CoV-2 Vaccine Candidate Using Plant-Based Manufacturing and a Tobacco Mosaic Virus-Like Nano-ParticleArticle Published on 2021-11-172022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] ACE2 antibody Antibody Response assays association binding block C57BL/6 candidate candidate vaccine coronavirus SARS-CoV-2 COVID-19 COVID-19 pandemic CpG CR3022 Critical development domain effective Epitopes fused Host human IgG1 Fc identity immunogenicity mice monoclonal antibody mosaic nanoparticle neutralizing antibody plant-based manufacturing RBD Receptor-binding domain resulting retained S protein S1 domain SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 vaccine spike glycoprotein STABLE the SARS-CoV-2 These data Tobacco Vaccine vaccine candidate virus virus neutralizing titer virus receptor [DOI] 10.3390/vaccines9111347 PMC 바로가기 [Article Type] Article
An Established Th2-Oriented Response to an Alum-Adjuvanted SARS-CoV-2 Subunit Vaccine Is Not Reversible by Sequential Immunization with Nucleic Acid-Adjuvanted Th1-Oriented Subunit VaccinesArticle Published on 2021-11-012022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] absence acute respiratory syndrome Administered alum adjuvant boost cellular immunity changed coronavirus coronavirus disease COVID-19 CpG demonstrated dose Efficacy eosinophil form Heterologous heterologous boost immunization homogenous boost immunization inactivated induce mice mRNA vaccine nucleic acid adjuvant overcome Pneumonia Prime-boost pulmonary immunopathology question reported response reversed Reversible SARS-CoV-2 SARS-CoV-2 subunit vaccine SARS-CoV-2 vaccine SARS-CoV-2 vaccines subunit Subunit vaccine subunit vaccines Th1 Th1/Th2 orientation Th2 Th2 response Vaccine Vaccines were used [DOI] 10.3390/vaccines9111261 PMC 바로가기 [Article Type] Article
S-farnesylation is essential for antiviral activity of the long ZAP isoform against RNA viruses with diverse replication strategiesResearch Article Published on 2021-10-252022-10-29 Journal: PLoS Pathogens [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Antiviral antiviral activity assist association binding broad inhibitor C-terminal C-terminal region catalytic site catalytically caused cofactor complex coronavirus CpG cysteine cytoplasm Degradation dependent on determinant dinucleotide distribution domain element Endoplasmic reticulum Factors highlight HIV-1 Human immunodeficiency virus hydrophobic in viral inactive Interaction isoform KHNYN lack membrane membranes Microscopy modulate motif N-terminal RNA-binding domain nucleus PARP physical plasma membrane polymerase post-translational modification Prevent Protein provide recruitment regulate replicate Replication required residue restored RNA RNA virus RNAs SARS-CoV-2 Structure subcellular translation TRIM25 type I Vesicle viral pathogens Viral RNA virion virus virus replication ZAP [DOI] 10.1371/journal.ppat.1009726 PMC 바로가기 [Article Type] Research Article
Unmethylated CpG motif-containing genomic DNA fragments of bacillus calmette-guerin improves immune response towards a DNA vaccine for COVID-19메틸화되지 않은 CpG 모티브를 함유한 바실러스 칼멧-게린의 게놈 DNA 단편은 COVID-19에 대한 DNA 백신에 대한 면역 반응을 향상시킵니다Article Published on 2021-10-012022-09-11 Journal: Vaccine [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] 501Y.V2 acute respiratory syndrome acute respiratory syndrome coronavirus addition adjuvant Antibody titers B.1.1.7 Bacillus Calmette-Guérin BALB/c mice BC01 adjuvant caused Cellular immune response coronavirus coronavirus disease coronavirus- COVID-19 CpG disease DNA DNA fragment DNA vaccine effective elicit expressing Genome Genomic DNA humoral immune response immune responses IMPROVE mice neutralize neutralizing antibody Neutralizing antibody titer neutralizing antibody titers pandemic produced respiratory SARS-CoV-2 SARS-CoV-2 spike protein sera Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus Spike protein tested the SARS-CoV-2 unmethylated Vaccine vaccine candidate variants [DOI] 10.1016/j.vaccine.2021.08.103 PMC 바로가기 [Article Type] Article
Preclinical evaluation of a synthetic peptide vaccine against SARS-CoV-2 inducing multiepitopic and cross-reactive humoral neutralizing and cellular CD4 and CD8 responses다에피토프 및 교차 반응성 체액 중화 및 세포 CD4 및 CD8 반응을 유도하는 SARS-CoV-2에 대한 합성 펩타이드 백신의 전임상 평가Research Article Published on 2021-09-272022-09-10 Journal: Emerging Microbes & Infections [Category] SARS, 변종, 진단, [키워드] adjuvant alum Amino acid antibody Antibody Response B-cell epitopes BALB/c CD4 CD8 cellular cellular immunity characterization CpG cross-reactive cysteine cysteines displaying dose epitope epitope mapping Epitopes experiment Freund’s Freund’s adjuvant generate hACE2 HLA humanized humoral identification identify immunization infected individual infected individuals K18-hACE2 mice lethal dose linear epitope linear epitopes mice Neutralizing Neutralizing antibodies neutralizing capacity overlapped peptide peptide vaccine peptides Preclinical Protein Proteins RBD Receptor-binding domain recognizing response S protein S proteins SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants sera subunit suggested T-cell epitopes tested the receptor-binding domain titres transgenic mice vaccination Vaccine vaccine platform [DOI] 10.1080/22221751.2021.1978823 PMC 바로가기 [Article Type] Research Article
Evolutionary Signatures Governing the Codon Usage Bias in Coronaviruses and Their Implications for Viruses Infecting Various Bat SpeciesArticle Published on 2021-09-162022-10-28 Journal: Viruses [Category] COVID-19, [키워드] ancestor appear arginine bat bats beta-CoV beta-CoVs Betacoronavirus Bia cellular Characteristics codon codon usage conserved Coronaviruses CoV CpG CpG dinucleotide demonstrated disease distribution driven by envelope gene Evolution furin cleavage site Genetic highlight Host host tissue Human infecting mammal MERS-CoV MHV-A59 microenvironment molecular murine preference reservoir SARS-CoV SARS-CoV-2 signature Specy survival synonymous Usage variety virus virus genomes [DOI] 10.3390/v13091847 PMC 바로가기 [Article Type] Article
Variability in Codon Usage in Coronaviruses Is Mainly Driven by Mutational Bias and Selective Constraints on CpG Dinucleotide코로나 바이러스에서 코돈 사용의 가변성은 주로 CPG 디 뉴클레오티드의 돌연변이 바이어스 및 선택적 제약에 의해 주도된다.Article Published on 2021-09-102022-09-10 Journal: Viruses [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus adaptation avoidance Bia breadth codon codon usage Codon usage bias comprehensive analysis Coronaviridae family coronavirus coronavirus disease Coronavirus disease 2019 coronavirus genome coronavirus genomes Coronaviruses COVID-19 CpG Critical dependent on dinucleotide Endemic enrichment explain Frequency Host host switch hosts human coronavirus human coronaviruses humans identify infecting mammalian mutational bia Mutational bias Neutral Orthocoronavirinae pandemic primary host respiratory risk SARS-CoV-2 SARS-CoV2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 synonymous the Coronaviridae total variation Usage Variation virus Virus evolution viruses zoonotic [DOI] 10.3390/v13091800 PMC 바로가기 [Article Type] Article
Molecular characterization of SARS-CoV-2 from Bangladesh: implications in genetic diversity, possible origin of the virus, and functional significance of the mutationsResearch Article Published on 2021-08-212022-10-31 Journal: Heliyon [Category] COVID-19, [키워드] accompanied Analysis ancestor B-cell Bangladesh China Chittagong circulating clade Clinical data Complete country COVID-19 COVID-19 pandemic CpG D614G Deletion Diagnosis downloaded Epidemiology epitope European country evade event Evolution functional genetic diversity Genome genome sequence Genome sequencing genomic change GISAID GISAID database Host immune response implication include India insertions isolate isolates less Missense Missense mutation MOST Mutation mutations Nextstrain nucleotide occurred ORF7a ORF8 gene P323L Pathogenesis PCR Phylogeny predict predicted prevalent RdRP reduce reference sequence Region reported reveal RT-PCR SARS-CoV-2 SARS-CoV-2 genome selective sequence severity single nucleotide variations Spike protein submitted synonymous T-cell target region the disease the SARS-CoV-2 virus unique variant viral clearance viral replication virus Wuhan [DOI] 10.1016/j.heliyon.2021.e07866 PMC 바로가기 [Article Type] Research Article
Hidden pandemic: COVID-19-related stress, SLC6A4 methylation, and infants’ temperament at 3 monthsClinical Trial Published on 2021-08-022022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 바이오마커, 임상, [키워드] cells COVID-19 pandemic CpG DNA methylation Effect Epigenetic Human behaviour Infants longitudinal cohort study Methylation mother paediatrics predicted Pregnancy prenatal Serotonin Seven significantly SLC6A4 Stress stress-related gene trauma turn [DOI] 10.1038/s41598-021-95053-z PMC 바로가기 [Article Type] Clinical Trial