Characterization of nanoparticles-based vaccines for COVID-19 Reviews Published on 2022-06-162022-10-05 Journal: Nature nanotechnology [Category] 임상, 치료기술, [키워드] Antigen assays characterization Combination COVID-19 effective vaccine Efficacy Health help immunological intrinsic nanomedicine nucleic acids physicochemical PROTECT Protein Regulatory Safe Streamline Vaccine [DOI] 10.1038/s41565-022-01129-w [Article Type] Reviews
Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes선형 분자 비콘 프로브를 기반으로 하는 RNA 바이러스에 대한 PCR-free 핵산 검사 방법 개발 및 검증Article Published on 2022-06-112022-09-11 Journal: Journal of Nanobiotechnology [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyses applicability bind classical complementary Concentration confocal microscopy coronavirus CSFV Detection limit development diagnostic distribution domain double-stranded FAM Fever fluorophore host cells human diseases linear molecular Molecular beacon probes N genes nucleic acid nucleic acid extraction nucleic acid testing nucleic acids Pandemics PCR-free platform POC point-of-care probe qRT-PCR Quantitative RNA RNA virus RNA viruses SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 N gene severe acute respiratory syndrome Coronavirus supported tissues viral nucleic acid viral nucleic acids virus were used [DOI] 10.1186/s12951-022-01470-1 PMC 바로가기 [Article Type] Article
Disrupting autorepression circuitry generates “open-loop lethality” to yield escape-resistant antiviral agentsArticle Published on 2022-06-092023-07-09 Journal: Cell [Category] COVID19(2023년), [키워드] autoregulatory circuit feedback nucleic acids Synthetic biology transcriptional feedback viral evolution [DOI] 10.1016/j.cell.2022.04.022 PMC 바로가기
Isothermal amplification-assisted diagnostics for COVID-19COVID-19에 대한 등온 증폭 지원 진단Review Published on 2022-06-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] accumulate Accuracy biosensors COVID-19 COVID-19 epidemic diagnostic diagnostics Disadvantages effective equipment example high throughput IMPROVE Infectious disease Infectious diseases integration intrinsic isothermal isothermal amplification isothermal amplification method isothermal amplification methods molecular molecular diagnostics nucleic acid nucleic acid amplification nucleic acid sequences nucleic acids pandemic reached respond SARS-CoV-2 SARS-CoV-2 diagnostics sensitive Sensitive and specific screening. sensitivity Sensitivity and specificity sequence speed temperature the disease [DOI] 10.1016/j.bios.2022.114101 PMC 바로가기 [Article Type] Review
Monitoring COVID-19 spread in Prague local neighborhoods based on the presence of SARS-CoV-2 RNA in wastewater collected throughout the sewer network하수도 네트워크를 통해 수집된 폐수에서 SARS-CoV-2 RNA의 존재를 기반으로 프라하 인근 지역의 COVID-19 확산 모니터링Article Published on 2022-06-012022-09-11 Journal: Water research [Category] COVID19(2023년), SARS, 치료법, [키워드] airport carried Chain Reaction collected correlation COVID-19 COVID-19 case COVID-19 epidemic determined by Epidemic Epidemiology gene encoding hospitals hotspot identify Local monitoring multiplex nucleic acid nucleic acids Occurrence plant polymerase chain polymerase chain reaction Prague Precipitation Quantitative reagent Registered reported reverse transcription RNA isolation RT-mqPCR SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 RNA selected shown Spread Treatment TRIzol™ Reagent upstream was determined wastewater Wastewater-based epidemiology [DOI] 10.1016/j.watres.2022.118343 PMC 바로가기 [Article Type] Article
Enhanced Detection of Viral RNA Species Using FokI-Assisted Digestion of DNA Duplexes and DNA/RNA HybridsDNA 이중체 및 DNA/RNA 하이브리드의 FokI 지원 소화를 사용한 바이러스 RNA 종의 향상된 검출Article Published on 2022-05-102022-09-11 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] addition amplification amplified approaches battery Chain Reaction cleavage Combination complex Critical decrease detection Diagnosis digestion DNA enhanced enzyme fluorescent FokI highlighting human diseases hybrid increase isothermal limit of detection mechanism molecular nucleic acid nucleic acids oligonucleotide oligonucleotides pathogen Pathogens polymerase chain polymerase chain reaction probe proof proof of concept reduced Region reverse transcription RNA RNA molecule RNA molecules Rolling circle amplification SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 variants Sensitivity and specificity Specy ssDNA the SARS-CoV-2 genome viral RNAs [DOI] 10.1021/acs.analchem.2c00407 PMC 바로가기 [Article Type] Article
Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR)Raindance droplet 디지털 PCR(ddPCR)을 사용한 바이러스 핵산의 초고감도 검출 및 정량화Article Published on 2022-05-012022-09-11 Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), SARS, 치료기술, [키워드] animal models Antiretroviral therapy approaches assays caused Cell Combination cure Cure research ddPCR detect Detection sensitivity diagnosis and monitoring digital PCR disease DNA and RNA Efficacy example highlighting HIV HIV prevention HIV-1 HIV-1 infection HIV-1 viral immunodeficiency virus infected individual infected individuals Infection Infectious disease Infectious diseases inhibition inhibitors Interpretation model systems NHP non-human primate nucleic acid Nucleic acid detection nucleic acids outcome platform predictor progression quantification quantitation Raindance reduced required Research reverse transcription rhesus macaque rhesus macaques RNA RT-ddPCR SARS-CoV2 sensitive Simian simian immunodeficiency virus SIV step tissue tissues Treatment treatment intervention treatment interventions viral DNA viral nucleic acid viral nucleic acid detection viral nucleic acids Viral reservoir virus viruses [DOI] 10.1016/j.ymeth.2021.04.025 PMC 바로가기 [Article Type] Article
CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticlesCASCADE: Cas13a와 금 나노입자를 이용한 SARS-CoV-2 육안 탐지Article Published on 2022-05-012022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] acid activate aggregation approach approaches cascade cleave clinical sample clinical samples Concentration COVID-19 pandemic CRISPR CRISPR/Cas13 detect Diagnosis diagnostics Diseases effort gold Gold nanoparticle Gold nanoparticles Infectious diseases isothermal isothermal amplification molecular Naked-eye nanomaterials NASBA nasopharyngeal swabs nucleic acid nucleic acid amplification nucleic acids oligonucleotide Pathogens point-of-care Prevent Protein RNA RNase RPA SARS-CoV-2 SARS-CoV-2 RNA Scientific community sensitivity Spread ssRNA variety Viral viral nucleic acids Viral RNA [DOI] 10.1016/j.aca.2022.339749 PMC 바로가기 [Article Type] Article
Types and Applications of Nicking Enzyme-Combined Isothermal AmplificationNicking 효소 결합 등온 증폭의 유형 및 응용Review Published on 2022-04-212022-09-11 Journal: International Journal of Molecular Sciences [Category] 진단, 치료제, [키워드] amplification amplify application biochemical cleavage clinics Community complexity COVID-19 diagnostic diagnostics Disadvantages Efficiency enzyme Enzymes hospitals Immunodiagnosis IMPROVE Intensive isothermal isothermal amplification limitations molecular molecular detection Molecular diagnosis Nicking nicking enzyme. nucleic acid nucleic acids outbreak overcome platform POCT point-of-care Research shown subject target targets temperature Type [DOI] 10.3390/ijms23094620 PMC 바로가기 [Article Type] Review
Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidateResearch article Published on 2022-04-012022-10-05 Journal: Biochemical Engineering Journal [Category] 임상, 치료제, [키워드] anticipated Antiviral autolysis Biologics manufacturing Broad spectrum antiviral cellular clinical trial Complete contaminant COVID-19 COVID-19 pandemic DNA downstream Downstream recovery E. coli Efficiency endotoxin equipment feature fixed flexible griffithsin host cell hydrolysis IMPROVE membrane Modification nucleic acids pandemic performed produced Protein purified reducing Variation vessel [DOI] 10.1016/j.bej.2022.108403 [Article Type] Research article