Open reading frame – Page 4 – Communicable Diseases Research

Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses

리포터 발현 바이러스를 이용한 생체 내 SARS-CoV-2 감염 역학 분석

423

Published on 2021-10-122022-09-10
Journal: Proceedings of the National Academy of Sciences of [Category] MERS, SARS, 치료법,

SARS-CoV-2 transgressing LncRNAs uncovers the known unknowns

LncRNA를 횡단하는 SARS-CoV-2는 알려진 미지의 것을 밝힙니다.

Editorial

Published on 2021-10-012022-09-11
Journal: Physiological Genomics [Category] SARS, 유전자 메커니즘,

Cloning of a Passage-Free SARS-CoV-2 Genome and Mutagenesis Using Red Recombination

무계대 SARS-CoV-2 게놈의 복제 및 적색 재조합을 사용한 돌연변이 유발

Article

Published on 2021-09-222022-09-10
Journal: International Journal of Molecular Sciences [Category] SARS, 치료기술, 치료법,

[키워드] acute respiratory syndrome Antiviral applied approach BAC Bacterial bacterial artificial chromosome Biology biology of SARS-CoV-2 caused Cell culture chromosome click chemistry clone Cloning Combination concerning contribute coronavirus CoV COVID-19 COVID-19 pandemic disease economic devastation facilitate Features FLAG Frame full-length generate Genetic global effort global efforts incoming viral particles labeling Lambda marker morbidity Mortality nucleocapsid Open reading frame pandemic coronavirus platform powerful tool question recombinant virus Recombination Red red recombination replicated Replication reporter reporter virus respiratory reverse genetics SARS-CoV-2 Severe acute respiratory syndrome Severe Acute Respiratory Syndrome CoV single-cell level spatiotemporal Spread Viral viral particle viral replication Viral RNA viral RNA synthesis virus viruses Visualization
[DOI] 10.3390/ijms221910188 PMC 바로가기 [Article Type] Article

Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals

SARS-CoV-2 게놈 및 COVID-19 개인의 서브게놈 단편의 올리고뉴클레오티드 캡처 시퀀싱

Research Article

Published on 2021-08-252022-09-12
Journal: PLoS ONE [Category] MERS, SARS, 진단,

[키워드] allelic approach capture capture sequencing cause CDC clinical sample clinical samples Complete coronavirus disease Coronavirus disease 2019 COVID-19 Cycle threshold value effective facilitate Frequency full-length genome functional generate Genome genomic hybridization individual infected patients junction methodology mixture offer oligonucleotide oligonucleotide probe Open reading frame ORF1ab gene ORFs Patient qPCR assay Region representing RNA SARS-CoV-2 sequence Sequencing subpopulation suggested the SARS-CoV-2 genome transcriptional activity Transcriptome Viral load viral RNA species viral RNA specy
[DOI] 10.1371/journal.pone.0244468 PMC 바로가기 [Article Type] Research Article

Revelation of Potent Epitopes Present in Unannotated ORF Antigens of SARS-CoV-2 for Epitope-Based Polyvalent Vaccine Design Using Immunoinformatics Approach

면역정보학 접근법을 사용한 에피토프 기반 다가 백신 디자인을 위한 SARS-CoV-2의 주석이 없는 ORF 항원에 존재하는 강력한 에피토프의 계시

Immunology

Published on 2021-08-232022-09-11
Journal: Frontiers in Immunology [Category] MERS, SARS, 변종, 신약개발, 치료법,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus allele Analysis antigen expression antigenic B-cell B-cell epitope B-cell epitopes canonical Canonical protein epitopes carried CD4+ T-cell CD8+ T-cell conformational coronavirus Coverage eight epitope epitope-based polyvalent vaccine Epitopes expressed FIVE followed by highest HLA I supergroups immunodominant immunogenic kill linear linear B-cell epitope linear B-cell epitopes linkers Mutation mutations N-protein Open reading frame open reading frames ORF ORF3c ORF7a Orf9b overlap Polyvalent potent present Protein Region regions respiratory revelation SARS-CoV-2 scenario severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein T-cell T-cell epitope T-cell epitopes the spike protein uORFs Vaccine variant variants
[DOI] 10.3389/fimmu.2021.692937 PMC 바로가기 [Article Type] Immunology

An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Research

Published on 2021-08-182022-10-30
Journal: Journal of Genetic Engineering & Biotechnology [Category] COVID-19,

[키워드] adjuvant Algorithm Amino acid ANN Antigen artificial canine Canine circovirus CAP cause conducted Dog leukocyte antigen epitope epitope vaccine Epitopes Escherichia coli expressed expression gastroenteritis Genome Hemorrhagic immune response immunoinformatics In silico cloning In silico methods in vitro in vivo experiments infections leukocyte linker molecular docking molecular dynamic simulations Molecular dynamics simulation Open reading frame opposite ORFs pathogen peptide-based vaccine performed Protein Result Screening selected selected epitope stability T-cell the vaccine Transcription vaccine construct Vaccine designing Vasculitis were used
[DOI] 10.1186/s43141-021-00220-4 PMC 바로가기 [Article Type] Research

Systematizing the genomic order and relatedness in the open reading frames (ORFs) of the coronaviruses

코로나바이러스의 오픈 리딩 프레임(ORF)에서 게놈 순서와 관련성을 체계화

Article

Published on 2021-08-012022-09-11
Journal: Infection, genetics and evolution : journal of mol [Category] SARS, 유전자 메커니즘, 치료법,

Highly feasible immunoprotective multicistronic SARS-CoV-2 vaccine candidate blending novel eukaryotic expression and Salmonella bactofection

새로운 진핵생물 발현과 살모넬라 박토펙션을 혼합한 매우 실현 가능한 면역보호 멀티시스트론 SARS-CoV-2 백신 후보

Article

Published on 2021-07-222022-09-11
Journal: Journal of advanced research [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단,

[키워드] Analysis anti-SARS-CoV-2 immune responses Antibody titers antigen-specific antibody Antiviral CD4 CD8 Cell-mediated immunity Cellular immune response co-expression COVID-19 Cytometry demonstrated determine develop DNA domain effective Efficacy Efficiency elicited epitope Epitopes eukaryotic expressing expression feasible Flow flow cytometry Frame fused gene delivery heptad repeat highest humoral immune responses immunized mice immunofluorescence assay immunogenicity IMPROVE in vivo increase in investigated mechanism membrane protein mice mRNA Multicistronic expression multivalent vaccine neutralizing antibody titers nsp13 offer Open reading frame pandemic peptide polarization Protein protein expression raised RBD recall Receptor-binding domain recorded RNA robust Safe Salmonella SARS-CoV-2 SARS-CoV-2 gene SARS-CoV-2 genes SARS-CoV-2 neutralizing antibody SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants sera Spike protein splenocyte splenocytes suggested T cell T cells targets Th1 Th1 response Th2 immunity the spike protein the vaccine Vaccine vaccine candidate vaccine construct variant variants were used western blot Western blot analysis
[DOI] 10.1016/j.jare.2021.07.007 PMC 바로가기 [Article Type] Article

Notable sequence homology of the ORF10 protein introspects the architecture of SARS-CoV-2

ORF10 단백질의 주목할만한 서열 상동성은 SARS-CoV-2의 구조를 내부 조사합니다

Article

Published on 2021-06-302022-09-11
Journal: International journal of biological macromolecules [Category] Coronavirus, MERS, SARS, 변종, 진단,

[키워드] acute respiratory syndrome Amino acid amino acids Analysis antigenicity carried caused change clone coil convalescent patient Convalescent patients coronavirus coronavirus disease COVID-19 CTL cytotoxic T lymphocyte decrease epitope Epitopes expressed Fatality rate homology in silico in vivo Intrinsic disorder lymphocyte MERS Missense mutation Missense mutations Mutation mutations nine Open reading frame ORF10 ORF10 protein pandemic pangolin pangolin-CoV Pangolin-CoV-2020 Pathogenesis pathogenesis of COVID-19 pathology physicochemical predicted Protein Proteins reactive respiratory SARS-CoV SARS-CoV-2 sequence sequence homology Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 shift similarity solubility stability strand T cell tyrosine unique variant
[DOI] 10.1016/j.ijbiomac.2021.03.199 PMC 바로가기 [Article Type] Article

Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics

HLA 펩티도믹스를 이용한 제시된 SARS-CoV-2 HLA class I 및 HLA class II 펩티드 식별

Article

Published on 2021-06-292022-09-12
Journal: Cell Reports [Category] MERS, SARS, 진단,