Insights on early mutational events in SARS-CoV-2 virus reveal founder effects across geographical regionsBioinformatics Published on 2020-05-212022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] Affect allele frequency China coronavirus COVID-19 dataset datasets Diagnosis downloaded event founder effect GenBank geographical region Helicase insight Missense Mutation mutations Next-generation sequencing North America ORF1ab gene predominant primer set quantitative PCR Region reverse transcribed RT-qPCR SARS-CoV-2 SARS-CoV-2 sequence SARS-CoV-2 virus sequence SNVs state USA variant Viral genetic drift [DOI] 10.7717/peerj.9255 PMC 바로가기 [Article Type] Bioinformatics
Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2Article Published on 2020-05-182022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] addition can be used collected colorimetric detection contribute Coronaviruses COVID-19 COVID-19 patient cross-reactivity detect Detection limit development Diagnostic method diagnostics disease disease-causing evaluated exhibited H3N2 H5N1 H7N9 HCoV-229E HCoV-NL63 HCoV-OC43 HRV influenza virus isothermal amplification Laboratory less MERS-CoV Molecular diagnosis MPV Novel coronavirus Nucleocapsid gene outbreak primer set Public health response qRT-PCR researcher reverse transcription reverse transcription-loop-mediated isothermal amplification RNA copy RT-LAMP SARS-CoV SARS-CoV-2 Sensitivity and specificity specimen Spread suspected patients the SARS-CoV-2 Transmission Viral RNA virus [DOI] 10.1080/22221751.2020.1756698 PMC 바로가기 [Article Type] Article
Comparison of Commercially Available and Laboratory-Developed Assays for In Vitro Detection of SARS-CoV-2 in Clinical LaboratoriesVirology Published on 2020-04-292022-10-30 Journal: Journal of Clinical Microbiology [Category] COVID-19, SARS, [키워드] acute respiratory syndrome CDC Cepheid Characteristics clinical comparison coronavirus COVID COVID-19 detect detection diagnostic DiaSorin Emergency use FDA fusion gold standard highlight initial Laboratory limit of detection Multiple nasopharyngeal swabs pandemic platform positive primer set public health emergency qPCR reverse transcription-PCR Roche RT-PCR SARS SARS-CoV-2 sensitive assay specimen specimens Viral detection [DOI] 10.1128/JCM.00821-20 PMC 바로가기 [Article Type] Virology
Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay역전사 루프 매개 등온 증폭 분석에 의한 2019 신종 코로나바이러스(SARS-CoV-2)의 신속하고 시각적인 검출Comparative Study Published on 2020-04-082022-09-25 Journal: Clinical microbiology and infection : the official [Category] SARS, 진단, [키워드] 2019 novel coronavirus 95% CI acute respiratory syndrome amplified Clinical diagnosis clinical samples clinically suspected close contact collected coronavirus COVID-19 cross-reactivity detection evaluate evaluated groups isothermal amplification MINI objective observation Patient positive powerful tool primer primer set Rapid Respiratory pathogens Result reverse transcription RNA RT-LAMP RT-PCR S gene SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection sensitivity Sensitivity and specificity specificity specimen suspected patients temperature the mean Total Viral RNA Visual [DOI] 10.1016/j.cmi.2020.04.001 PMC 바로가기 [Article Type] Comparative Study
Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirusArticle Published on 2018-05-122022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] addition ADV, adenovirus amplification amplifying ATCC, American Type Culture Collection BIP, backward inner primer can be used clinical comparable coronavirus CoV, coronavirus cross-reactivity described development diagnostic diagnostic methods DNA Efficacy FFU, focus forming unit FIP, forward inner primer fluorescent Genetic HBoV, human bocavirus HCoV, human coronavirus infections isothermal amplification mechanism MERS MERS case MERS coronavirus MERS-CoV MERS-CoV RNA MERS, Middle East respiratory syndrome Middle East Middle East respiratory syndrome MONITOR MPV, metapneumovirus N, nucleocapsid nucleocapsid ORF, open reading frame ORF1a Patient PBS, phosphate-buffered saline PFU, plaque forming unit PIV, parainfluenza virus primer set probe QProbe or QP, quenching probe Quenching quenching probe Reactions real-time RT-PCR respiratory syndrome coronavirus reverse transcription RSV, respiratory syncytial virus RT-LAMP RT-LAMP, reverse transcription-loop-mediated isothermal amplification Saudi Arabia sequence specimen Strains Surveillance syndrome TCID50, 50% tissue culture infectious dose upE, upstream E [DOI] 10.1016/j.jviromet.2018.05.006 PMC 바로가기 [Article Type] Article
Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)Research Published on 2014-08-082022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] amplification conserved coronavirus described detect detection develop diagnosed Diagnosis Diagnostic method Epidemiologic surveillance exhibited Genetic Genetic diagnostic method genomic infections inoculated isothermal amplification less Meddle East respiratory syndrome (MERS) medium MERS coronavirus (MERS-CoV) MERS-CoV MERS-CoV RNA Middle East nucleocapsid protein occurred outbreak performed Pharyngeal swab Pilot experiment positive primer set real-time RT-PCR required respiratory respiratory syndrome coronavirus Result reverse transcription RT-LAMP Saudi Arabia sensitivity target temperature viral pathogens virus WHO World Health Organization [DOI] 10.1186/1743-422X-11-139 PMC 바로가기 [Article Type] Research
Human coronaviruses are uncommon in patients with gastrointestinal illnessArticle Published on 2010-04-012022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, MERS, [키워드] Adult children clinical clinical manifestation Clostridium difficile collected coronavirus coronavirus detected diarrhea disease emesis Frequency gastrointestinal disease HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HKU1 hospital Human illness implicated included individual infect infectious median neurologic NL63 NO objective occurred Patient pediatric positive positive patient positive sample presenting primer set recorded respiratory complaints respiratory finding respiratory tract Result RNA extraction RT-PCR Sample screened Stool submitted variety Viral gastroenteritis was performed [DOI] 10.1016/j.jcv.2010.03.007 PMC 바로가기 [Article Type] Article
Genetic variability of human coronavirus OC43‐, 229E‐, and NL63‐like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patientsResearch Article Published on 2006-05-232022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] Acute respiratory infection addition Adult age association Bronchiolitis caused cell line children Coinfection coronavirus CoV detect detected diagnosed Diagnosis different distribution found Gene HCoV hospital Hospitalized human coronavirus infections Immunocompromised Immunocompromised patient Immunocompromised patients immunological Inc Infant Infection involved Isolation Lower Lower respiratory tract molecular monoclonal antibodies not found other respiratory virus pathology Patient patients phylogenetics Pneumonia primer primer pair primer set reached Respiratory tract infections RT‐PCR RT‐PCR screened severe respiratory syndrome strain Strains susceptible targeting gene upper respiratory tract Variability were infected [DOI] 10.1002/jmv.20645 PMC 바로가기 [Article Type] Research Article
Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assaysResearch Article Published on 2004-01-092022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] accurate Adenovirus approach assay children compared complications coronavirus spike protein described detect Diagnosis diagnosis of respiratory infection different enterovirus Genes Haemagglutinin Human coronavirus human coronavirus 229E human parainfluenza virus humans Immunocompromised patients immunofluorescence assay Inc include Infection Infectious disease influenza viruses involved Lower respiratory tract multiplex Multiplex assay multiplex RT‐PCR assay OC43 parainfluenza parainfluenza virus PCR assay polyprotein primer set Region respiratory infectious disease respiratory virus reverse transcription rhinovirus rhinoviruses RT‐PCR sensitivity Sensitivity and specificity serotype Simultaneous specificity specimen susceptible viral culture virus viruses [DOI] 10.1002/jmv.20008 PMC 바로가기 [Article Type] Research Article