Protocol for characterizing the inhibition of SARS-CoV-2 infection by a protein of interest in cultured cellsArticle Published on 2022-10-042022-11-15 Journal: STAR protocols [Category] COVID19(2023년), SARS, 진단, 치료제, [키워드] Antiviral candidate Cell Biology Cell-based Assays cells Complete cultured cell cultured cells example Microbiology Molecular biology MUC1 Nucleocapsid gene Protein Protein Bbiochemistry. protocol quantification replication-competent SARS-CoV-2 SARS-CoV-2 SARS-COV-2 infection Transcription viral infection Virus-like particle [DOI] 10.1016/j.xpro.2022.101802 PMC 바로가기
Probing SARS-CoV-2-positive plasma to identify potential factors correlating with mild COVID-19 in Ghana, West AfricaArticle Published on 2022-10-032022-11-15 Journal: BMC Medicine [Category] COVID19(2023년), SARS, 바이오마커, [키워드] ABO ABO blood grouping ABO blood groups acute respiratory syndrome African anti-SARS-CoV-2 antibody antibodies antibody association Asymptomatic Asymptomatic case Blood Blood Group Clustering comparison Concentration coronavirus coronavirus disease correlations COVID-19 COVID-19 severity cytokine cytokine level decrease discrepancy disease severity Eotaxin Factor genetic background group groups Host identify IL-6 IL-8 immune marker Immune profile immune response individuals Kruskal-Wallis Mann-Whitney U test Mild Multiple Multiplex assay offer participant PCR-confirmed performed phenotype plasma Probing proportion quantification recorded reduced regression analysis significantly Spearman statistically significant symptomatic symptomatic and asymptomatic symptomatic patient tested two-tailed was performed was used West West Africa [DOI] 10.1186/s12916-022-02571-2 PMC 바로가기
Estimated Protection of Prior SARS-CoV-2 Infection Against Reinfection With the Omicron Variant Among Messenger RNA-Vaccinated and Nonvaccinated Individuals in Quebec, CanadaArticle Published on 2022-10-032022-11-16 Journal: JAMA Network Open [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] 95% CI adjusted Against age Care case-control study conducted dose doses Estimated female genomic greater Health Heterologous Hospitalization individual individuals Infection laboratory-confirmed laboratory-confirmed SARS-CoV-2 infection messenger Messenger RNA mRNA mRNA vaccination mRNA vaccine Odds ratio Older omicron outcome participant Participants phylogenetically Postinfection Prevent protection provided quantification Quebec, Canada reduction Reinfection risk risk reduction SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant severity Sex significantly higher stratified Surveillance tested vaccinated individual vaccination Vaccine vaccine dose vaccine strain variant [DOI] 10.1001/jamanetworkopen.2022.36670 PMC 바로가기
Diagnostic performance of the SARS-CoV-2 S1RBD IgG ELISA (ImmunoDiagnostics) for the quantitative detection of SARS-CoV-2 antibodies on dried blood spotsArticle Published on 2022-10-012022-11-15 Journal: Journal of Clinical Virology [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] absence analysed Anti-spike antibodies Antibody detection Blood Concentration concentrations correlated correlation cut-off cut-off value dried blood spot Dried blood spots (DBS) ELISA ELISA kit enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (ELISA) Euroimmun evaluate Follow-up humoral IgG IgG ELISA immune responses increasingly International Units/mL MONITOR Pearson correlation performed quantification Quantitative reference method SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 S1 SARS-CoV-2 surveillance sensitivity Sensitivity and specificity serology Serosurveillance serum serum antibody serum samples specificity spot statistical the SARS-CoV-2 vaccination vaccine responses was used [DOI] 10.1016/j.jcv.2022.105270 PMC 바로가기
Comparison of viral propagation and drug response among SARS-CoV-2 VOCs using replicons capable of recapitulating virion assembly and releaseArticle Published on 2022-10-012022-11-15 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome assays Beta changed coronavirus Delta delta VoCs drug resistance drug response ENhance Evolution of SARS-CoV-2 genomes immune evasion inhibitor manifested Mutation novel antiviral drugs Protein provide quantification released remained Remdesivir replicon RNA S gene SARS-CoV-2 sgRNA supernatant Support Transcription variants variants of concern variants of concern (VOC) viral genome replication viral infectivity viral life cycle viral propagation Viral protein Viral-like particle (VLP). viral-like particles virion assembly VLP VoC VOCs WIV04 strain [DOI] 10.1016/j.virs.2022.06.008 PMC 바로가기
The Association of Baseline Plasma SARS-CoV-2 Nucleocapsid Antigen Level and Outcomes in Patients Hospitalized With COVID-19Article Published on 2022-10-012022-11-16 Journal: Annals of internal medicine [Category] COVID19(2023년), SARS, 변종, [키워드] 95% CI acute SARS-CoV-2 infection antibody Antigen baseline Biomarker body mass index Characteristics Clinical outcome clinically COVID-19 cross-sectional study delta variant discharge elevated ENhance enrolled Enrollment evaluate evaluated Factor Government greater higher odd hospital Hospital admission hospital discharge Hospitalized hospitalized patient hospitalized patients identify immunocompromising condition Infectious diseases Inpatient Laboratory less Level Logistic regression Male sex median Multiple multivariable N antigen nasal nasal swab Noninvasive ventilation Odds ratio operation outcome outcomes participant pathogenesis of COVID-19 pathogenesis of SARS-CoV-2 Patient patients with COVID-19 plasma Platform trial point-of-care polymerase chain predict quantification regression models Remdesivir renal impairment SARS-CoV-2 SARS-CoV-2 nucleocapsid severity speed Support Symptoms Therapeutics transcriptase viral replication was determined was measured were assessed worsened worsening [DOI] 10.7326/M22-0924 PMC 바로가기
Digital RT-PCR Chip method for detection of SARS-CoV-2 virusArticle Published on 2022-10-012022-11-15 Journal: Journal of Immunological Methods [Category] COVID19(2023년), SARS, 치료기술, [키워드] applied asymptomatic subject biological sample characterized ChIP Digital Digital RT-PCR limitations Nasopharyngeal swab PCR performed quantification real time recruited RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 viral load SARS-CoV-2 virus sensitivity spike the SARS-CoV-2 virus UNICORN Viral load [DOI] 10.1016/j.jim.2022.113339 PMC 바로가기
Neuropathological findings in fatal COVID-19 and their associated neurological clinical manifestationsArticle Published on 2022-10-012022-11-16 Journal: Pathology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Autopsy Brain calculated case sery CD8 Cell Central nervous system clinical clinical manifestation clinical manifestations correlated correlation COVID-19 described Encephalopathy fatal COVID-19 FIVE highest hospitalisation hypoxic IL-6 illness duration intensive care lesion lesions macrophage activation syndrome microglial microthrombi Neuroinflammation neurological Neurological symptoms Neuropathology no correlation one patient onset of symptoms parameter pathology Patient patients performed Pituitary proportion qRT-PCR quantification retrospective SARS-CoV-2 RNA severe COVID-19 patient systemic inflammation T-cell activation Two patient Vascular [DOI] 10.1016/j.pathol.2022.03.006 PMC 바로가기
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Artificial intelligence-assisted colorimetric lateral flow immunoassay for sensitive and quantitative detection of COVID-19 neutralizing antibodyCOVID-19 중화 항체의 민감하고 정량적인 검출을 위한 인공 지능 지원 비색 측방 유동 면역 분석Article Published on 2022-10-012022-09-12 Journal: Biosensors & bioelectronics [Category] MERS, 진단, 치료기술, [키워드] Algorithm artificial artificial intelligence COVID-19 Detection limit effective Effectiveness ELISA kit evaluate Immunity immunoassays IMPROVE Lateral lateral flow immunoassay LFIA neutralizing antibody platform point-of-care Polydopamine Prevent produced quantification Quantitative serum serum samples spread of COVID-19 the vaccine vaccination Vaccinations Vaccine [DOI] 10.1016/j.bios.2022.114449 PMC 바로가기 [Article Type] Article