Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike proteinMethodology Article Published on 2018-08-202022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antibody applied caused China collected condition cross-reactivity detect development diagnose Diagnosis diarrhea ELISA enzyme-linked immunosorbent epidemic diarrhea IFA Infection pathogen PEDV Porcine epidemic diarrhea virus Protein raising Recombinant spike protein Result Sensitivity and specificity serological serum antibody serum sample Spike protein the disease virus [DOI] 10.1186/s12917-018-1570-5 PMC 바로가기 [Article Type] Methodology Article
Protection against infectious bronchitis virus by spike ectodomain subunit vaccineArticle Published on 2017-09-092023-06-16 Journal: Vaccine [Category] MERS, [키워드] AA, amino acids ANOVA, analysis of variance Ark, Arkansas ArkDPI, Arkansas-Delmarva Poultry Industry coronavirus DOA, day of age DPC, days post-challenge EID50, 50% embryo infectious doses ELISA, enzyme-linked immunosorbent assay HEK293T, human embryonic kidney 293T cells HRP, horseradish peroxidase IBV, infectious bronchitis virus immunization Infectious bronchitis PBS, phosphate buffered saline qRT-PCR, quantitative reverse transcriptase polymerase chain reaction Recombinant spike protein S, spike protein S1, spike S1 subunit S2 SPF, specific-pathogen-free Spike ectodomain [DOI] 10.1016/j.vaccine.2017.09.013 PMC 바로가기 [Article Type] Article
Trigger factor assisted soluble expression of recombinant spike protein of porcine epidemic diarrhea virus in Escherichia coliResearch Article Published on 2016-05-042022-10-27 Journal: BMC Biotechnology [Category] Coronavirus, [키워드] antigens chaperone Chaperone co-expression system cloned co-expression condition contagious develop elicited ELISA epidemic diarrhea Escherichia coli expressed expression expression vectors immune immunization immunogenic Inclusion inclusion bodies mAb mice neutralizing antibody pathogen PEDV produced Protein protein antigen Proteins purified reactivity Recombinant protein recombinant proteins Recombinant spike protein Result S protein sera serum IgG Serum neutralizing antibody spike glycoprotein Strains subunit Subunit vaccine supplementary material tested Trigger Trigger factor Vaccine variety virus western blot [DOI] 10.1186/s12896-016-0268-7 PMC 바로가기 [Article Type] Research Article