Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay outputSARS-CoV-2 감지를 위한 재조합 효소 중합효소 증폭 및 DNA 주형 반응을 이중 형광 및 측면 흐름 분석 출력과 결합Article Published on 2022-04-012022-09-11 Journal: Biopolymers [Category] COVID19(2023년), SARS, 치료기술, [키워드] absence acid acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification applied Biotin chain Chain Reaction coronavirus coumarin diagnostic disease detection DNA DNA templated reaction early disease early phase effective exacerbated Isolation lateral flow lateral flow assay magnitude methodology naked eye nucleic acid nucleic acid sensing. pandemic Patient peptide PNA point-of-care polymerase polymerase chain polymerase chain reaction reaction recombinase polymerase amplification RNA sequence RPA SARS-CoV-2 severe acute respiratory syndrome Coronavirus stimulated the SARS-CoV-2 transfer Transmission Vaccines while [DOI] 10.1002/bip.23485 PMC 바로가기 [Article Type] Article
Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow stripArticle Published on 2021-11-062023-06-14 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), MERS, SARS, [키워드] Influenza virus lateral flow strip recombinase polymerase amplification SARS-CoV-2 Simultaneous identification [DOI] 10.1016/j.bios.2021.113771 PMC 바로가기 [Article Type] Article
An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detectionRNA 검출을 위한 다중 양식과 호환되는 초고속 SARS-CoV-2 바이러스 농축 및 추출 방법Randomized Controlled Trial Published on 2021-10-022022-09-11 Journal: Analytica chimica acta [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Affinity nanoparticles alternative method alternative methods amplification Analysis Chain Reaction characterization characterized clinical samples clinically comparable compatibility Concordance coronavirus Coronavirus-2 COVID-19 pandemic demonstrated diagnoses Dilution dilutions double-blind study Enzymatic extraction form isothermal amplification Laboratory LAMP Loop-mediated amplification (LAMP) Loop-mediated isothermal amplification miniaturization nasopharyngeal swabs Particle pathogenicity polymerase polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive recombinase polymerase amplification Recombinase polymerase amplification (RPA) respiratory reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA eluate RNA isolation RNA virus RPA RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus-2 Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) solution Stage the stage transmission rate Viral viral titer viral titers viral transport media VTM zoonotic [DOI] 10.1016/j.aca.2021.338846 PMC 바로가기 [Article Type] Randomized Controlled Trial
Harnessing recombinase polymerase amplification for rapid multi-gene detection of SARS-CoV-2 in resource-limited settings자원이 제한된 환경에서 SARS-CoV-2의 신속한 다중 유전자 검출을 위한 재조합효소 중합효소 증폭 활용Article Published on 2021-10-012022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification analytical sensitivity complementary complexity conserved COVID-19 pandemic demonstrated detect develop diagnostic diagnostic test diagnostic testing Dipstick E gene equipment faster Genes gold gold standard isothermal amplification Laboratory limit MERS-CoV molecular Molecular assay molecular test Multi-gene nucleic acid testing offer PCR platform point-of-care polymerase RdRP RdRp gene reaction Real-time detection REcombinase recombinase polymerase amplification reverse transcription RNA copies RNA copy SARS-CoV SARS-CoV-2 SARS-CoV-2 genes seasonal coronaviruses Sensitivity and specificity specificity Spread target variants of concern virus [DOI] 10.1016/j.bios.2021.113328 PMC 바로가기 [Article Type] Article
Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification AssayArticle Published on 2021-09-252022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] 3′ untranslated region amplification Analysis canine conserved coronavirus decrease detection Detection limit develop diagnostic DNA amplification effort Endemic evaluate facilitate Fecal sample Feline coronavirus Feline infectious peritonitis FIP gastroenteritis identify Infection isothermal amplification Laboratory nucleic acid Pathogens performed point-of-need testing polymerase Population real-time RT-PCR REcombinase recombinase polymerase amplification resulting reverse transcription RNA RT-PCR RT-RPA running Sensitivity and specificity temperature tested Testing viral burden virus [DOI] 10.3390/pathogens10101237 PMC 바로가기 [Article Type] Article
Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests현장 진료 COVID-19 테스트를 위한 등온 증폭과 결합된 민감한 전기화학 바이오센서Article Published on 2021-06-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, 치료제, [키워드] accumulate acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amplicon amplicons amplification Combination comparable coronavirus COVID-19 COVID-19 test current density diagnosis of COVID-19 Electrochemical detection method equipment gel electrophoresis hybridization isothermal amplification limits Limits of detection N gene PCR platform point-of-care polymerase primer pulse purification RdRP RdRp gene REcombinase recombinase polymerase amplification reduction RPA SARS-CoV-2 sensitive severe acute respiratory syndrome Coronavirus target gene thiol [DOI] 10.1016/j.bios.2021.113168 PMC 바로가기 [Article Type] Article
A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV−2SARS-CoV−2의 신속한 검출을 위한 결찰/재조합효소 중합효소 증폭 분석Cellular and Infection Microbiology Published on 2021-05-282022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 addition addressed amplification caused Chain Reaction China CDC clinical samples comparable Concentration Coronavirus-2 deaths detection Diagnosis DNA equipment less Ligase ligation Mutation nucleic acid Nucleic acid detection optimization ORF1 ORF1ab pandemic pandemic of COVID-19 polymerase polymerase chain polymerase chain reaction Primers principle probe quantitative polymerase chain reaction Rapid reaction recombinase polymerase amplification reported respiratory reverse transcription RNA templates RPA RT-qPCR SARS-CoV-2 sensitivity Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specific biomarker specific biomarkers T4 DNA ligase Transcription Viral Viral RNA virus would increase [DOI] 10.3389/fcimb.2021.680728 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Solid-phase recombinase polymerase amplification using an extremely low concentration of a solution primer for sensitive electrochemical detection of hepatitis B viral DNAArticle Published on 2021-05-012023-06-11 Journal: Biosensors & bioelectronics [Category] B형 간염, [키워드] Electrochemical detection Hepatitis B virus recombinase polymerase amplification Redox cycling Solid-phase amplification. [DOI] 10.1016/j.bios.2021.113065
Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2Vigilant: SARS-CoV2의 측면 흐름 분석 기반 검출을 위한 엔지니어링된 VirD2-Cas9 복합체Letter Published on 2021-04-122022-09-10 Journal: Nano Letters [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] amplification amplified bind biosensors Biotin biotinylated catalytically clinical samples comparable COVID-19 CRISPR CRISPR-Cas9 cross-reactivity dCas9 detect detection disease disease control exhibited FAM Flow fusion inactive lateral flow assay Lateral limit of detection MERS molecular diagnostics nucleic acid Nucleic acid detection offer pathogen Pathogens platform point-of-care polymerase Rapid recombinase polymerase amplification relaxases robust RT-RPA SARS-CoV-2 SARS-CoV2 Sensitivity and specificity sequence this system VirD2 [DOI] 10.1021/acs.nanolett.1c00612 PMC 바로가기 [Article Type] Letter
Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)중증급성호흡기증후군 코로나바이러스 2(SARS-CoV-2)의 신속하고 직접적인 시각적 검출을 위한 역전사 재조합효소 중합효소 증폭 분석법 개발Research Article Published on 2021-01-062022-08-13 Journal: PLoS ONE [Category] 유전자 메커니즘, 치료기술, [키워드] acute respiratory syndrome coronavirus Amplification assay analytical sensitivity carried clinical clinical sensitivity coronavirus coronavirus 2 costly Cross reactivity Detection limit development diagnosing Diagnosis genomic RNA Intervention isothermal amplification lateral flow Nasopharyngeal samples new virus outbreak PCR polymerase polymerase chain polymerase chain reaction positive Rapid real time Real time reverse transcription polymerase chain reaction recombinase polymerase amplification respiratory reverse transcription RNA RPA RT-qPCR RT-qPCR assay RT-qPCR assays RT-RPA SARS-CoV-2 Sensitivity and specificity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specificity SYBR Green SYBR Green I tested viruses [DOI] 10.1371/journal.pone.0245164 PMC 바로가기 [Article Type] Research Article