Single-cell multi-omics sequencing: application trends, COVID-19, data analysis issues and prospects단일 세포 다중 오믹스 시퀀싱: 응용 동향, COVID-19, 데이터 분석 문제 및 전망Article Published on 2021-11-052022-09-11 Journal: Briefings in Bioinformatics [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] Analysis applied approaches bioinformatics Biotechnology Cell complex disease complex diseases complexity component COVID-19 COVID-19 pandemic Data analysis detect Diversity DNA DNA sequencing example genomic information graph graph-based algorithms heterogeneous Increasing individual individual cell individual cells information integrative methods machine learning mechanism Methylation multi pathway Pathways profile Protein question RNA sequence Sequencing Seurat Single Cell single-cell single-cell multi-omics sequencing single-cell sequencing tissue transfer variable variational inference. [DOI] 10.1093/bib/bbab229 PMC 바로가기 [Article Type] Article
Single-Cell RNAseq Profiling of Human γδ T Lymphocytes in Virus-Related Cancers and COVID-19 DiseaseArticle Published on 2021-11-032022-10-28 Journal: Viruses [Category] COVID-19, MERS, [키워드] acute COVID-19 Blood cancer patient Cell children coronavirus disease COVID-19 COVID-19 disease differentiation disease gammadelta healthy Human increase Inflammatory lung lesion lung tissue lymphocyte lymphoma memory mononuclear cell Patient patients Patients with cancer patients with COVID-19 PBMC pediatric Pediatric patient Peripheral blood Profiling promote recruitment RNAseq robust scRNAseq severe COVID-19 disease Single Cell single-cell subset syndrome T cell T cell activation T cell lymphopenia T lymphocyte T lymphocytes therapy tissue trajectory Transcriptome transcriptomic Tumor tumors [DOI] 10.3390/v13112212 PMC 바로가기 [Article Type] Article
Efficient and precise single-cell reference atlas mapping with SymphonyArticle Published on 2021-10-072022-10-04 Journal: Nature Communications [Category] 유전자 메커니즘, [키워드] Algorithm annotation Cell cell type cell types CITE-seq complex Computational models Data integration datasets developmental disease donors downstream efficient expression fetal Hematopoiesis identify increasingly liver memory T cells pancreatic Phenotypes predict recent reference genome Sequencing sequencing read Single Cell single-cell single-cell RNA-seq dataset Software surface protein trajectory transfer [DOI] 10.1038/s41467-021-25957-x PMC 바로가기 [Article Type] Article
High-throughput RNA sequencing of paraformaldehyde-fixed single cellsArticle Published on 2021-09-242022-10-04 Journal: Nature Communications [Category] 유전자 메커니즘, [키워드] Cell cell sorting current detect droplet Express fixation gene expression level Gene expression profiling High-throughput screening Host human betacoronavirus identify inactivation incompatible Infection information methanol OC43 paraformaldehyde pathogen pathogenic pathway permeabilized phenotypic pro-inflammatory Protein RNA RNA sequencing second Single Cell single cells single-cell Single-cell RNA sequencing staining subpopulation subpopulations tissue TMEM119 transcript transcriptomic transcriptomic method Treatment upregulation viral genes viral infection viral reactivation virus [DOI] 10.1038/s41467-021-25871-2 PMC 바로가기 [Article Type] Article
Computational workflow for functional characterization of COVID-19 through secondary data analysis보조 데이터 분석을 통한 CovID-19의 기능적 특성화를위한 계산 워크 플로Protocol Published on 2021-09-242022-09-10 Journal: STAR protocols [Category] SARS, 유전자 메커니즘, [키워드] Abstract Analysis association bioinformatics can not capture Complete complexes COVID-19 Data analysis disease feature functional Gene Expression Genomics Health Sciences heterogeneous identify immunology interactions Meta-analysis molecular mechanism molecular mechanisms multiple model systems network network analysis other diseases outcomes pathophysiological pathophysiology protein interaction protein interactions protocol RNAseq Single Cell single-cell RNA-seq Standard step Systems biology transcriptional transcriptomic underlying disease [DOI] 10.1016/j.xpro.2021.100873 PMC 바로가기 [Article Type] Protocol
Understanding the burden of interstitial lung disease post-COVID-19: the UK Interstitial Lung Disease-Long COVID Study (UKILD-Long COVID)Interstitial Lung Disease Published on 2021-09-232022-10-28 Journal: BMJ Open Respiratory Research [Category] COVID-19, [키워드] alveolar Analysis approvals assessment biochemical breathlessness Bronchoalveolar lavage bronchoscopy clinically Community consequence COVID COVID-19 COVID-19 pandemic deaths determine dissemination dysfunction Epigenetic Ethics exploratory Factor fatigue Gene Expression Genetic Hospital admission hospitalised ILD include indicated Infection interstitial Interstitial Fibrosis Interstitial lung disease lung mitigate MRI observational study Patient patients with COVID-19 peer-reviewed platform Prevalence progression Result RNA Secondary objective Severe infection severity Single Cell Symptoms trajectory transfer understanding Whole blood xenon [DOI] 10.1136/bmjresp-2021-001049 PMC 바로가기 [Article Type] Interstitial Lung Disease
Increased Autotaxin Levels in Severe COVID-19, Correlating with IL-6 Levels, Endothelial Dysfunction Biomarkers, and Impaired Functions of Dendritic Cells중증 코로나19에서 증가된 오토탁신 수치, IL-6 수치, 내피 기능 장애 바이오마커 및 수지상 세포 기능 손상과 상관관계Article Published on 2021-09-162022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 바이오마커, 진단, 치료기술, 치료제, [키워드] ARDS Autotaxin autotaxin (ATX; ENPP2) Biomarkers cohorts correlated COVID-19 COVID-19 pathogenesis COVID-19 patients Cytokine storm dataset dendritic cells dendritic cells (DCs) Dexamethasone endothelial damage ENPP2 expression Extracellular fibrosis function functional homeostasis Hospitalization hyperinflammation IL-6 Immune cell immune cells Impaired include Increased independent Inflammation Level LPA lysophosphatidic acid (LPA) mechanically ventilated mechanically ventilated patient mechanically ventilated patients mRNA level multifunctional Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples pathologic pharmacological pleiotropic pulmonary fibrosis pulmonary injury reduced RNA sequencing scale analysis secreted Serum level severe COVID-19 patients shown Signaling Single Cell suggested suppression Therapeutic benefit therapeutic benefits therapeutic option Treatment vascular dysfunction [DOI] 10.3390/ijms221810006 PMC 바로가기 [Article Type] Article
Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular primingArticle Published on 2021-09-142023-06-03 Journal: Immunity [Category] B형 간염, Fulltext, [키워드] CD8+ T cells Hepatitis B virus imaging interleukin-2 Kupffer cells liver scRNA-seq Single Cell T cell dysfunction Tolerance [DOI] 10.1016/j.immuni.2021.05.005 PMC 바로가기 [Article Type] Article
Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders인간 건강 및 장애에 대한 치료학적 적용을 위한 압타머의 식별 및 엔지니어링Review Published on 2021-09-072022-09-10 Journal: International Journal of Molecular Sciences [Category] 진단, 치료제, [키워드] antibody application approach aptamer aptasensor Biomarker Care chemical properties chemical property Chemistry clearance Cost effectiveness COVID-19 demonstrated diagnostic different disease different diseases digestion disease pathology Engineering Food industry Frame fungal infections Health heterogeneity Human identification Immunotherapy In-vitro initial Lateral flow assay (LFA) ligands maintain Modification molecular Molecular biology molecular imaging Nuclease oligonucleotide physical protein level proteomics renal renal clearance sensitivity Sensitivity and specificity sensor sequence shelf life Single Cell single cell proteomics stem cell stem cells systematic evolution of ligands by exponential enrichment (SELEX) theranostic therapeutic Treatment Variation while [DOI] 10.3390/ijms22189661 PMC 바로가기 [Article Type] Review
SARS-CoV-2 Impairs Dendritic Cells and Regulates DC-SIGN Gene Expression in TissuesSARS-CoV-2는 수지상 세포를 손상시키고 조직에서 DC-SIGN 유전자 발현을 조절합니다Article Published on 2021-08-262022-09-12 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 진단, [키워드] ACE2 airway ANXA1 Blood CD22 cDCs coronavirus SARS-CoV-2 COVID-19 COVID-19 patient COVID-19 patients dataset DC-SIGN DCs dendritic cell dendritic cells downregulation Gene Expression healthy controls host receptor Host receptors immature immune responses indicate innate immune response innate immune responses L-SIGN lung lung DC Lungs MHCII Mild modulation observation pathogenic pDCs plasmacytoid DC plasmacytoid DCs provide SARS Coronavirus SARS coronaviruses SARS-CoV-2 screened Severe case severe cases severe COVID-19 SIGN Single Cell stimulate transcriptomic upper airway [DOI] 10.3390/ijms22179228 PMC 바로가기 [Article Type] Article