Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection programTrizol을 사용한 SARS-COV-2 진단을위한 대체 타액 검사 개발 : 대규모 탐지 프로그램을 찾고있는 소득이 낮은 국가에 적응Research Article Published on 2021-08-182022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] 2019-nCoV added Asymptomatic buffer caused CDC comparable coronavirus costly country development Diagnosis diagnosis of SARS-CoV-2 extraction Families handling healthcare healthcare worker Healthcare workers healthy help infected patients Infection less Mortality Nasopharyngeal swab nasopharyngeal swabs Non-invasive not need Novel coronavirus NPs one patient Panel Patient patient with SARS-CoV-2 patients with SARS-CoV-2 performed Personal protective equipment positive quarantine real-time RT-PCR reduce RNA room temperature RT-PCR RT-qPCR Saliva saliva test SARS-CoV-2 SARS-CoV-2 RNA Schools shown storage temperature Trizol Viral viral inactivation virus [DOI] 10.1371/journal.pone.0255807 PMC 바로가기 [Article Type] Research Article
A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infectionRBD를 표적으로 하는 합성 나노바디는 SARS-CoV-2 감염으로부터 햄스터를 보호합니다Article Published on 2021-07-302022-09-12 Journal: Nature Communications [Category] SARS, 변종, 진단, 치료제, [키워드] ACE2 protein animal model animal models antibody bind binding Bioavailability biochemical candidate Candidates causative agent Clinical sign Clinical signs COVID-19 domain feature form hamster hamsters high affinity high stability host cell in vitro inhibited Interaction Live virus llama mechanism medical intervention nanobodies nanobody neutralization activity Neutralizing Neutralizing antibodies offer outbreak PROTECT pseudoviruses RBD RBD-ACE2 Receptor-binding domain reduce SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudoviruses single dose storage structure-based design sybodies sybody synthetic nanobodies synthetic nanobody therapeutic therapeutic activity Viral RNA viral RNA load virus X-ray crystallography [DOI] 10.1038/s41467-021-24905-z PMC 바로가기 [Article Type] Article
Lyophilization of premixed COVID-19 diagnostic RT-qPCR reactions enables stable long-term storage at elevated temperature사전 혼합된 COVID-19 진단 RT-qPCR 반응의 동결건조는 고온에서 안정적인 장기 보관을 가능하게 합니다Article Published on 2021-07-012022-09-11 Journal: Biotechnology Journal [Category] SARS, 치료기술, [키워드] ambient ambient temperature Cell-free Chain Reaction cornerstone COVID-19 COVID-19 diagnostics COVID-19 outbreak detect diagnostic diagnostic test diagnostic testing diagnostic tests Dilution dilutions elevated temperature eliminating IMPROVE incorporation labor lyophilization maintain Outbreaks point-of-care polymerase chain polymerase chain reaction qPCR quantitative polymerase chain reaction reaction Reactions reduce reducing RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity storage Strategy Synthetic biology temperature tested [DOI] 10.1002/biot.202000572 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain도전적인 사전 분석 조건 하에서 실시간 PCR에 의한 SARS-COV-2의 검출은 면봉 매체와 냉각 체인의 독립성을 나타냅니다.Article Published on 2021-06-302022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] Accuracy Adenovirus Affect Care CDC choice condition detection determined by diagnosis of SARS-CoV-2 Diseases HBSS Health Health care human metapneumovirus immunology independent independent of Influenza influenza A Laboratory media Medical research medium métapneumovirus Microbiology Molecular biology other respiratory virus Other respiratory viruses PCR question Real-time PCR reliability Resilience respiratory respiratory specimens respiratory syncytial virus respiratory viruses reveal rhinovirus rising RT-PCR Saline SARS-CoV-2 SARS-CoV-2 testing specimen storage storage conditions Swab temperature Transport Transport media transport medium Universal UTM Viral Viral detection viral transport media viruses VTM [DOI] 10.1038/s41598-021-93028-8 PMC 바로가기 [Article Type] Article
Efficient and effective single-step screening of individual samples for SARS-CoV-2 RNA using multi-dimensional pooling and Bayesian inference다차원 풀링 및 베이지안 추론을 사용하여 SARS-CoV-2 RNA에 대한 개별 샘플의 효율적이고 효과적인 단일 단계 스크리닝Life Sciences–Chemistry interface Published on 2021-06-162022-09-10 Journal: Journal of the Royal Society Interface [Category] MERS, SARS, 진단, 치료기술, [키워드] Algorithm Bayesian Bayesian inference Chain Reaction COVID-19 pandemic Critical detect effective efficient faster Health implementation Infectious disease Metrics molecular molecular assays Monte Carlo non-adaptive screening outcome Pathogens polymerase chain polymerase chain reaction pooling positive result protocol public health emergency Rapid required resource reverse transcription RT-PCR SARS-CoV-2 SARS-CoV-2 RNA scaled up screened screening strategy Sensitivity and specificity specimen storage Support Surveillance sustained tested Threats undergo Viral viral pathogen viral pathogens Volume widespread [DOI] 10.1098/rsif.2021.0155 PMC 바로가기 [Article Type] Life Sciences–Chemistry interface
Validation of a multiplex flow immunoassay for detection of IgG antibodies against SARS-CoV-2 in dried blood spots건조된 혈반에서 SARS-CoV-2에 대한 IgG 항체 검출을 위한 다중 흐름 면역분석의 검증Research Article Published on 2021-05-282022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, 치료법, [키워드] analyzed anti-SARS-CoV-2 anti-SARS-CoV-2 IgG Anti-viral antibodies antibody Antibody detection attribute authorization Blood clinical testing Community comparable complementary Concordance detect dried blood spot drug Elecsy Elecsys ELISA Emergency Emergency use authorization EUA expansion FDA food Food and Drug Administration IgG antibody immunoassay Interpretation magnetic bead Magnetic beads multiplex nucleocapsid overall concordance Pattern recognition plasma provide provided Receptor binding domain reference method Result Roche Diagnostics S1 subunit SARS-CoV-2 SARS-CoV-2 antibody sera serologic testing serum serum and plasma specimen storage tested thresholds validation Viral Viral antigen viral antigens [DOI] 10.1371/journal.pone.0252621 PMC 바로가기 [Article Type] Research Article
The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland핀란드 헬싱키의 폐수 유입수에서 SARS-CoV-2 RNA 바이오마커의 검출 및 안정성Article Published on 2021-05-202022-09-11 Journal: The Science of the total environment [Category] MERS, SARS, 바이오마커, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Analysis analyzed Betacoronavirus Biomarker collected conditions copy numbers coronavirus COVID-19 disease decay Decay characteristics fraction Frequency Helsinki IMPROVE influent linear lockdown lockdowns many cases MONITOR Municipal sewage New New coronavirus Norovirus Particulate matter persistence Prevalence quantified quantitative PCR remained respiratory RNA RNA copy number RNA copy numbers RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus stability statistically significant storage storage condition storage conditions supernatant Surveillance temperature the SARS-CoV-2 the SARS-CoV-2 virus Treatment Ultrafiltration was tested wastewater water [DOI] 10.1016/j.scitotenv.2021.145274 PMC 바로가기 [Article Type] Article
The Biobanque québécoise de la COVID-19 (BQC19)—A cohort to prospectively study the clinical and biological determinants of COVID-19 clinical trajectoriesBiobanque québécoise de la COVID-19(BQC19) - COVID-19 임상 궤적의 임상 및 생물학적 결정 요인을 전향적으로 연구하기 위한 코호트Research Article Published on 2021-05-192022-09-12 Journal: PLoS ONE [Category] MERS, SARS, 진단, [키워드] addition analyses anti-SARS-CoV-2 antibody approved Canada circulating Clinical data clinical variables Cohort Community coordination coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 crisis death determinant disease DNA and RNA effort global pandemic health authorities Health Organization Hospitalized immediate action include individuals Infection International limit Local magnitude management Manifestations marker metabolome mononuclear cell multiplex National Novel coronavirus novel coronavirus disease organized outbreak pandemic patients Peripheral blood Peripheral blood mononuclear cells plasma positive proteome Research researcher responses responsible SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 PCR serum Spectrum storage trajectory Transcriptome transcriptome sequencing uninfected urgency Whole blood whole genome World Health Organization [DOI] 10.1371/journal.pone.0245031 PMC 바로가기 [Article Type] Research Article
Impaired performance of SARS-CoV-2 antigen-detecting rapid diagnostic tests at elevated and low temperatures고온 및 저온에서 SARS-CoV-2 항원 검출 신속 진단 검사의 성능 저하Article Published on 2021-05-012022-09-10 Journal: Journal of Clinical Virology [Category] 진단, 치료기술, [키워드] Ag-RDT Ag-RDTs ambient ambient temperatures analytical sensitivity Antigen Antigen-detecting rapid diagnostic tests Cell culture cell culture supernatant clinically complement condition conditions consequence COVID-19 culture supernatant diagnostic tests diagnostics eight elevated elevated temperature Emergency use exhibited exposure to false-negative false-positive result False-positive results FIVE Genome Health Organization Highlights impair Impaired include Incubation limit limits Limits of detection low temperature Low temperatures mean temperature mean temperatures molecular molecular diagnostics North not affected occurred operation pandemic quarantine ranged rapid antigen test Rapid diagnostic test RDTs reduce reduced respiratory respiratory virus respiratory viruses SARS-CoV-2 SARS-CoV-2 cell sensitivity South specificity storage temperature Temperature stability test results tested the WHO Transmission Tropics virus concentration Virus concentrations viruses Winter World Health Organization [DOI] 10.1016/j.jcv.2021.104796 PMC 바로가기 [Article Type] Article
Self-sampling of capillary blood for SARS-CoV-2 serologySARS-CoV-2 혈청학을 위한 모세혈관 자가 샘플링Article Published on 2021-04-082022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] 95% CI analysed Analysis antibody Blood blood collection capillary capillary blood capillary blood samples Cohen’s kappa coefficient collected Concordance COVID-19 detect Diagnosis Dried blood spots eight Epidemiology feasible Filter Paper heparin IgG ELISA immune response immunology Infectious diseases Laboratory Large-scale testing Lateral flow tests lithium Lithium heparin nine not affect participant plasma powerful tool reference standard room temperature SARS-CoV-2 sensitivity serological assessment Serological testing serology storage temperature Transmission venepuncture venous venous blood viral infection [DOI] 10.1038/s41598-021-86008-5 PMC 바로가기 [Article Type] Article