Improved sensitivity, safety, and rapidity of COVID-19 tests by replacing viral storage solution with lysis bufferResearch Article Published on 2021-03-302022-10-28 Journal: PLoS ONE [Category] COVID-19, SARS, [키워드] clinical sample collected Conducting COVID-19 pandemic COVID-19 patient COVID-19 test COVID-19 tests did not reduce Efficiency equipment healthy controls IMPROVE Improved lysis buffer MONITOR nasopharyngeal swabs not affect nucleic acid Patient PCR cycle PCR test replacing RNA RNA extraction RT-qPCR test SARS-CoV-2 SARS-CoV-2 detection sensitivity significantly more Swab temperature tested threshold virus VTM [DOI] 10.1371/journal.pone.0249149 PMC 바로가기 [Article Type] Research Article
Criticality of In-House Preparation of Viral Transport Medium in Times of Shortage During COVID-19 PandemicCOVID-19 팬데믹 기간 동안 바이러스 수송 매체의 자체 준비의 중요성Article Published on 2021-03-152022-09-12 Journal: Laboratory medicine [Category] SARS, 진단, [키워드] Balanced bovine Clinical pathology Community conducted COVID-19 COVID-19 pandemic diagnostic Efficacy emergency preparedness FBS feasible fetal HBSS heat-inactivated Laboratory laboratory workflow manuscript molecular Molecular pathology preparation provide Quality assurance Quality control salt SARS-CoV-2 serum solution specimen stability sterility supply shortage time viral transport media viral transport media preparation. Viral transport medium VTM were assessed [DOI] 10.1093/labmed/lmaa099 PMC 바로가기 [Article Type] Article
Optimal preparation of SARS-CoV-2 viral transport medium for culture배양을 위한 SARS-CoV-2 바이러스 수송 배지의 최적 준비Short Report Published on 2021-03-102022-09-01 Journal: Virology Journal [Category] SARS, 변종, 진단, 치료기술, [키워드] Analysis antibody approaches best competent COVID-19 pandemic Culture Diagnosis diagnostic diagnostic protocol diagnostic testing drug Effect escape mutants filtered handling inoculated investigated Isolation Laboratory medium mutants nucleic acid Nucleic acid detection preparation provided quantitative PCR Replication required Result robust room temperature SARS-CoV-2 SARS-CoV-2 viral Sequence analysis Sterilization supply chains Support supported susceptibility temperature tested the preparation Transport Transport media transport medium Vaccine Vero cells Viral viral culture viral replication Viral transport medium virus VTM while [DOI] 10.1186/s12985-021-01525-z PMC 바로가기 [Article Type] Short Report
Copan eNAT Transport System To Address Challenges in COVID-19 Diagnostics in Regions with Limited Testing AccessCopan eNAT Transport System, 테스트 접근이 제한된 지역의 COVID-19 진단 문제 해결Virology Published on 2021-02-122022-09-12 Journal: Journal of Clinical Microbiology [Category] MERS, 진단, [키워드] absence access Accuracy address biosafety Brescia can not Care Cell culture Cepheid Cepheid Xpert Xpress SARS-CoV-2 challenge clinics cold chain compatibility condition coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 testing cycle threshold demonstrated diagnostic Diagnostic accuracy diagnostics eNAT evaluated expand handling Health Health care hospital implementation Italy Limited limited testing access low-resource settings lysate medium molecular nasopharyngeal PBS phosphate phosphate-buffered saline point of care reduced Region regions respiratory respiratory virus rural areas rural healthcare Saline SARS-CoV-2 SARS-CoV-2 inactivation SARS-CoV-2 specimen stability sensitivity specimen specimen stability stability Support syndrome System temperature Testing Transport Transport media transport medium Viral viral inactivation Viral transport medium VTM [DOI] 10.1128/JCM.00110-21 PMC 바로가기 [Article Type] Virology
Feasibility of using alternative swabs and storage solutions for paired SARS-CoV-2 detection and microbiome analysis in the hospital environmentResearch Published on 2021-01-222022-10-29 Journal: Microbiome [Category] COVID-19, [키워드] 16S rRNA gene Abstract analyses Analysis Antibiotics collected COVID COVID-19 COVID-19 testing demonstrated detect Determining diagnostic driven by evaluated experiment FIVE global health Government hospital ICU impacted in viral include indicated infections initiative intensive care Laboratory lack limit of detection microbial community Microbiome nasal Particles Patient patients performed public health Result RNA RNase RNases RT-qPCR SARS-CoV-2 SARS-CoV-2 detection Sequencing supplementary material Swab swabs tested the patient transmission of SARS-CoV-2 Transport media transported Viral particles virus VTM was measured [DOI] 10.1186/s40168-020-00960-4 PMC 바로가기 [Article Type] Research
A comparison of health care worker-collected foam and polyester nasal swabs in convalescent COVID-19 patientsResearch Article Published on 2020-10-272022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] average Care collected Combination Concordance convalescent COVID-19 patient correlated cycle threshold Health in viral media nasal swab polyester sensitivity Swab threshold Transport media Viral load VTM [DOI] 10.1371/journal.pone.0241100 PMC 바로가기 [Article Type] Research Article
Evaluation of Commercially Available Viral Transport Medium (VTM) for SARS-CoV-2 Inactivation and Use in Point-of-Care (POC) TestingArticle Published on 2020-10-232022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] addition applied buffer conducted described determine diagnostic experiment inactivated inactivation incubated molecular non-infectious physically platform POC point-of-care protocol SARS-CoV-2 SARS-CoV-2 virus separated specimen Testing titre Transport viability viral inactivation virucidal virus VTM WHO World Health Organization [DOI] 10.3390/v12111208 PMC 바로가기 [Article Type] Article
A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource SettingsArticle Published on 2020-09-242022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acute SARS-CoV-2 infection affecting caused clinical sensitivity collected country COVID-19 COVID-19 testing COVID-19 tests demonstrated equipment Evidence extracted RNA extraction step globe gold standard heat-inactivated help Laboratory Low resource setting Molecular assay Molecular diagnosis outbreak pandemic Patient PCR performed polymerase chain positive producing Rapid rapid RT-PCR assay Raspberry Pi Real-time PCR reduced resource reverse transcription RNA RNA extraction RNA isolation RT-PCR RT-PCR assay RT-qPCR SARS-CoV-2 RT-PCR SARS-CoV-2 virus setting significantly specimens Spread the mean thermal cycler virus transport medium VTM [DOI] 10.3390/diagnostics10100739 PMC 바로가기 [Article Type] Article
Rapid isothermal amplification and portable detection system for SARS-CoV-2530 Published on 2020-09-152022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Alternative pathway amplify cartridge characterized clinical sample clinical samples Complete controls coronavirus COVID-19 diagnostics COVID-19 pandemic detect detection system Diagnosis diagnostic test diagnostics example gold standard inhibiting Isolation isothermal isothermal amplification labor-intensive Laboratory limit of detection nasal fluid negative sample performed platform POC POC test point-of-care positive sample primer protocol provide Rapid required reverse transcription RNA copy RNA extraction RNA sequences RT-LAMP RT-PCR SARS-CoV-2 SARS-CoV-2 virus sequence Significance smartphone reader Swab time-consuming Viral transport medium virus Volume VTM was tested while [DOI] 10.1073/pnas.2014739117 PMC 바로가기 [Article Type] 530
A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229EShort Report Published on 2020-08-252022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] 229E amplification assays bypassing can be used coronavirus detect virus HCoV-229E homogenization human coronavirus human coronavirus 229E in vitro limit of detection methodology nucleocapsid PCR PCR assay polymerase chain processed provide purification reductions in required resource Result resulting RT-qPCR sensitivity serial dilution specimen Swab swabs transfer Viral detection viral diagnostics viral infection viral transport media virus detection VTM [DOI] 10.1186/s12985-020-01405-y PMC 바로가기 [Article Type] Short Report