Development and validation of an LC-MS/MS method for determination of hydroxychloroquine, its two metabolites, and azithromycin in EDTA-treated human plasmaResearch Article Published on 2021-03-052022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] active against acute respiratory syndrome antimalarial drug blood sample clinical study conditions coronavirus COVID-19 pandemic develop development Dilution emergency response ESI freeze-thaw cycles HCQ Human plasma Hydroxychloroquine injected Ion LC-MS/MS system less Liquid chromatography m/z matrix effect metabolite metabolites normalized overcome pandemic quantification quantitation ranged reported Result Sample SARS-CoV-2 selected Tandem mass spectrometry temperature Total Volume was used were used [DOI] 10.1371/journal.pone.0247356 PMC 바로가기 [Article Type] Research Article
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodiesArticle Published on 2021-03-042022-10-04 Journal: Scientific Reports [Category] SARS, 바이오마커, [키워드] Accuracy acute respiratory syndrome Admission Analytical biochemistry anti-SARS-CoV-2 IgG antibody chemiluminescence clinical collected convalescent Coronavirus-2 detect detection diagnostic Effectiveness enzyme evaluated help IgG IgG and IgM IgM IgM antibody immunoassay Immunological techniques Infectious-disease diagnostics MERS-S negative sample not correlated Nucleocapsid proteins platform profile Proteins quantification Quantitative quantitative range reproducibility SARS-CoV-2 SARS-CoV-2 IgG SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 vaccine SARS-CoV-2-infected patient SARS-CoV-2-infected patients SARS1 serological test serum sample severity Spike protein [DOI] 10.1038/s41598-021-84387-3 PMC 바로가기 [Article Type] Article
Quantitative and semi-quantitative CT assessments of lung lesion burden in COVID-19 pneumoniaCOVID-19 폐렴에서 폐 병변 부담의 양적 및 반정량적 CT 평가Multicenter Study Published on 2021-03-042022-09-01 Journal: Scientific Reports [Category] 진단, 치료법, [키워드] All the patient all the patients applied assessment assigned clinical evidence clinically Computed tomography COVID-19 COVID-19 patient COVID-19 patients COVID-19 pneumonia Diagnostic markers hospitals individual level Inflammatory inflammatory lesions lesions lobar Local lung lung lesion lung lesions Medical imaging Mild opacity Patient patients Pneumonia quantification Quantitative quantitative assessment Radiography recruited Respiratory tract diseases semi-quantitative sensitivity significantly higher suggested true negatives true positive Volume [DOI] 10.1038/s41598-021-84561-7 PMC 바로가기 [Article Type] Multicenter Study
Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal SwabsResearch Article Published on 2021-03-022022-10-31 Journal: Disease Markers [Category] COVID-19, SARS, [키워드] Accuracy acute respiratory syndrome Concentration containment measures coronavirus Department Digital digital PCR E-gene Efficacy evaluate identify Infection Italy limitation Medicine methodology Molecular biology nasopharyngeal swabs nucleic acid overcome pandemic Patient PCR Precision problems quantification quantitative reverse transcription-PCR reproducibility Result robust RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity specificity Spread subjects Symptom the SARS-CoV-2 university validation Viral RNA [DOI] 10.1155/2021/8890221 PMC 바로가기 [Article Type] Research Article
Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNAArticle Published on 2021-03-022022-10-04 Journal: Journal of virological methods [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] application arising clinically coding region coding regions complex coronavirus coronavirus transcription Course diagnostic discontinuous transcription Droplet-digital PCR ENhance full length Gene Expression Genome genomic genomic RNA gRNA hallmark Infection novel positive-sense quantification Quantitative Quantitative assays Region RNA SAR-CoV-2 SARS-CoV-2 SARS-CoV-2 RNA semi-quantitative sequence sgRNAs subgenomic mRNA subgenomic RNAs target region Transcription Viral transcription/replication [DOI] 10.1016/j.jviromet.2021.114115 PMC 바로가기 [Article Type] Article
Plastic residues produced with confirmatory testing for COVID-19: Classification, quantification, fate, and impacts on human healthShort communication Published on 2021-03-012022-10-05 Journal: The Science of the total environment [Category] SARS, 진단, [키워드] Air pollution analyzed classification confirmed cases Control coronavirus country COVID-19 COVID-19 pandemic COVID-19 test cross-contamination Diagnosis diagnostic test emission Environment impacts evidenced generate Health human health humans Impact IMPROVE increasingly international organization Patient plant plastic plastic waste Prevent produced quantification Real-time PCR recommendation reduce released residue residues RT-PCR SARS-CoV-2 Scientific community Spread tested [DOI] 10.1016/j.scitotenv.2020.144167 [Article Type] Short communication
Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronavirusesArticle Published on 2021-02-252022-11-01 Journal: Journal of virological methods [Category] COVID-19, MERS, SARS, [키워드] Accuracy Analysis antibody Antigen automated binding capture antigen clinical clinical trials coronavirus CoV COVID-19 COVID-19 vaccine development demonstrated Endemic fluorescent human coronavirus human CoV human serum IgG IgG antibody immunoassay limit linear Microarray pandemic positive Precision produced quantification Rapid replicate representing RT-PCR SARS-CoV-2 serological analysis Serological assay serology serum Spike protein Streamline VaxArray [DOI] 10.1016/j.jviromet.2021.114111 PMC 바로가기 [Article Type] Article
Neutrophil Extracellular Traps in SARS-CoV2 Related Pneumonia in ICU Patients: The NETCOV2 StudyMedicine Published on 2021-02-232022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] acute respiratory syndrome Analysis association Blood Blood level Coagulation Concentration coronavirus COVID-19 patients COVID19 decrease defined described died disease Extracellular extracellular trap flow cytometry Hypoxemia ICU ICU admission Infectious disease Inflammatory inflammatory status intensive care lack male median Mortality multivariable NET NETs neutrophils extracellular traps neutrophils extracellular traps PaO pathway Patient patients who survived Pneumonia Primary outcome quantification quantified remained Respiratory failure SARS-CoV-2 SARS-CoV2 severe hypoxemia significantly therapeutic target Whole blood whole blood sample [DOI] 10.3389/fmed.2021.615984 PMC 바로가기 [Article Type] Medicine
Seroconversion and Abundance of IgG Antibodies against S1-RBD of SARS-CoV-2 and Neutralizing Activity in the Chilean Population칠레 인구에서 SARS-CoV-2의 S1-RBD 및 중화 활성에 대한 IgG 항체의 혈청전환 및 풍부Clinical Trial Published on 2021-02-192022-09-10 Journal: Journal of Immunology Research [Category] MERS, 임상, 진단, 치료기술, [키워드] abundance activity age anti-RBD Anti-RBD IgG anti-SARS-CoV-2 anti-SARS-CoV-2 antibodies candidate caused Chilean clinical trial Concentration convalescent convalescent plasma correlated COVID-19 died Donor donors ELISA evaluated Gender high variability IgG majority Neutralizing activity Neutralizing antibodies neutralizing antibody offset pandemic Patient plasma Population pseudotype quantification quantified RBD IgG SARS-CoV-2 Seroconversion significant differences Symptom were used [DOI] 10.1155/2021/6680337 PMC 바로가기 [Article Type] Clinical Trial
Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)Methodology Published on 2021-02-152022-10-28 Journal: Virology Journal [Category] COVID-19, [키워드] Cell Combination condition Critical development digital PCR HIV HIV cure research human HIV immunodeficiency virus Infection Intervention priming quantification Raindance Research Result reverse transcriptases reverse transcription rhesus macaque RNA RNA samples RT-ddPCR sensitive Simian simian immunodeficiency virus SIV supplementary material target therapeutic tissue sample treatment regimen Viral reservoir [DOI] 10.1186/s12985-021-01503-5 PMC 바로가기 [Article Type] Methodology