Detection of cross-reactive immunoglobulin A against the severe acute respiratory syndrome-coronavirus-2 spike 1 subunit in saliva중증 급성 호흡기 증후군-코로나 바이러스 -2 스파이크에 대한 교차 반응성 면역 글로불린 A의 검출 1 타액의 서브 유닛Research Article Published on 2021-11-232022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] age analyzed angiotensin angiotensin converting enzyme Angiotensin converting enzyme-2 binding Biotin blood sample Blood samples breast milk Chain Reaction correlated cross-react cross-reaction cross-reactive cross-reactivity dental dentist dentists detection determined by enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay help IgG and IgM Immunity immunochromatography Immunoglobulin immunoglobulin A individual Infection involved men mucosal surfaces Mucus Patient patients PCR PCR-negative polymerase chain polymerase chain reaction Prevent proportion Protein provide purified reaction receptor Receptor-binding domain reported Saliva saliva samples SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein secretory immunoglobulin A SIgA significantly lower Spike protein subunit suppress the receptor-binding domain university virus was determined women [DOI] 10.1371/journal.pone.0249979 PMC 바로가기 [Article Type] Research Article
Saliva is superior over nasopharyngeal swab for detecting SARS-CoV2 in COVID-19 patients타액은 COVID-19 환자에서 SARS-COV2를 감지하기 위해 비 인두 면봉보다 우수합니다.Comparative Study Published on 2021-11-222022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] Admission COVID-19 patient COVID-19 patients diagnose diagnostic global pandemic Health care hospital Medical research medium mitigate Nasopharyngeal swab Non-invasive NPs pair patients Population positive RT-PCR Saliva saliva samples SARS-CoV2 Scaling significantly higher Swab tested Transport transport medium Viral Viral load viral shedding was collected [DOI] 10.1038/s41598-021-02097-2 PMC 바로가기 [Article Type] Comparative Study
Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution meltingResearch Article Published on 2021-11-222022-10-28 Journal: PLoS ONE [Category] COVID-19, SARS, [키워드] Accuracy acquisition Analysis approach bases Complete country COVID-19 COVID-19 pandemic COVID-19 testing detect diagnostic diagnostics E gene facilitated genotyping globe gold standard Internal control Laboratory melting methodology Moderate COVID-19 molecular Molecular diagnosis nasopharyngeal swabs pathogen Patient PCR PCR assay PCR products PCR testing primer protocol purine RdRP reagent reagents real time Region Resolution resulting RT-qPCR RT-qPCR assay Saliva SARS-CoV-2 SARS-CoV-2 genome sensitivity sequence serum specificity specimen specimens TaqMan targets temperature the SARS-CoV-2 genome validation viral genome [DOI] 10.1371/journal.pone.0260087 PMC 바로가기 [Article Type] Research Article
The Stability of an Isolate of the SARS-CoV-2 B.1.1.7 Lineage in Aerosols Is Similar to 3 Earlier IsolatesArticle Published on 2021-11-222022-11-16 Journal: The Journal of Infectious Diseases [Category] SARS, 변종, [키워드] absence aerosol Aerosols Affect B.1.1.7 B.1.1.7 lineage circulating COVID-19 Culture decay environmental condition examined genetic difference influence of temperature isolate Laboratory lineages lung medium persistence Relative humidity respiratory tract Saliva SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 lineage stability statistically significant difference Sunlight survival temperature the SARS-CoV-2 Transmissibility Transmission variant [DOI] 10.1093/infdis/jiab171 PMC 바로가기
Discrimination of SARS-CoV-2 Infections From Other Viral Respiratory Infections by Scent Detection DogsMedicine Published on 2021-11-182022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] aetiology canine Cell Cell culture cell culture supernatant contribute coronavirus COVID-19 demonstrated detect detection detection dog diagnostic discrimination dog double-blind study evaluate include indicate individual infected individual infected with SARS-CoV-2 Infection infections investigated mandatory non-infected cell oropharyngeal swab Other other coronavirus performed positive randomised Research respiratory Saliva SARS-CoV-2 SARS-CoV-2-infected individual Scent scent detection dog screening method screening test sensitivities sensitivity specificity Spread supernatant swab sample Symptom Testing the mean variety viral infection viral infections viral respiratory virus volatile organic compound (VOC) [DOI] 10.3389/fmed.2021.749588 PMC 바로가기 [Article Type] Medicine
Comparative analysis of loop-mediated isothermal amplification (LAMP)-based assays for rapid detection of SARS-CoV-2 genesSARS-COV-2 유전자의 빠른 검출을위한 루프-매개 등온 증폭 (LAMP)-기반 분석의 비교 분석Clinical Trial Published on 2021-11-182022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 임상, 진단, 치료기술, 치료제, [키워드] Accuracy Analysis assays Biological techniques caused changes Comparative analysis condition COVID-19 pandemic detect Detection sensitivity diagnostic diagnostics equipment exhibit False positive false positive rate False positives False-positive false-positives faster fluorescent gold gold standard group highlight identify implication in some incorporation increase infected cases isothermal amplification Kinetics Laboratory LAMP Loop-mediated isothermal amplification Microbiology techniques Nsp3 ORF1 ORF1ab point-of-care primer provide RdRP reaction time Reactions Region reported RNA copies RNA copy robust RT-LAMP RT-qPCR Saliva SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 gene sensitivity [DOI] 10.1038/s41598-021-01472-3 PMC 바로가기 [Article Type] Clinical Trial
Assessing saliva microbiome collection and processing methodsArticle Published on 2021-11-182022-10-30 Journal: NPJ Biofilms and Microbiomes [Category] COVID-19, MERS, SARS, [키워드] Accuracy Alpha Assessing Bacterial collected depleting DNA examined Health Human increase lung measure metagenomics Microbiome Next-generation sequencing progression provided reducing rRNA Saliva Sample SARS-COV-2 infection SARS-CoV-2 test second set sequence Sequencing subject subjects subset tested was performed [DOI] 10.1038/s41522-021-00254-z PMC 바로가기 [Article Type] Article
Sampling site for SARS-CoV-2 RT-PCR—An intrapatient four-site comparison from Tampere, FinlandSARS-COV-2 RT-PCR 용 샘플링 사이트-핀란드 탬퍼에서의 4 개 사이트 비교.Research Article Published on 2021-11-162022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 치료기술, [키워드] analysed anatomical Anterior can be used collected COVID-19 positive patient Cycle threshold value Cycle threshold values Diagnosis heterogeneity nasal Nasal sample nasal swab nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs nasopharynx no significant difference objective Oropharyngeal oropharynx patients positive provided recruited Result RT-PCR Saliva sampling SARS-CoV-2 SARS-CoV-2 detection significant difference situation Swab written Informed Consent [DOI] 10.1371/journal.pone.0260184 PMC 바로가기 [Article Type] Research Article
Point-of-need detection of pathogen-specific nucleic acid targets using magnetic particle spectroscopy자기 입자 분광법을 사용한 병원체 특이적 핵산 표적의 필요 시점 검출Article Published on 2021-11-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, 치료제, [키워드] addition can be used change complex Concentration COVID-19 pandemic diagnostic diagnostic test diagnostic tests diagnostics DNA experiment increase in individuals Magnetic field Magnetic nanoparticles Magnetic particle spectroscopy means medium MPS nucleic acid nucleic acid target nucleic acids pandemic pathogen pathogen-specific Pathogens Point-of-need resulting RNA Saliva SARS-CoV-2 sequence Vaccines [DOI] 10.1016/j.bios.2021.113536 PMC 바로가기 [Article Type] Article
A rapid, ultrasensitive voltammetric biosensor for determining SARS-CoV-2 spike protein in real samplesArticle Published on 2021-11-152022-11-16 Journal: Biosensors & bioelectronics [Category] 치료기술, [키워드] Accuracy acute respiratory syndrome antibody Antigen applied bovine coronavirus coronavirus disease COVID-19 demonstrated detect diagnosing early diagnosis electrochemical biosensor evaluated Interaction oropharyngeal swab pandemic polymerase chain positive Protein provide public health system RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein sensitivity serum albumin specificity Spike protein the SARS-CoV-2 Viral detection viral outbreak virus spike protein voltammetry [DOI] 10.1016/j.bios.2021.113497 PMC 바로가기