Serological identification of SARS-CoV-2 infections among children visiting a hospital during the initial Seattle outbreakArticle Published on 2020-09-012022-10-29 Journal: Nature Communications [Category] COVID-19, SARS, [키워드] Adults Care children collected COVID-19 COVID-19 case COVID-19 disease disease Epidemiology Frequency hospital identify infected children initial less MOST neutralized Neutralizing activity outbreak Paediatric research pediatric positive SARS-CoV-2 SARS-COV-2 infection Seattle Seattle Children’s Hospital seeking sera Serologically seropositive Seven The United States viral infection were infected [DOI] 10.1038/s41467-020-18178-1 PMC 바로가기 [Article Type] Article
A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopesArticle Published on 2020-08-272022-10-29 Journal: Nature Communications [Category] COVID-19, [키워드] acute phase antibody approved binding collected coronavirus SARS-CoV-2 COVID-19 pandemic described domain epitope human ACE2 receptor Human monoclonal antibody Immunotherapy in vitro inhibitory activity Isolation lymphocyte mAb multiple epitope Neutralizing Neutralizing activity neutralizing antibody neutralizing monoclonal antibody Patient RBD Receptor binding domain recognize SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike selected shown subset the antibody the disease the RBD the SARS-CoV-2 therapeutic drug therapy viral infection viral spike virus [DOI] 10.1038/s41467-020-18159-4 PMC 바로가기 [Article Type] Article
A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2Research Article Published on 2020-08-072022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome addition antibody bind binding characterized complex convalescent COVID-19 patients convalescent patient coronavirus COVID-19 defined Developing distribution epitope Epitopes exhibit host cell Local mAb mAbs membrane fusion Microscopy monoclonal antibody monomer N-terminal domain neutralization potency neutralize Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody NTD Protein protein complex pseudotyped SARS-CoV-2 RBD RBD-targeting antibody Receptor binding domain S2 domain SARS-CoV-2 Science spike the RBD the S protein the Spike the spike protein therapeutic trimeric virus [DOI] 10.1126/science.abc6952 PMC 바로가기 [Article Type] Research Article
Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primatesArticle Published on 2020-07-312022-10-29 Journal: Vaccine [Category] COVID-19, [키워드] antibody production Antibody titer CHO cell confirmed case COVID-19 COVID-19 outbreak COVID-19 patient COVID-19 vaccine death evidenced examined expressing fusion protein global pandemic immunized immunogenic induce Infection injection live SARS-CoV-2 Live virus mice MOST Neutralizing activity neutralizing response Protein Pseudovirus neutralization assay recombinant responsible SARS-CoV-2 SARS-CoV-2 spike Spike protein STABLE Strong vaccination Vaccine Vaccine development virus virus neutralizing titer [DOI] 10.1016/j.vaccine.2020.06.066 PMC 바로가기 [Article Type] Article
Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman PrimatesOriginal Article Published on 2020-07-282022-11-01 Journal: The New England Journal of Medicine [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Analysis antibody Antigen BAL BAL fluid CD4 T-cell CD8 T-cell responses changes in coronavirus coronavirus disease detectable Dilution dose eight evaluate geometric mean titer groups histopathological Inflammation inhibitory lower airway lower airways lung mRNA-1273 mRNA-1273 vaccine nasal swab Neutralizing activity pathologic performed polymerase chain Prevent primate quantification Replication response Result robust SARS-CoV-2 serum Seven specimen specimens Spike protein T-cell T-cell Response Th2 type 1 helper undetectable Vaccine vaccine group viral genome viral replication were assessed [DOI] 10.1056/NEJMoa2024671 PMC 바로가기 [Article Type] Original Article
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Research Published on 2020-07-132022-10-05 Journal: Nature Structural & Molecular Biology [Category] SARS, 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 binding antibody bind binding domain cause Cell complex coronavirus CR3022 cryo-EM crystal structure epitope fusion H11-H4 human Angiotensin-converting enzyme Influenza Interaction maturation nanobodies nanobody neutralization Neutralizing activity overlap produced RBD receptor recognize SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 Spike RBD SARS-CoV-2 virus spike trimer the spike protein viral entry [DOI] 10.1038/s41594-020-0469-6 [Article Type] Research
Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19 Research Published on 2020-07-132022-10-05 Journal: Nature Medicine [Category] 바이오마커, 변종, 임상, 치료기술, [키워드] acute respiratory syndrome angiotensin-converting enzyme 2 B cell Biomarker cellular receptor characterized circulating coronavirus coronavirus disease COVID-19 effective elicited Eliciting follicular Frequency glycoprotein hallmark helper T cell Human humoral Humoral immunity immune Immunity immunogenic membrane memory B cell Neutralizing activity neutralizing antibody neutralizing epitopes neutralizing responses pandemic Patient phenotype plasma Prevent Receptor binding domain recovered patient resolved response robust S-based vaccine S-specific antibodies SARS-CoV-2 SARS-COV-2 infection subject Vaccine Vaccine development virion with COVID-19 [DOI] 10.1038/s41591-020-0995-0 [Article Type] Research
Clinical and Analytical Performance of an Automated Serological Test That Identifies S1/S2-Neutralizing IgG in COVID-19 Patients SemiquantitativelyImmunoassays Published on 2020-06-242022-10-30 Journal: Journal of Clinical Microbiology [Category] COVID-19, MERS, [키워드] acute respiratory syndrome antibody automated average chemiluminescence CLIA clinical coronavirus CoV COVID-19 COVID-19 diagnosis Cutoff Diagnosis diagnostic diagnostics disease evaluated IgG immunoassays immunoserology Laboratory LIAISON measure Neutralization assay Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody neutralizing capacity offered pandemic performance plasma positive Predictive Protein provided ranged S1/S2 Safe SARS-CoV-2 selective sensitivity Serological testing serum specificity spike Test the SARS-CoV-2 therapeutic use to define Variation [DOI] 10.1128/JCM.01224-20 PMC 바로가기 [Article Type] Immunoassays
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization AssaysProtocol Published on 2020-05-062022-10-28 Journal: Viruses [Category] COVID-19, [키워드] 293T cell 293T-ACE2 ACE2 Affect ALAYT antibody Antibody binding assays ATCC BEI Resource Cell complement coronavirus COVID-19 cytoplasmic tail demonstrated described Express human sera Immunity infect lentiviral Lentiviral particle lentiviral pseudotype luciferase neutralization Neutralization assay Neutralizing activity Neutralizing antibodies NIH Particle Particles plasma Protein protocol pseudotype pseudotyped lentiviral particle pseudotyping reagent receptor Repository retroviral SARS-CoV-2 sera spike the SARS-CoV-2 vesicular stomatitis virus viral infection viral particle virus VSV [DOI] 10.3390/v12050513 PMC 바로가기 [Article Type] Protocol
Characterization of novel monoclonal antibodies against MERS-coronavirus spike proteinArticle Published on 2020-01-132022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] amino acid substitution antibody binding affinity cause characterization chimeric coronavirus Critical develop domain East elicit epitope FIVE generate glycoprotein host cells identification immunization immunized lentivirus mAb mAbs MERS-CoV mice middle monoclonal antibody Mortality Mutation neutralization Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody neutralizing effect not neutralize Protein Proteins pseudotyped pseudovirus Pulmonary infection RBD residue respiratory selected specific mutation spike Spike protein subunit Vaccine variant viral entry virus wild type [DOI] 10.1016/j.virusres.2020.197863 PMC 바로가기 [Article Type] Article