Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged MiceArticle Published on 2021-03-292022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] activity acute respiratory syndrome adjuvant aged mice antibodies approved B cell cellular immunity coronavirus domain ectodomain Effect effective Efficacy Emergency use full-length globe High dose humans IgG antibody IgG1 IgG2a immune responses immunogenic immunogenicity information inhibiting interferon-γ isotypes low dose mice Neutralizing Neutralizing activity pandemic disease RBD-hACE2 inhibition Receptor binding domain Result S1 and S2 SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike-specific SARS-CoV-2 vaccine secreting spike subunit T cell the S1 subunit vaccination Vaccine vaccine antigen vaccine candidate vaccine dose [DOI] 10.3390/vaccines9040316 PMC 바로가기 [Article Type] Article
Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region모바일 연구소 B-LiFE에 의한 COVID-19 진단에서 IgM 및 IgG 검출의 진단적 가치: 피에몬테 지역의 대규모 테스트 전략Article Published on 2021-03-242022-09-10 Journal: International Journal of Environmental Research an [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute infectious disease age Analysis antibodies asymptomatic individual Asymptomatic individuals Care caused Cohort consequence coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 case COVID-19 cases COVID-19 outbreak detect detection detection rate Diagnosis domain emerging biological threats first responders Health Health care help IgG IgM and IgG individual Infectious disease Italy Laboratory Large-scale testing massive Mobile moderate Novel coronavirus nucleocapsid protein performed Piedmont police positively correlated Prevalence professionals Protein Public Health Preparedness RBD protein Region residence responders S-RBD SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 NP antibody SARS-CoV-2 spike SARS-CoV-2 spike receptor-binding domain Seroconversion seroconverted Serological testing Seroprevalence serum serum antibodies serum antibody Sex Strategy Surveillance Testing the SARS-CoV-2 value volunteers Whole blood [DOI] 10.3390/ijerph18073372 PMC 바로가기 [Article Type] Article
Soluble Spike DNA Vaccine Provides Long-Term Protective Immunity against SARS-CoV-2 in Mice and Nonhuman PrimatesArticle Published on 2021-03-242022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] Antibody Response CD4+ CD8+ T cell COVID-19 detectable DNA DNA vaccine dose-dependent manner elicited exhibited immunization immunized mice multifunctional Neutralizing antibody response primate protective immune response provide reduced viral load responses SARS-CoV-2 SARS-CoV-2 spike seroconverted spike Spread Support T cell response vaccination Vaccine vaccine candidate [DOI] 10.3390/vaccines9040307 PMC 바로가기 [Article Type] Article
A safe and highly efficacious measles virus-based vaccine expressing SARS-CoV-2 stabilized prefusion spikeSARS-CoV-2 안정화된 융합 전 스파이크를 발현하는 안전하고 효과적인 홍역 바이러스 기반 백신423 Published on 2021-03-232022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 animal animals attenuated vaccine backbone body weight Body weight loss caused children Complete convalescent sera coronavirus Cotton rat COVID-19 patient COVID-19 patients cytokine Cytokine storm develop Efficacy evaluated expressing form full-length S full-length S protein Genome hamster hamsters highlight IFNAR Immunity immunized induce lung Lung pathology Lungs measles virus measles virus vector mice nasal turbinates Neutralizing antibodies neutralizing antibody pandemic of COVID-19 prefusion prefusion S prefusion spike produced Protein provided RBD Receptor binding domain Replication respiratory S antigen Safe SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 spike SARS-CoV-2 vaccine severe acute respiratory syndrome Coronavirus Significance single dose T cell T cell response the SARS-CoV-2 These data triggering Vaccine vaccine candidate vaccine strain virus weight loss [DOI] 10.1073/pnas.2026153118 PMC 바로가기 [Article Type] 423
DNA vaccine candidate encoding SARS-CoV-2 spike proteins elicited potent humoral and Th1 cell-mediated immune responses in miceSARS-CoV-2 스파이크 단백질을 인코딩하는 DNA 백신 후보는 마우스에서 강력한 체액성 및 Th1 세포 매개 면역 반응을 유발했습니다Research Article Published on 2021-03-222022-08-13 Journal: PLoS ONE [Category] Biochemistry, COVID-19, SARS, [키워드] ACE2 Antigen balance binding breadth candidate cell-mediated immune response COVID-19 Critical died DNA vaccine DNA vaccines Effective vaccines elicited expressing followed by full length full-length S geometric mean geometric mean titer geometric mean titers GMTs highest humoral IFN-γ IgG IgG1 IgG2a infection with SARS-CoV-2 inhibit Interaction limit lowest magnitude mice neutralizing antibody pandemic peptide positive cell positive cells PRNT50 PRNT50 titers Protein robust SARS-CoV-2 spike SARS-CoV-2 spike peptide SARS-CoV-2 spike protein sera splenocyte splenocytes Stimulation T cell T cell responses T cells Th1 Vaccine Viral viral RBD wild type [DOI] 10.1371/journal.pone.0248007 PMC 바로가기 [Article Type] Research Article
Shedding Light on the Inhibitory Mechanisms of SARS-CoV-1/CoV-2 Spike Proteins by ACE2-Designed PeptidesACE2 설계 펩티드에 의한 SARS-CoV-1/CoV-2 스파이크 단백질의 억제 메커니즘에 대한 조명Article Published on 2021-03-222022-09-11 Journal: Journal of Chemical Information and Modeling [Category] MERS, SARS, 신약개발, 치료제, [키워드] ACE2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 binding binding affinities binding affinity can be used cellular receptor comparable Contact coronavirus COVID-19 COVID-19 pandemic demonstrated domain domains Efficiency enzyme full-length Host host cells human ACE2 Interaction involved kinetic analysis LIGHT mechanism neutralization novel peptide peptides Previous studies previous study protocol responsible route SARS Coronavirus SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 spike sequence shedding shown spike Spike protein Spike proteins surface protein the spike protein thermodynamic virus entry virus infection [DOI] 10.1021/acs.jcim.0c01320 PMC 바로가기 [Article Type] Article
Deletion of the SARS-CoV-2 Spike Cytoplasmic Tail Increases Infectivity in Pseudovirus Neutralization AssaysVaccines and Antiviral Agents Published on 2021-03-162022-10-30 Journal: Journal of Virology [Category] COVID-19, [키워드] acute respiratory syndrome amino acids antibody Antibody therapeutics approach Biosafety Level 3 BSL2 BSL3 Cell Characteristics coronavirus coronavirus disease COVID-19 COVID-19 pandemic Critical cytoplasmic tail defined Deletion facilitate full-length generate hACE2 human ACE2 Humoral immunity IgG increase Infectivity inhibitor lentiviral Lentiviral vector limit of detection log murine Neutralization assay Neutralizing antibodies pandemic Precision produced pseudovirion pseudovirus Pseudovirus neutralization assay purified SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 spike SARS-CoV-2 variant sensitivity sera spike Spread tail target cells the SARS-CoV-2 vaccination Vaccine variant viral entry viral pathogen virulent virus virus entry [DOI] 10.1128/JVI.00044-21 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Accurate point-of-care serology tests for COVID-19COVID-19에 대한 정확한 현장 진료 혈청 검사Research Article Published on 2021-03-162022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, 치료법, [키워드] antibody antibody assay Antibody testing Antigen Blood CLIA correlated COVID-19 COVID-19 antibody COVID-19 infection COVID-19 vaccine COVID-19 vaccines detectable determine effective enrolled evaluated feasible Hospitalization identify immunoassay IMPROVE individual individuals with COVID-19 Infection lateral flow mechanical ventilation nucleocapsid nucleocapsid protein outcome PCR PCR-positive performed POC point-of-care positive PCR produced required Result resulting SARS-CoV-2 spike Screen screened sensitivity serology test serum specificity subject subjects symptomatic the immunoassay vaccine response variable virus [DOI] 10.1371/journal.pone.0248729 PMC 바로가기 [Article Type] Research Article
Bat and pangolin coronavirus spike glycoprotein structures provide insights into SARS-CoV-2 evolutionArticle Published on 2021-03-112022-10-04 Journal: Nature Communications [Category] SARS, 변종, [키워드] activity bat binding cell entry cell membranes cellular receptor comparable conformational coronavirus Coronaviruses Critical Cross-species transmission cryo-EM structure Cryoelectron microscopy ectodomain electron microscopy Evolution Evolution of SARS-CoV-2 glycoprotein human ACE2 receptor implication inactive Infection pseudovirus RaTG13 RBD RBDs Receptor-binding domain recognizing required residue SARS-CoV-2 SARS-CoV-2 evolution SARS-CoV-2 spike spike spike glycoprotein spike trimer spikes Structure the RBD the SARS-CoV-2 Viral protein virus [DOI] 10.1038/s41467-021-21767-3 PMC 바로가기 [Article Type] Article
SARS-CoV-2-directed antibodies persist for more than six months in a cohort with mild to moderate COVID-19SARS-CoV-2 유도 항체는 경증에서 중등도의 COVID-19 코호트에서 6개월 이상 지속됩니다.Original Paper Published on 2021-03-102022-09-10 Journal: Infection [Category] MERS, SARS, 진단, 치료기술, [키워드] age anti-SARS-CoV-2 anti-SARS-CoV-2 antibody antibodies antibody Antibody titer Antibody titers association Cohort COVID-19 detectable disease severity ELISA Follow-up healthcare worker identify IgA IgG IgG antibody IgG response IgM IgM antibodies IgM antibody IgM response IgM responses immune response immune responses increasing age individual inter-individual less Mild Moderate COVID-19 mounted objective onset of symptoms parameter participant PCR-confirmed COVID-19 positive Questionnaires Result robust S protein SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein serological Serological immune response Severity of disease Spike protein subject supplementary material Symptom Symptoms Variability variations were assessed were measured [DOI] 10.1007/s15010-021-01598-6 PMC 바로가기 [Article Type] Original Paper