Biofilm-associated toxin and extracellular protease cooperatively suppress competitors in Bacillus subtilis biofilms생물막 관련 독소 및 세포외 프로테아제는 Bacillus subtilis 생물막에서 경쟁자를 협력적으로 억제합니다Research Article Published on 2019-10-172022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] aggregate Alter antibiotic Antibiotics Antimicrobials assistance B . subtilis Bacillus subtilis Bacteria Bacterial bacterial composition biofilm Cell cells combating contribute Defense DEGs demonstrated diffusion Diseases effective eliminated excluded expression Extracellular food contamination growth impair Infectious diseases inhibit leads medium multicellular mutant mutations Neutral observation overexpression penetrate peptide Polymer polymers Prevent problem problems protease PROTECT Protein Regulatory required resistance gene resistance genes secreted solid medium Space suppress Taking Tolerance Treatment type strain upstream wild-type strain [DOI] 10.1371/journal.pgen.1008232 PMC 바로가기 [Article Type] Research Article
Expression of a novel class of bacterial Ig-like proteins is required for IncHI plasmid conjugationIncHI 플라스미드 접합을 위해서는 새로운 종류의 세균성 Ig 유사 단백질의 발현이 필요합니다Research Article Published on 2019-09-172022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] (colistin Antibiotic resistance Antibiotics antimicrobial resistance Bacterial bacterial cell bacterial cells Bacterial infections caused Cell cell motility cells cellular cellular structure coding colistin Contact contribute Critical determinants dissemination DNA domain enterobacteria Enterobacteriaceae Europe Express expression facilitate flagella group Infection instances interact Interaction involved liquid medium mating pair medium multidrug-resistant occur ORF Plasmid plasmids Population Protein reduce reported required restrict retained Salmonella secretion significantly Spread target the cell transfer Transmission Treatment widespread [DOI] 10.1371/journal.pgen.1008399 PMC 바로가기 [Article Type] Research Article
Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling PigletsImmunology Published on 2019-04-242022-11-01 Journal: Frontiers in Immunology [Category] Coronavirus, MERS, [키워드] acute diarrhea Affect affected antibody antibody secreting cell ASC B cells cause change circulating Control correlated Course Critical Cytokines determine during pregnancy epidemic diarrhea example gestation gut-mammary-secretory IgA axis IgA IgG immature immune system immune parameter immune protection immune response Immunity Impact implication Infection influence inoculated intestinal lactogenic Lactogenic immunity lymphocyte Maternal medium mucosal naïve Neonatal Neonate Neutralizing PEDV positively correlated Pregnancy pregnant protection required response resulting serum significantly significantly higher Stage stimulate stimulated subsequent Suckling survival survival rate swine three group Vaccines viral infections virulence virus [DOI] 10.3389/fimmu.2019.00727 PMC 바로가기 [Article Type] Immunology
Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay KitsResearch Article Published on 2019-03-132022-10-30 Journal: mSphere [Category] Coronavirus, MERS, [키워드] Accuracy Affect Analysis antibodies antibody Antigen blocking collected comparison Conventional cross-reactivity Culture current diagnostic early stage ELISA ELISA kit ELISAs enzyme-linked immunosorbent assay evaluated False positive gastroenteritis include individual infections inoculated measure medium Miller observation period porcine respiratory coronavirus positive Prevent proportion quarantine reported Research respiratory Respiratory Coronavirus S protein sensitivity serologic serological test serology serum serum antibodies serum sample specificity swine TGEV transmissible gastroenteritis virus [DOI] 10.1128/mSphere.00017-19 PMC 바로가기 [Article Type] Research Article
Genome-wide CRISPR-dCas9 screens in E . coli identify essential genes and phage host factorsE의 게놈 전체 CRISPR-dCas9 스크린. 대장균은 필수 유전자와 파지 숙주 인자를 식별합니다.Research Article Published on 2018-11-072022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] addition applied Bacteria Bacterial bacterial capsule bacterial gene bacterial genes bacteriophage bacteriophages capsids Cas9 Cas9 RNA-guided nuclease catalytic Cell chromosome colanic acid convenience CRISPR CRISPR-Ca CRISPR-dCas9 screen CRISPRi dCas9 dCas9 protein detect E . coli expression level Factor functional Gene Expression Genes Genetic genetic interactions genetic screens Genome genomes growth guide RNA High-throughput genetic screen High-throughput genetic screens highlight highlighting Host identify infection process Interaction involved kill library mechanism medium modulate mutant over pathway phage phage λ phages phages λ phenotype Phenotypes Plasmid powerful tool predict progeny random remained reported required resistance mechanism Screen sgRNA sgRNAs technique tested transduction assay utility [DOI] 10.1371/journal.pgen.1007749 PMC 바로가기 [Article Type] Research Article
Cyclic di-AMP regulation of osmotic homeostasis is essential in Group B Streptococcus삼투성 항상성의 순환 di-AMP 조절은 B군 연쇄상 구균에서 필수적입니다Research Article Published on 2018-04-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] absence accumulating accumulation assumed Bacteria Bacterial betaine bind binding cellular cellular physiology compensatory mutations conditions conserved Control cyclic dacA Deletion demonstrated dependent on enzyme etiological agent expression function functional glycine glycine betaine group growth homeostasis host cell host cells host-pathogen interactions hyperosmotic identification identify Infection involved leads Loss loss-of-function loss-of-function mutation maintain mechanism medium Meningitis monophosphate mutant Mutation Neonatal nucleotide nucleotides opportunistic pathogen osmolyte osmoregulation osmotic osmotic regulation pathogen pathway phenotype Potassium predominant promoter Protein regulate Regulation second messenger second messengers secondary messenger septicaemia Streptococcus Streptococcus agalactiae susceptibility Transcription transcriptional viability [DOI] 10.1371/journal.pgen.1007342 PMC 바로가기 [Article Type] Research Article
A two-step transport pathway allows the mother cell to nurture the developing spore in Bacillus subtilis2단계 수송 경로를 통해 모세포가 Bacillus subtilis에서 발달 중인 포자를 양육할 수 있습니다.Research Article Published on 2017-09-252022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] accumulate accumulation Bacillus anthracis Bacillus subtilis Bacteria Bacterial Cell Clostridium difficile complex Concentration conserved Culture culture medium dehydration desiccation dipicolinic acid displace dormancy dormant dry weight engulfment enzyme Enzymes Evidence Express Factor forespore hallmark hallmarks homolog homologs idea late stage mechanism medium membrane membranes molecular basis Morphological mother mother cell Mutagenesis non-pathogenic bacteria nucleoside pathogenic pathway predicted produced Protective Protein pyridine-2,6-dicarboxylic acid remained required residue residues small molecule spore spore core spores sporulation Support translocated Transport vegetative growth water [DOI] 10.1371/journal.pgen.1007015 PMC 바로가기 [Article Type] Research Article
Evaluation of biosecurity measures to prevent indirect transmission of porcine epidemic diarrhea virusResearch Article Published on 2017-04-052022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] addition Animal movement biosecurity conditions effective Effectiveness elevated epidemic diarrhea evaluate Farm personnel fomites group Indirect transmission measure medium mitigate occur peaked PEDV Personal protective equipment polymerase chain Porcine epidemic diarrhea virus positive post infection PPE Prevent protocol rectal swab remained Result reverse transcription risk RNA rRT-PCR sentinel Swab Transmission Trial Viral RNA virus Virus shedding [DOI] 10.1186/s12917-017-1017-4 PMC 바로가기 [Article Type] Research Article
Cellular entry of the porcine epidemic diarrhea virusArticle Published on 2016-06-152022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Americas aminopeptidase N Asia binding binding activity carbohydrate Cell coronavirus Culture determine diarrhea domain East epidemic diarrhea epithelial cell Factor functional glycoprotein host cell in vitro in vivo Infection initial Interaction medium membrane fusion N-terminal observation outbreak PED PEDV Porcine epidemic diarrhea virus protease Protein proteolytic activation receptor receptor interaction replicate reported resulting S protein S proteins sialic acid Sialic acid binding small intestine spike subsequent target cell the S protein tissue tropism trimer villus virus virus entry virus infection virus tropism [DOI] 10.1016/j.virusres.2016.05.031 PMC 바로가기 [Article Type] Article
Evaluation of serological cross-reactivity and cross-neutralization between the United States porcine epidemic diarrhea virus prototype and S-INDEL-variant strainsResearch Article Published on 2016-04-052022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] antibody antibody assay Antisera Cell Cell culture collected cross-neutralization cross-reactivity Culture detect detection rate determine diagnostic ELISA epidemic diarrhea evaluated examined FIVE fluorescent generate group IFA in vitro infecting inoculated lab Laboratory medium neutralized offered PEDV Porcine epidemic diarrhea virus positive post inoculation prototype Result S-INDEL serological Serological assay serum sample strain Strains the antibody the United State variant virus Virus neutralization viruses [DOI] 10.1186/s12917-016-0697-5 PMC 바로가기 [Article Type] Research Article