Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assayLuminex 비드 기반 분석을 사용하여 중증 및 경증 COVID-19 사례에서 항체 검출을 위한 표적으로 SARS-CoV-2 스파이크 및 뉴클레오캡시드 단백질 평가Article Published on 2021-02-012022-09-11 Journal: Journal of virological methods [Category] MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibody Antibody detection Antibody test Antigen assays Bead-based assay Combination coronavirus COVID-19 case domain ELISA Evaluating FIVE IgA antibodies IgA antibody IgA response IgG IgG antibody IgM include Infection Luminex antibody test Mild mild case mild cases multiple antigen multiple antigens Multiplexing neutralization neutralization test neutralizing antibody nucleocapsid nucleocapsid protein offer Pathogens RBD RDTs Receptor-binding domain Recombinant nucleocapsid protein respiratory SARS-CoV-2 SARS-CoV-2 spike sera serological serological test Serological tests Serosurveillance severe cases significantly lower specificity Support symptom onset target threshold titers virus neutralization assays virus neutralization test while [DOI] 10.1016/j.jviromet.2020.114025 PMC 바로가기 [Article Type] Article
Antibody dynamics to SARS-CoV-2 in asymptomatic COVID-19 infections무증상 COVID-19 감염에서 SARS-CoV-2에 대한 항체 역학Article Published on 2021-02-012022-09-12 Journal: Allergy [Category] MERS, SARS, 진단, [키워드] analyzed antibodies antibody Antibody dynamics Asymptomatic Asymptomatic carrier Asymptomatic COVID-19 asymptomatic individual asymptomatic infections Biomarker Combination condition controls COVID-19 demonstrated Detection sensitivity Diagnosis diagnostic did not produce dynamics of antibody Enrollment epidemiologically healthy individual Humoral immunity IgM and IgG IgM antibody IgM responses immunization strategies implication individual Infection infections knowledge Microarray microarray analysis mild patient N protein Neutralization assay neutralizing antibody nucleic acid pandemic peaked produced profile Protein proteome Pseudotyped virus public health raise recruited SARS-CoV-2 SARS-CoV-2 proteome screened sensitivity serological serological survey Serological testing serum significantly symptoms onset the NAT [DOI] 10.1111/all.14622 PMC 바로가기 [Article Type] Article
More Than Just Gene Therapy Vectors: Lentiviral Vector Pseudotypes for Serological Investigation유전자 치료 벡터 그 이상: 혈청학적 조사를 위한 Lentiviral Vector PseudotypesReview Published on 2021-01-312022-09-01 Journal: Viruses [Category] MERS, 진단, 치료기술, 치료제, [키워드] Above Analysis Anti-viral Cell culture cell entry characterization COVID-19 pandemic culture system envelope envelope protein evaluate flexibility handling immune response immune responses Infection investigation landscape lentiviral lentiviral vectors lentivirus Neutralising Antibodies neutralising antibody Outbreaks Protein pseudotype pseudotyped sequence serological Serological assay serological assays serology shown speed Surrogates target vaccination Vaccines vector Viral viral surface virus viruses [DOI] 10.3390/v13020217 PMC 바로가기 [Article Type] Review
SARS-CoV-2 infection elicits a rapid neutralizing antibody response that correlates with disease severitySARS-CoV-2 감염은 질병 중증도와 상관관계가 있는 신속한 중화 항체 반응을 유발합니다Article Published on 2021-01-282022-09-01 Journal: Scientific Reports [Category] 진단, 치료제, [키워드] Adaptive immunity age Analysis analyzed antibody appearance association Asymptomatic individuals Cohort COVID-19 defined dependence Detection limit diagnosed disease severity elicit enrolled Gender Hospitalized hospitalized patients Humoral immunity identify immune mechanism immunology individual Infectious diseases Kinetics longitudinal longitudinal analysis magnitude Multiple Multiple regression Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody responses neutralizing response Neutralizing titer neutralizing titers non-parametric test non-parametric tests parameters participant Particles protective effect reached replicative SARS-CoV-2 infected individual SARS-CoV-2 infected individuals SARS-COV-2 infection serological significantly increased symptoms onset These data titers Treatment virological tests virus were assessed were used [DOI] 10.1038/s41598-021-81862-9 PMC 바로가기 [Article Type] Article
Systematic evaluation of IgG responses to SARS-CoV-2 spike protein-derived peptides for monitoring COVID-19 patients코로나19 환자 모니터링을 위한 SARS-CoV-2 스파이크 단백질 유래 펩타이드에 대한 IgG 반응의 체계적인 평가Clinical Trial Published on 2021-01-222022-08-13 Journal: Cellular and Molecular Immunology [Category] SARS, 임상, 진단, 치료기술, [키워드] 12-mer peptide 95% CI a peptide microarray aa 1148–1159 aa 553–564 aa 577–588 aa 601–612 aa 625–636 Adaptive immunity analyzed antibody asymptomatic patient clinical centers Cohort collected combating Combination comparable Control Coverage COVID-19 cases COVID-19 diagnosis COVID-19 pandemic COVID-19 patient COVID-19 patients cross-reactivity Diagnosis diagnostic Diagnostic markers ELISA exhibited human coronavirus IgG IgG response independent other coronavirus other coronaviruses Patient peptide peptide microarray peptides reagent recombinant Recombinant spike protein S protein S1 protein S1–101 S1–105 S1–93 S1–97 S2–78 SARS-CoV-2 SARS-CoV-2 spike sensitivity sera serological serological test Serological tests several S protein-derived 12-mer peptides specificity spike Spike protein symptomatic and asymptomatic the S1 protein [DOI] 10.1038/s41423-020-00612-5 PMC 바로가기 [Article Type] Clinical Trial
Comparison of pre-analytical characteristics for molecular and serological diagnostics of COVID-19Article Published on 2021-01-192022-11-01 Journal: GMS Hygiene and Infection Control [Category] COVID-19, [키워드] addition Amy analyzed Brushing Characteristics comparable Concordance concordance rate conducted correlation COVID-19 COVID-19 patients diagnosis of SARS-CoV-2 diagnostic diagnostics early stage healthcare worker hospitalized patient Infection less medium molecular nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs no difference Patient PCR performed pharyngeal pharyngeal lavage positive patient positive patients positive result Predictive RT-PCR Saliva SARS-CoV-2 SARS-COV-2 infection serological specimen Swab swabs Transmission [DOI] 10.3205/dgkh000374 PMC 바로가기 [Article Type] Article
An Overview of Nucleic Acid Testing for the Novel Coronavirus SARS-CoV-2Medicine Published on 2021-01-182022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] Accuracy acid acute respiratory syndrome affected Analysis Clinical diagnosis Clinicians coronavirus COVID-19 Diagnosis diagnostic early diagnosis effort false-negative novel nucleic acid nucleic acid testing pandemic performed problem progression qRT-PCR reducing SARS-CoV-2 SARS-CoV-2 nucleic acid serological specimen Support Testing the disease therapeutic efficacy Treatment [DOI] 10.3389/fmed.2020.571709 PMC 바로가기 [Article Type] Medicine
Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19COVID-19를 가진 무증상 및 증상이 있는 개인에서 총 항-스파이크 항체 및 이소타입 전환의 민감한 감지Article Published on 2021-01-162022-09-10 Journal: Cell Reports Medicine [Category] MERS, 진단, 치료기술, [키워드] acquisition acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibodies antibody antibody levels Antibody Response Asymptomatic asymptomatic individual Asymptomatic individuals asymptomatic infection asymptomatic infections can be used coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Cytometry detect develop dominant Early detection Flow flow cytometry IgA IgG IgG subclasses IgG1 IgM Immunoglobulin immunoglobulin M individual individuals with COVID-19 Infection infections isotype switching limit plasma sample plasma samples Protein respiratory S protein SARS-CoV-2 sensitive serological serological analysis severe acute respiratory syndrome Coronavirus significantly higher specific antibodies specific antibody specific IgM Spread symptomatic symptomatic and asymptomatic symptomatic individual symptomatic individuals with COVID-19 [DOI] 10.1016/j.xcrm.2021.100193 PMC 바로가기 [Article Type] Article
Impact of SARS-CoV-2 pandemic among health care workers in a secondary teaching hospital in Spain스페인의 중등 교육 병원에서 의료 종사자들 사이에 SARS-CoV-2 대유행의 영향Research Article Published on 2021-01-142022-09-10 Journal: PLoS ONE [Category] MERS, 치료기술, [키워드] age appearance assigned carried category compatible symptom compatible symptoms continuous surveillance COVID-19 COVID-19 pandemic cross-sectional cumulative diagnosis of SARS-CoV-2 doctor group HCW Health Health care worker Health personnel healthcare Healthcare system Healthcare systems healthcare worker Healthcare workers highest hospital identify IgG Impact incidence increased risk individual Infection medical departments medical service medical services medical staff molecular occupational occupational health organizations Patient positive presenting symptom presenting symptoms Prevalence recruited risk rRT-PCR SARS-CoV-2 SARS-CoV-2 IgG SARS-COV-2 infection SARS-CoV-2 pandemic serological service Spain Surveillance Symptoms Viral virus women [DOI] 10.1371/journal.pone.0245001 PMC 바로가기 [Article Type] Research Article
Laboratory-Based Resources for COVID-19 Diagnostics: Traditional Tools and Novel Technologies. A Perspective of Personalized MedicineReview Published on 2021-01-132022-10-30 Journal: Journal of Personalized Medicine [Category] COVID-19, [키워드] Accuracy antibody Asymptomatic carried category cause caused Clinical use Combination contagious Coronavirus infection COVID-19 COVID-19 in patients COVID-19 pandemic dependent on Diagnosis diagnostic diagnostics Efficiency Enzyme-linked immunosorbent assay (ELISA) Factors Final global healthcare healthcare Healthcare system identify increase increase in infected patient Infection isothermal amplification Laboratory lateral flow immunoassay Lateral flow immunoassay (LFIA) LFIA limitations Loop mediated isothermal amplification (LAMP) Medicine Mild novel pandemic patients patients with COVID-19 Personalized medicine platform polymerase chain population immunity promising biomarkers repeated Research resources retrospective reverse transcription Reverse transcription polymerase chain reaction (RT-PCR) RT-PCR SARS-CoV-2 SARS-CoV-2 virus Sensitivity and specificity serological severity Spread spread of COVID-19 susceptibility the disease the SARS-CoV-2 the timing Traditional Treatment Variation widespread with COVID-19 [DOI] 10.3390/jpm11010042 PMC 바로가기 [Article Type] Review