Multiplex PCR – Page 5 – Communicable Diseases Research

Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance

긴급 SARS-CoV-2 우려 변종: 신속한 탐지 및 감시를 위한 새로운 멀티플렉스 실시간 RT-PCR 분석

Research Article

Published on 2022-02-232022-09-10
Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 변종, 치료기술, 치료제,

Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR

다중 실시간 PCR로 스파이크 단백질의 단일 염기 다형성을 식별하여 우려되는 SARS-CoV-2 변이체 감시

Article

Published on 2022-02-012022-09-11
Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술,

Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and emerging variants

SARS-CoV-2 대유행 모니터링: 확립된 변이체 및 신규 변이체의 신속한 식별을 위한 단일 염기 다형성 검출을 통한 스크리닝 알고리즘

Article

Published on 2022-01-012022-09-11
Journal: Clinical microbiology and infection : the official [Category] SARS, 변종, 치료기술, 치료제,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Algorithm Alpha applied assessment Beta Chain Reaction Concordance coronavirus coronavirus disease Coronavirus disease 2019 Delta determine disease E484K Epsilon Eta evaluate evaluated failure flexible Gamma gene mutations H655Y identify include L452R Lineage Local melting melting curve analysis monitoring Multiplex PCR N501Y nucleic acid testing P681R pangolin Pangolin lineage PCR performed polymerase chain reaction polymorphism positive sample S-gene mutation S-gene mutations SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 viral RNA Sequencing Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Severe acute respiratory syndrome coronavirus 2 variants single nucleotide Single nucleotide polymorphism SNP SNPs target Testing Testing algorithm the SARS-CoV-2 variant variants Viral RNA was performed WGS whole-genome sequencing
[DOI] 10.1016/j.cmi.2021.09.007 PMC 바로가기 [Article Type] Article

SARS-CoV-2 detection in wastewater using multiplex quantitative PCR

다중 정량 PCR을 사용한 폐수 내 SARS-CoV-2 검출

Article

Published on 2021-11-252022-09-11
Journal: The Science of the total environment [Category] SARS, 치료기술, 치료법, 치료제,

A Novel Strategy for the Detection of SARS-CoV-2 Variants Based on Multiplex PCR-Mass Spectrometry Minisequencing Technology

다중 PCR-질량 분석 미니시퀀싱 기술을 기반으로 하는 SARS-CoV-2 변이체 감지를 위한 새로운 전략

Research Article

Published on 2021-11-172022-09-10
Journal: Microbiology Spectrum [Category] MERS, SARS, 변종, 진단, 치료기술,

[키워드] (alpha (Alpha) (Beta) Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Alpha B.1.1.7 B.1.351 B.1.429 B.1.526 B.1.617.2 Bacteria Beta can be used can not coronavirus COVID-19 pandemic COVID-19 patient COVID-19 patients cycle threshold D614G Delta detect detection Detection limit E484K Epsilon expected extension Gamma identify Infection Iota K417N key factor L452R less mass spectrometry mPCR-MS minisequencing multiplex Multiplex PCR multiplex PCR amplification multisite mutated Mutation mutation type N501Y new SARS-CoV-2 non-COVID-19 patient Non-COVID-19 patients novel nucleic acid nucleic acid sequence open P.1 P681H P681R pathogen Pathogens PCR PCR amplification Plasmid positive sample probe provide qPCR quantitative PCR Real-time PCR Receptor-binding domain respiratory respiratory tract respiratory viruses SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant SARS-CoV-2 variants screening and identification second wave selected sequence sequencing technology severe acute respiratory syndrome Coronavirus SNPs specificity Spectrometry Spike protein Spread Strategy technique technology the receptor-binding domain the spike protein time-consuming variant variants were used whole-genome sequencing
[DOI] 10.1128/Spectrum.01267-21 PMC 바로가기 [Article Type] Research Article

Interlaboratory comparison of SARS-CoV2 molecular detection assays in use by U.S. veterinary diagnostic laboratories

미국 수의 진단 연구소에서 사용 중인 SARS-CoV2 분자 검출 분석의 연구소 간 비교

Article

Published on 2021-11-012022-09-11
Journal: Journal of veterinary diagnostic investigation : o [Category] SARS, 치료기술,

[키워드] affected analyzed buffer CDC assay CDC assays clinical sample clinical samples collaboration Critical Ct values diagnostic evaluate evaluated expected extraction Factors help Host indicated interlaboratory comparison Laboratory limit of detection medium molecular Multiplex PCR National organization organizations outbreak PCR reaction Probability protocols Qualitative Quantitative quantitative assessment reaction real-time RT-PCR reported reproducibility reservoir Result RNA RNA sample RT-PCR assay sample collection SARS-CoV2 SARS-CoV2 detection sensitivity Standard deviation Standardization Surveillance TE buffer Transport transport medium tris was performed
[DOI] 10.1177/10406387211029913 PMC 바로가기 [Article Type] Article

Evaluation of a multiplex PCR screening approach to identify community-acquired bacterial co-infections in COVID-19: a multicenter prospective cohort study of the German competence network of community-acquired pneumonia (CAPNETZ)

COVID-19에서 지역사회 획득 세균 동시 감염을 식별하기 위한 다중 PCR 스크리닝 접근 방식 평가: 지역사회 획득 폐렴의 독일 역량 네트워크(CAPNETZ)에 대한 다기관 전향적 코호트 연구

Observational Study

Published on 2021-10-232022-09-10
Journal: Infection [Category] MERS, SARS, 임상, 치료기술,

[키워드] Admission analyzed antibiotic stewardship Antibiotic treatment approach bacterial co-infection bacterial pathogen Bacterial pathogens Chain Reaction clinical Co-infection Cohort Community-acquired co-infection community-acquired pneumonia competence coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 patients early phase evaluate facilitate Frequency Germany Haemophilus Haemophilus influenzae Hospital admission Hospitalization hospitalized COVID-19 patient hospitalized COVID-19 patients ICU ICU admission identify inflammation markers knowledge Legionella pneumophila Moraxella catarrhalis Mortality mortality rate Most patient Most patients multicenter multiplex Multiplex PCR Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nasopharyngeal swabs network no correlation on admission pathogen detection Pathogens Patient PCR Pneumonia polymerase chain polymerase chain reaction positive PCR positive PCR result prospective cohort study purulent sputum Rapid multiplex PCR diagnostic recruited Result reverse transcription risk Risk assessment significantly sputum Staphylococcus aureus Streptococcus pneumoniae supplementary material Switzerland Treatment Treatment strategies treatment strategy
[DOI] 10.1007/s15010-021-01720-8 PMC 바로가기 [Article Type] Observational Study

Respiratory Infections in Children During a Covid-19 Pandemic Winter

Pediatrics

Published on 2021-10-182022-10-31
Journal: Frontiers in Pediatrics [Category] COVID-19,

Comparison of 11 respiratory pathogens among hospitalized children before and during the COVID-19 epidemic in Shenzhen, China

중국 심천의 Covid-19 전염병 전과 중에 입원 어린이들 사이에서 11 개의 호흡기 병원체의 비교

Comparative Study

Published on 2021-10-092022-09-10
Journal: Virology Journal [Category] MERS, SARS, 치료기술,

[키워드] Acute respiratory infection acute respiratory infections Adenovirus analyzed children China circulation collected COVID-19 COVID-19 epidemic detection rate enrolled Epidemic Hospitalized Human human metapneumovirus human parainfluenza virus Human respiratory syncytial virus Human rhinovirus Influenza influenza A measure Multiplex PCR Mycoplasma pneumoniae nasopharyngeal swabs outbreak parainfluenza virus pathogen Pathogens Patient pneumoniae Prevalence preventive measure preventive measures remains uncertain respiratory respiratory pathogen Respiratory pathogens respiratory syncytial virus respiratory tract infection Result rhinovirus SARS-CoV-2 Seasonal influenza Shenzhen significantly lower Swab virus
[DOI] 10.1186/s12985-021-01669-y PMC 바로가기 [Article Type] Comparative Study

Impact of rapid multiplex PCR on management of antibiotic therapy in COVID-19-positive patients hospitalized in intensive care unit

Observational Study

Published on 2021-10-012022-10-05
Journal: European Journal of Clinical Microbiology & Infect [Category] SARS, 임상,