A novel assessment method for COVID-19 humoral immunity duration using serial measurements in naturally infected and vaccinated subjectsArticle Published on 2022-09-292022-11-15 Journal: PLoS ONE [Category] SARS, 변종, 진단, [키워드] antibodies antibody Antigen applied Characteristics Collecting COVID-19 defined determine Dilution dilutions dose doses help humoral Humoral immunity immune response Infection information intensity linear long-lasting MONITOR Pfizer Prevent profile protective immunity quantification quantitative assay RBD reactivity Reinfection Sample SARS-CoV-2 SARS-CoV-2 antigens serological serology test serum sample subject two group vaccination Vaccination strategies Vaccine variant Viral antigen was used were used [DOI] 10.1371/journal.pone.0274553 PMC 바로가기
Lateral Flow Immunoassay Reader Technologies for Quantitative Point-of-Care TestingArticle Published on 2022-09-282022-11-15 Journal: Sensors (Basel, Switzerland) [Category] 치료제, [키워드] addition adopted Analysis anticipated Antigen assay quantification can be used carried caused colorimetric reader coronavirus disease COVID-19 Diagnosis dual-signal reader electrochemical reader Flow fluorescence reader function immunoassay introduced Lateral lateral flow lateral flow immunoassay Lateral flow immunoassays magnetic reader optical pandemic photothermal reader point-of-care point-of-care testing pregnancy test qualitative detection quantification Quantitative Quantitative analysis reduction Research technology Testing [DOI] 10.3390/s22197398 PMC 바로가기
Toward Next Generation Lateral Flow Assays: Integration of NanomaterialsArticle Published on 2022-09-282022-11-15 Journal: Chemical reviews [Category] Fulltext, MERS, 진단, [키워드] Bacterial complex COVID-19 diagnostic diagnostics feasibility Flow Generation highlight inherent integration Lateral limitation limits Nanomaterial pesticide platform point-of-care pregnancy test quantification receptor review sensitivity [DOI] 10.1021/acs.chemrev.1c01012 PMC 바로가기
Rational Design and Development of SARS-CoV-2 Serological Diagnostics by Immunoprecipitation-Targeted ProteomicsArticle Published on 2022-09-272022-11-15 Journal: Analytical Chemistry [Category] SARS, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] anti-RBD anti-SARS-CoV-2 antibody approach Combination convalescent convalescent plasma COVID-19 cross-reactivity current Cutoff detect development diagnostic Diseases domain elevated facilitate highest human immunoglobulin IgA IgA2 IGD IgE IgG IgG1 IgG2 IgG3 IgG4 IgM immune response immunoassay Immunoglobulin include isotype lack limit of detection limitation offer plasma Protein proteomic proteomics quantification Saliva SARS-CoV-2 serological Serological assay serological test serum significantly Simultaneous Standardization suggested [DOI] 10.1021/acs.analchem.2c01325 PMC 바로가기
Role of Demyelination in the Persistence of Neurological and Mental Impairments after COVID-19Article Published on 2022-09-252022-11-16 Journal: International Journal of Molecular Sciences [Category] 유전자 메커니즘, 치료기술, [키워드] acute phase Affect Brain changes in clinical evidence clinical study cognitive Cognitive impairment Combination Complication complications of COVID-19 COVID COVID-19 COVID-19 patients Demyelination detect disorders Factor fatigue Future help impairment include Inflammation Injury Insomnia knowledge lesions Long COVID manifestation mechanism Mental mental sequelae MRI Neuroimaging neurological neurological consequences Numerous Patient physiological post-COVID syndrome psychiatric disorder Psychological Quality of life quantification Quantitative Research role SARS-CoV-2 syndrome virus [DOI] 10.3390/ijms231911291 PMC 바로가기
Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future OutbreaksArticle Published on 2022-09-212022-11-15 Journal: Clinical microbiology reviews [Category] SARS, 치료기술, [키워드] acute respiratory syndrome Affect affecting application caused Clinical diagnosis coronavirus coronavirus disease Course COVID-19 ddPCR Detection limit Diagnosis diagnosis and monitoring Digital digital PCR effective Factor false-negative Future global public health gold standard help infected patient Infectious disease knowledge magnitude Mutation outbreak Outbreaks pandemic PCR Precision quantification reproducibility RT-dPCR RT-qPCR SARS-CoV-2 selected sensitivity shown specificity subject tested Viral load [DOI] 10.1128/cmr.00168-21 PMC 바로가기
Saliva is suitable for SARS-CoV-2 antibodies detection after vaccination: A rapid systematic reviewArticle Published on 2022-09-202022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, [키워드] 95% CI Ad26 analyzed anti-SARS-CoV-2 anti-SARS-CoV-2 vaccination antibodies antibody Antibody detection Antibody titer applied assays AstraZeneca convalescent individual correlation COVID-19 COVID-19 vaccines eligible ELISA assay evaluate flow cytometry highlight IgA IgG Immunity immunoassay Immunoglobulin immunological increasingly infected individuals janssen Johnson & Johnson lack moderate mRNA-based mucosal Neutralizing nine quantification reached reported Saliva salivary SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 detection selected serological test serum serum/plasma systematic review vaccinated individual vaccination Vaccination strategy Vaccine Web of Science [DOI] 10.3389/fimmu.2022.1006040 PMC 바로가기
Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization AssessmentArticle Published on 2022-09-202022-11-15 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 Analysis antibodies antibody approach assays assessment binding candidate Cell cellular cellular assay donors enzyme evaluate expression expression plasmid expression vector flow cytometric fluorescent fusion genomic information GFP insect insect cell insect cells labeling membrane membrane protein monoclonal antibody NTA Particle plasmid transfection Production purification quantification Rapid required SARS-CoV-2 SARS-CoV-2 envelope secondary antibody sera Spike protein TEM therapeutic transmission electron microscopy vaccinated individuals virus Virus-like particle Virus-like particles (VLPs) VLP VLPs [DOI] 10.3390/v14102087 PMC 바로가기
Circulating pyruvate is a potent prognostic marker for critical COVID-19 outcomesArticle Published on 2022-09-142022-11-15 Journal: Frontiers in Immunology [Category] SARS, 바이오마커, [키워드] abundance acute phase acute respiratory syndrome Alter Analysis approach assays body mass index C-reactive protein circulating clinical decision Compromised coronavirus COVID-19 COVID-19 pathogenesis COVID-19 patient creatinine criterion Critical Critically ill cut-off value determine disease disrupt driven by dysregulation energy-related metabolites evaluated Extracellular fluorometric quantification Glucose Health Host Host immune response immune response implicated IMPROVE Increased independent predictor Infection Lactate mechanisms metabolism metabolite metabolomics mitochondrial outcome outcomes Patient Potential biomarker Prognosis Prognostic marker provided pyruvate quantification Quantitative recruited risk SARS-CoV-2 SARS-COV-2 infection semi-targeted metebolomics. serum severe COVID-19 illness severity Signaling Stratification Support validation cohort virus [DOI] 10.3389/fimmu.2022.912579 PMC 바로가기
Development and Analytical Validation of a One-Step Five-Plex RT-ddPCR Assay for the Quantification of SARS-CoV-2 Transcripts in Clinical SamplesArticle Published on 2022-09-132022-11-16 Journal: Analytical Chemistry [Category] SARS, 치료기술, [키워드] analytical sensitivity Analytical specificity analyzed applied calibrator Characteristics clinical Concordance controls correlation COVID-19 pandemic development Direct DNA and RNA in-silico Inclusion Internal control linear methodology Metrics molecular primer probe provide Quality control quantification Region reproducibility RNA RT-qPCR Sample SARS-CoV-2 SARS-CoV-2 detection sensitivity Spearman specimen Standardization transcripts validation viral concentrations [DOI] 10.1021/acs.analchem.2c00868 PMC 바로가기