CRISPR/Cas12a-Based Diagnostic Platform Accurately Detects Nocardia farcinica Targeting a Novel Species-Specific GeneCRISPR/Cas12a 기반 진단 플랫폼, 새로운 종 특이적 유전자를 표적으로 하는 Nocardia farcinica를 정확하게 검출Article Published on 2022-05-272022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] 치료제, [키워드] accurate diagnosis applied Cas12a caused conserved COVID-19 pandemic CRISPR CRISPR-CLA cross-reaction detect detection system diagnose DNA DNA extraction Fluorescence fragment fragment of gold gold standard Infection Infectious disease isothermal amplification LAMP lateral flow limit of detection linker Loop-mediated isothermal amplification media meet Molecular diagnosis MONITOR Nocardia Nocardia farcinica nocardiosis novel nucleic acid Nucleic acid detection PAM pandemic pbr1. platform primer principle probe protospacer adjacent motif reaction real-time qPCR reference strain reported RNA screened sequence single-strand DNA specificity specimen sputum ssDNA strain target targeting tested Validity was used were used [DOI] 10.3389/fcimb.2022.884411 PMC 바로가기 [Article Type] Article
Digital screening for postnatal depression: mixed methods proof-of-concept studyArticle Published on 2022-05-232022-10-05 Journal: BMC Pregnancy and Childbirth [Category] 임상, [키워드] Analysis Antenatal appear assessment benefit Comparisons Complete consequence COVID-19 death Depression Digital digital mental health Edinburgh eligible enrolled evaluated examined feasibility feasible framework Framework analysis gestation Health Impact IMPROVE Infant lack lockdown Maternal Maternal mental health Mental mHealth Mixed methods Mobile Modification MONITOR MOST mother parent parental parents participant Postnatal depression pregnant women prevalent recruited Registered response Safe scale Screening secure Serious Adverse Event Smartphone study period suggested Treatment Validity women [DOI] 10.1186/s12884-022-04756-2 PMC 바로가기 [Article Type] Article
Mat-O-Covid: Validation of a SARS-CoV-2 Job Exposure Matrix (JEM) Using Data from a National Compensation System for Occupational COVID-19Article Published on 2022-05-082023-07-09 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), [키워드] Accuracy compensation COVID-19 JEM job exposure matrix occupational predictivity public health SARS-CoV-2 Validity work [DOI] 10.3390/ijerph19095733 PMC 바로가기
Adequacy of Serial Self-performed SARS-CoV-2 Rapid Antigen Detection Testing for Longitudinal Mass Screening in the Workplace직장에서 종방향 질량 스크리닝을 위한 연속 자가 수행 SARS-CoV-2 신속 항원 검출 테스트의 적절성Article Published on 2022-05-022022-09-11 Journal: JAMA Network Open [Category] COVID19(2023년), SARS, 치료기술, [키워드] Accuracy Adequacy age analyses analyzed antigen detection Asymptomatic Canada community transmission Contact contact with criteria Critical cross-sectional cross-sectional study detection feasibility Frequency improvement Improvements infected with SARS-CoV-2 Interpretation invalid longitudinal mass Measures median men Montreal Montreal, Canada not differ outcome participant Participants performed positive proportion proportions provided Public quarantine Rapid repeated required SARS-CoV-2 SARS-COV-2 infection Screening secondary Secondary outcomes significantly test validity Testing Validity was performed Workplace [DOI] 10.1001/jamanetworkopen.2022.10559 PMC 바로가기 [Article Type] Article
Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics디지털 PCR은 SARS-CoV-2 진단을 위한 RT-qPCR Cq 값의 해석을 보강할 수 있습니다.Article Published on 2022-05-012022-09-11 Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단, [키워드] acute health crisis acute respiratory disease Analysis bile salt bile salts caused common knowledge Compound coronavirus coronavirus disease Coronavirus disease 2019 coronavirus SARS-CoV-2 COVID-19 Cq value Cq value validity Cq values cut-off value cycle threshold demonstrated diagnostics Digital digital PCR disagreement disease dPCR Effect Efficiency effort exacerbated Health Health crisis highlight in urine include individual individuals infectious inhibiting inhibitor inhibitors Interpretation investigated knowledge MIQE and dMIQE guidelines molecular parameters PCR performed positive result qPCR quantification quantitative PCR Reporting Respiratory disease reverse transcription Reverse transcription quantitative PCR RNA extraction RT-qPCR Saliva Sample Sample matrix effects SARS-CoV-2 separated shown Standard Standard material susceptible Swab test results the disease These data Transmission Urine Validity Variability variable Viral viral burden Viral RNA Virus quantification [DOI] 10.1016/j.ymeth.2021.08.006 PMC 바로가기 [Article Type] Article
Feasibility and accuracy of the 40-steps desaturation test to determine outcomes in a cohort of patients presenting to hospital with and without COVID-19Observational Study Published on 2022-05-012022-10-05 Journal: Clinical medicine (London, England) [Category] COVID19(2023년), SARS, 임상, [키워드] 40-steps test Accuracy breathlessness change clinical utility cohort of patient COVID-19 death Desaturation Deterioration determine discharge Evidence exercise test. feasibility hospital less majority outcome participant pathology Patient patients with COVID-19 Predictive presenting prospective observational cohort readmission recruited respiratory rate saturation saturations screened suggested Validity [DOI] 10.7861/clinmed.2022-0027 PMC 바로가기 [Article Type] Observational Study
COVID-19: A literature review of the impact on diagnostic radiography studentsReview article Published on 2022-05-012022-10-05 Journal: Radiography (London, England : 1995) [Category] COVID19(2023년), SARS, 진단, [키워드] addition addressed Anxiety applied article assessment clinical Clinical placement contagious contracting the virus coronavirus COVID-19 diagnostic Effect eligibility criteria evaluate Evidence global pandemic Google Scholar hospital identify ill patients Impact literature review Mental mental health operators pandemic Personal protective equipment physically positive Radiographers Regular Result students Support supported Travel Validity viral disease [DOI] 10.1016/j.radi.2021.09.009 [Article Type] Review article
Qualitative analysis of hotspots and intrusive memories after viewing an aversive film highlights their sensory and spatial featuresRandomized Controlled Trial Published on 2022-04-292022-10-04 Journal: Scientific Reports [Category] 임상, [키워드] Analysis average coding COVID-19 feature Features FIVE highlight hotspot memory MOST participant Population qualitative analysis Randomized controlled trial Registered reported Result trauma Validity [DOI] 10.1038/s41598-022-10579-0 PMC 바로가기 [Article Type] Randomized Controlled Trial
Learning from COVID-19 related trial adaptations to inform efficient trial design-a sequential mixed methods study효율적인 시험 설계를 알리기 위해 COVID-19 관련 시험 적응에서 학습 - 순차적 혼합 방법 연구Review Published on 2022-04-292022-09-11 Journal: BMC Medical Research Methodology [Category] 임상, [키워드] adaptation Administered barrier benefit clinical clinical trial clinical trials concerning Consent COVID-19 COVID-19 pandemic Efficiency efficient Efficient trial design eligibility assessment Evidence flexibility Follow-up identify IMPROVE include Intervention delivery investigated lack learning limitation literature review methodology NHS not seen Other pandemic participant Patient recruitment Registered selected Support supported SWAT tested Trial Trial methodology Trial methodology. trials unit Validity workshop [DOI] 10.1186/s12874-022-01609-6 PMC 바로가기 [Article Type] Review
Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysisSYBR Green Melting Curve 분석을 이용한 SARS-CoV-2 감염 검출을 위한 비용 효율적인 1단계 다중 RT-PCR 분석법 개발 및 검증Article Published on 2022-04-202022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, 치료제, [키워드] combinations COVID-19 diagnosis detect development Diagnostic method E gene extracted RNA Host house Inc. Infection initial initial stage Internal control melting curve analysis multiplex Multiplex assay optimization PCR PCR reaction primer provided purification randomly selected sample ranged RdRP RdRp gene RdRp genes RNA extraction RT-PCR RT-PCR assay SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 virus sensitivity specificity subset SYBR Green TaqMan TaqMan probe the SARS-CoV-2 the SARS-CoV-2 virus Validity Variation Viral RNA were used β-actin [DOI] 10.1038/s41598-022-10413-7 PMC 바로가기 [Article Type] Article