Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis
Research
[키워드] amplified
analysed
antibody
Antigen
association
bronchitis
cDNA
Cell
Chromatography
coding region
coding sequence
Combined
combined use
competent
Concentration
confidence interval
controls
demonstrated
detect
develop
Diagnosis
E. coli
early diagnosis
ELISA
Escherichia coli
evaluate
examined
expected
expressed
expression vector
GE Healthcare
GraphPad Prism
GraphPad software
greater
IBV
indirect ELISA
Infectious Bronchitis Virus
Invitrogen
liquid
molecular
N protein
nucleocapsid protein
nucleoprotein
PCR
positive
powerful tool
Prevent
primer
Protein
purification
purified
receiver-operating characteristics curves
recombinant antigen
required
Result
RT-PCR
scored
SDS-PAGE
sensitivity
sera
serum dilution
serum sample
specificity
subunit
Test
tested
the disease
the N protein
USA
was obtained
were used
Western blot analysis
window
yielding
[DOI] 10.1186/s12985-018-1096-2 PMC 바로가기 [Article Type] Research
[DOI] 10.1186/s12985-018-1096-2 PMC 바로가기 [Article Type] Research