Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platformArticle Published on 2020-11-302022-10-29 Journal: Nature Communications [Category] COVID-19, SARS, [키워드] Antigen Cancer caused cellular immune responses Coronavirus-2 develop development DNA expressing generate global pandemic humoral immunized immunodominant implicated Infectious disease Live attenuated vaccines mice Neutralizing antibodies nucleocapsid antigen nucleocapsid antigens platform poxvirus protective immunity robust SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 vaccine stimulate unique Vaccine vaccine candidate vaccine platform vaccinia vector viral infection [DOI] 10.1038/s41467-020-19819-1 PMC 바로가기 [Article Type] Article
Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2SARS-CoV-2에 대해 설계된 단백질 나노입자 백신에 의한 강력한 중화 항체 반응의 유도Article Published on 2020-11-252022-09-12 Journal: Cell [Category] Fulltext, SARS, 비임상, 진단, [키워드] antibody Antibody Response Antigen cGMP clinical trial computational protein design convalescent displaying domain ectodomain effective effort elicit elicited Epitopes highlight human sera immunogen immunogenic induce lower dose mice mutations nanoparticle Neutralizing antibody response Neutralizing antibody titer non-overlapping epitope platform potent Protective protective antibody response Protein RBD RBDs Receptor-binding domain Respiratory disease response risk robust Safe SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 spike SARS-CoV-2 vaccine SARS-CoV-2-RBD stability structure-based design susceptible the RBD the SARS-CoV-2 trimer utility Vaccine vaccine candidate vaccine for COVID-19 virus wall [DOI] 10.1016/j.cell.2020.10.043 PMC 바로가기 [Article Type] Article
An in silico LLPS perturbation approach in the design of a novel SARS-CoV-2 spike receptor-binding domain inhibitorCorrespondence Published on 2020-11-252022-11-01 Journal: The Egyptian Journal of Medical Human Genetics [Category] COVID-19, [키워드] approach biological processe biological systems chromatin domain homogenous in silico include inhibitor membrane envelope metabolism Nucleolus organelle Regulation resulting RNA SARS-CoV-2 spike shown Signal transduction stress granules [DOI] 10.1186/s43042-020-00105-0 PMC 바로가기 [Article Type] Correspondence
Massive dissemination of a SARS-CoV-2 Spike Y839 variant in Portugal포르투갈에서 SARS-CoV-2 Spike Y839 변종의 대규모 보급Research Article Published on 2020-11-252022-09-10 Journal: Emerging Microbes & Infections [Category] MERS, SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] anti-viral drug Anti-viral drugs collaboration confirmed case contributing to country COVID-19 COVID-19 case D839Y dissemination Effect Epidemic Epidemics experimental validation exponential phase Frequency functional fusion peptide Genetic variant Genome genomes Genomic epidemiology Genomic surveillance Italy massive motif Mutation peptide Portugal prevalent Region relative frequency required SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant selective Sequencing spike spike variants Spread Support Surveillance the epidemic the Spike variant variants [DOI] 10.1080/22221751.2020.1844552 PMC 바로가기 [Article Type] Research Article
Rapid identification of a human antibody with high prophylactic and therapeutic efficacy in three animal models of SARS-CoV-2 infectionSARS-CoV-2 감염의 3가지 동물 모델에서 높은 예방 및 치료 효능을 가진 인간 항체의 신속한 식별530 Published on 2020-11-242022-09-12 Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 진단, [키워드] Ab1 ACE2 ADCC ADCC activity Administered aggregate aggregation animal model animal models antibody antibody-dependent cellular cytotoxicity Antigen assays BALB/c mice binding Combination competition Complete Coronaviruses COVID-19 Culture dose effective Efficacy elicited exhibited expressing Fab glycoprotein hACE2 hamster hamsters human ACE2 human cell human cell membrane human monoclonal antibodies Human monoclonal antibody IgG1 in vitro inhibited lack lowest mAb mAbs mechanism microbes monoclonal neutralization neutralized pandemic Prophylactic prophylactically Prophylaxis Protein Proteins public health threat Rapid RBD Receptor binding domain replication-competent SARS-CoV-2 SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 replication SARS-CoV-2 spike scFv sequence Significance somatic mutations tested the SARS-CoV-2 therapeutic antibodies therapeutic efficacy therapy tissue tissue culture transgenic mice Vaccines virus virus infection was selected wild-type [DOI] 10.1073/pnas.2010197117 PMC 바로가기 [Article Type] 530
Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment내열성이 높은 면역원성 SARS-CoV-2 스파이크 단편의 설계Research Article Published on 2020-11-232022-09-12 Journal: The Journal of Biological Chemistry [Category] SARS, 진단, [키워드] ACE2 antibodies AUC, area under the curve clinic clinical testing COVID-19 CPE, cytopathic effect Culture DMEM, Dulbecco's Modified Dulbecco's Medium ectodomain elicited Endpoint exposure to expressed extreme feature geometric mean geometric mean titer glycosylation guinea pig HRP, horseradish peroxidase IAEC, Institutional Animal Ethics committee IMAC, immobilized metal affinity chromatography immunogenic ingredient mammalian cell MF59 microbial monomeric mRNA Neutralizing antibodies neutralizing antibody NTD, N-terminal domain PBS, phosphate buffered saline PEI, polyethylenimine Pichia Protein subunit vaccines purified RBD RBD protein RBD, receptor-binding domain RBM, receptor binding motif Receptor-binding domain replicative SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 vaccine SEC, size-exclusion chromatography solution Spike protein subunit Subunit vaccine temperature the RBD the receptor-binding domain thermostable thermotolerance Vaccine viral spike protein Viral vector viral vectors virus [DOI] 10.1074/jbc.RA120.016284 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected hospitalized COVID-19 patientsSARS-CoV-2 성장, 퓨린 절단 부위 적응 및 급성 감염 입원 COVID-19 환자의 혈청을 사용한 중화Research Article Published on 2020-11-212022-09-10 Journal: The Journal of General Virology [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus adaptation angiotensin antigenic target Betacoronavirus both viruses causative agent caused Cell cell lysate cell surface cells characterized cleavage clinical isolates comparable coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 deaths deleted Deletion demonstrated disease expand furin furin cleavage generate glycoprotein growth hACE2 Healthcare system Healthcare systems hospitalized COVID-19 patient host range human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 in vitro in vivo indirect immunofluorescence inefficient Infection insertion investigated Like MinION Munich neutralization Neutralizing antibodies neutralizing antibody titre neutralizing antibody titres Pathogenesis patients reached receptor respiratory S glycoprotein S protein SARS-CoV SARS-CoV-2 SARS-CoV-2 spike Sequencing serum serum sample severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spread to strain the cell the S protein the SARS-CoV-2 variant Vero E6 Vero E6 cell VERO E6 cells virus virus stock virus stocks viruses [DOI] 10.1099/jgv.0.001481 PMC 바로가기 [Article Type] Research Article
Neutralizing antibody against SARS-CoV-2 spike in COVID-19 patients, health care workers, and convalescent plasma donorsCOVID-19 환자, 의료 종사자 및 회복기 혈장 기증자의 SARS-CoV-2 급증에 대한 중화 항체Technical Advance Published on 2020-11-192022-09-12 Journal: JCI Insight [Category] SARS, 진단, [키워드] antibody Antibody testing Cellular immune response cohorts convalescent plasma COVID-19 COVID-19 pathogenesis COVID-19 patients COVID-19 symptoms Donor donors Efficacy evaluate followed by glycoprotein HCWs Health care Health care worker health care workers highest highlight HIV-1 hospitalized patients human antibody response ICU ICU Patients individual individuals intensive care intensive care unit intron lentiviral lentivirus Neutralization assay neutralizing antibody Neutralizing antibody titer neutralizing antibody titers Patient phenotypic phenotypic variation pseudotype pseudotyped Rapid reporter SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 spike SARS-CoV-2 strain secreted serological survey Serological surveys serum the SARS-CoV-2 Treatment Variation Virus neutralization was used [DOI] 10.1172/jci.insight.143213 PMC 바로가기 [Article Type] Technical Advance
Target-Centered Drug Repurposing Predictions of Human Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Protease Serine Subtype 2 (TMPRSS2) Interacting Approved Drugs for Coronavirus Disease 2019 (COVID-19) Treatment through a Drug-Target Interaction Deep Learning Model인간 안지오텐신 전환 효소 2(ACE2) 및 막관통 프로테아제 세린 아형 2(TMPRSS2) 상호 작용에 대한 표적 중심 약물 용도 변경 예측Article Published on 2020-11-182022-08-31 Journal: Viruses [Category] SARS, 신약개발, [키워드] ACE ACE2 ACE2 receptor acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affinity angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 approval approved drug AT1R candidate clinically Control coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 daclatasvir deep deep learning drug Drug repurposing drug-target drugs enalaprilat eprosartan FDA FDA approved drug food gene expression profile HCV Host host cell Human inhibit inhibitor inhibitory potency Interaction learning less lisuride Model molecule Neuropsychiatric ombitasvir overcome overlapped paritaprevir portal predict predicted prediction protease receptor Remdesivir Replication reported repurposing respiratory SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 spike Serine serine 2 severe acute respiratory syndrome Coronavirus subtype target TMPRSS2 TMPRSS2 inhibitors transmembrane transmembrane protease serine 2 Treatment Viral viral entry virus [DOI] 10.3390/v12111325 PMC 바로가기 [Article Type] Article
Spike-specific circulating T follicular helper cell and cross-neutralizing antibody responses in COVID-19-convalescent individuals Research Published on 2020-11-162022-10-05 Journal: Nature Microbiology [Category] SARS, 치료기술, 치료법, [키워드] acute respiratory syndrome antibody Antibody Response Asymptomatic autoantibodies B-cell benefit caused Cell circulating coronavirus Coronavirus disease 2019 COVID-19 COVID-19-convalescent individual COVID-19-convalescent individuals cross-neutralizing antibody response cross-reactivity detectable disease early phase facilitate faster follicular Frequency functional help increase in individual individuals with COVID-19 Infection Lymphocyte count majority Middle East Mild moderate Neutralizing activity neutralizing antibody Neutralizing antibody response neutralizing antibody titre neutralizing antibody titres other coronavirus Patient Pseudotyped virus Pseudotyped viruses respiratory syndrome coronavirus SARS-CoV SARS-COV-2 infection SARS-CoV-2 spike serum severe COVID-19 Symptom symptom onset the antibody the SARS-CoV-2 Treatment Vaccine Vaccine development [DOI] 10.1038/s41564-020-00824-5 [Article Type] Research