Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patientsResearch Articles Published on 2020-10-082022-10-28 Journal: Science Immunology [Category] COVID-19, MERS, SARS, [키워드] Analysis anti-SARS-CoV-2 antibody Antibody Response biofluids Blood convalescent patient correlation COVID-19 COVID-19 patient domain ELISAs enzyme-linked immunosorbent assay full length IgA IgA response IgG IgG antibody IgG level IgG response IgM IgM antibody IgM response Immunity longitudinal majority negative controls positively correlated post-symptom onset PSO RBD RBD antigen remained responses Saliva SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike antigen serum serum IgG Spike protein the antibody response the SARS-CoV-2 trimer while [DOI] 10.1126/sciimmunol.abe5511 PMC 바로가기 [Article Type] Research Articles
Highly conserved binding region of ACE2 as a receptor for SARS-CoV-2 between humans and mammalsBrief Report Published on 2020-09-292022-10-28 Journal: The Veterinary Quarterly [Category] COVID-19, MERS, SARS, [키워드] accelerate ACE2 ACE2 protein acute respiratory syndrome addition Amino acid Analysis angiotensin-converting enzyme 2 antiviral therapeutics appear binding binding region Complete conserved coronavirus COVID-19 FIVE food homologous homology Human human ACE2 humans infected with SARS-CoV-2 Infection information Interaction involved mammal Medical countermeasures mink Multiple sequence alignment Nature New York peptide Phylogenetic tree Precision Protein protein regions Quality RBD receptor Receptor binding domain reduced reported residue risk SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD SARS-CoV-2 transmission sequence spike glycoprotein structural homology Support the SARS-CoV-2 three-dimensional structure transmitted USA Vaccine design virus was used were infected World Health Organization [DOI] 10.1080/01652176.2020.1823522 PMC 바로가기 [Article Type] Brief Report
SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primatesResearch Article Published on 2020-09-242022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome aluminium adjuvant Analysis antigenicity appear candidate convalescent coronavirus coronavirus disease COVID-19 COVID-19 patient dose ectodomain elicit ELISA enzyme-linked immunosorbent evaluated expressed expression glycosylation highly pathogenic homotrimer immunization immunogenicity Inclusion induce insect cell insect cell expression system lowest mammalian cell mice Microscopy monomer neutralization non-human primate offer pandemic prefusion produced Protein RBD reactivity Receptor binding domain recombinant COVID-19 vaccine reduced regimen S protein S-2P S-protein S1 subunit SARS-CoV-2 SARS-CoV-2 spike sera spike subjects suggested the mean therapeutic titre Transmission trimer Vaccine wild-type [DOI] 10.1080/22221751.2020.1821583 PMC 바로가기 [Article Type] Research Article
Molecular docking between human TMPRSS2 and SARS-CoV-2 spike protein: conformation and intermolecular interactionsResearch Article Published on 2020-09-242022-10-27 Journal: AIMS Microbiology [Category] COVID-19, [키워드] ACE2 attribute C-terminal cleavage Characteristics cleavage cleavage site cleavage sites complex conformational COVID-19 directional docked docking driven by drug target etiological agent full length functional host cell human ACE2 receptor interact Interaction intermolecular interaction molecular molecular docking N-terminal predicted priming provide residue resolved responsible SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein serine protease Spike protein the SARS-CoV-2 the spike protein TMPRSS2 viral spike protein [DOI] 10.3934/microbiol.2020021 PMC 바로가기 [Article Type] Research Article
Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients스파이크, 막 및 뉴클레오캡시드 SARS-CoV-2 단백질에 대한 강력한 T 세포 반응은 심각한 COVID-19 환자의 회복과 관련이 없습니다Article Published on 2020-09-222022-09-11 Journal: Cell Reports Medicine [Category] MERS, SARS, 진단, 치료법, [키워드] Activation CD4 CD8 clearance comparable COVID-19 COVID-19 severity Critical critical COVID-19 critical COVID-19 patient critical COVID-19 patients critical patients Diagnosis dominance donors effector T cell Frequency highest Hyperreactivity Hypothesis Immunity Immunopathogenesis immunopathology indicate induce magnitude membrane memory moderate N protein nucleocapsid patients peptide peptide pool Protein Proteins response robust S/M/N protein-reactive T cells SARS-CoV-2 SARS-CoV-2 spike spike Support survival T cell T cell response T cell responses T cells vaccination virus clearance [DOI] 10.1016/j.xcrm.2020.100092 PMC 바로가기 [Article Type] Article
Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunityResearch Article Published on 2020-09-172022-10-30 Journal: JCI Insight [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Adaptive immunity antibody Antibody detection Antibody Response binding antibody circulating antibody collected coronavirus correlation COVID-19 COVID-19 immunity detection ELISA exhibited Generation Health healthy IgG antibody immune response Immunity immunology implication individual moderate N-protein neutralize Neutralizing antibodies neutralizing capacity nucleocapsid pandemic patients positive Protein Quantitative Receptor binding domain reverse transcription S-RBD SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 spike sera Seroconversion serological serology assay serology testing serum sample Spike protein the SARS-CoV-2 Viral protein virus infection [DOI] 10.1172/jci.insight.142386 PMC 바로가기 [Article Type] Research Article
Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domainArticle Published on 2020-09-102022-10-04 Journal: Nature Communications [Category] 변종, 치료제, [키워드] ACE2 acute respiratory syndrome antibody Antibody fragment therapy antiviral therapies association binding kinetics blockade Concentration coronavirus domain entry receptor experiment fusion generate Host human IgG1 Fc humanized identify IMPROVE in vitro inhibit inhibiting Interaction monomeric neutralization neutralization activity neutralize Neutralizing Proteins RBD receptor ACE2 Receptor binding domain SARS- CoV-2 SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 spike significantly Spread Support Therapies viral entry viral infection [DOI] 10.1038/s41467-020-18387-8 PMC 바로가기 [Article Type] Article
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interactionArticle Published on 2020-09-042022-10-04 Journal: Nature Communications [Category] SARS, 진단, [키워드] ACE2 antibodies antibody Bacteria bind binding complex conformation conformations COVID-19 Cryo-electron microscopy Cryoelectron microscopy domain enzyme epitope expressed high affinity host cell identify immunize Interaction Intervention Isolation nanobody neutralize presenting pseudovirus RBD RBD-ACE2 receptor Receptor binding domain reveal SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 spike SARS-CoV-2 spike protein spike glycoprotein target the RBD the SARS-CoV-2 while [DOI] 10.1038/s41467-020-18174-5 PMC 바로가기 [Article Type] Article
Naturally occurring SARS-CoV-2 gene deletions close to the spike S1/S2 cleavage site in the viral quasispecies of COVID19 patientsResearch Article Published on 2020-09-022022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] accumulate ACE2 receptor binding Burn circulation Complete COVID19 patient Deletion deletions described Diversity facilitate frameshift generate Genome host cell Infection insertions Mild Mutation rates natural selection Naturally Neutralizing antibodies NGS other coronaviruses Patient Protein Quasispecies Recombination reducing released respiratory virus S1 protein S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 S gene SARS-CoV-2 spike severe COVID-19 severe COVID-19 patients severity stop codon tissue damage Transmission Treatment upstream Vaccine development viral particle viral quasispecy virus [DOI] 10.1080/22221751.2020.1806735 PMC 바로가기 [Article Type] Research Article
Nano-clays as Potential Pseudo-antibodies for COVID-19Nano Idea Published on 2020-08-282022-10-30 Journal: Nanoscale Research Letters [Category] COVID-19, [키워드] Cancer carrier coronavirus COVID-19 effective hACE2 hACE2 protein high affinity humans in vitro in vivo injected Interaction Melanoma Metastasis performed Potential provided Research SARS-CoV-2 spike Treatment vaccine for COVID-19 virus [DOI] 10.1186/s11671-020-03403-z PMC 바로가기 [Article Type] Nano Idea