The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirusResearch Article Published on 2008-11-282022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] absence analyzed antibody Antigen antigenicity antiviral efficacy assays BALB/c mice cloned coronavirus cross-reactivity determined diagnostic effective drugs effective vaccine ELISA Escherichia coli evaluate expressed expression expression vector fluorescent fusion protein HCoV 229E human coronavirus human coronaviruses humoral IFA immunization immunized mice immunoblot induce inhibit injected injection intranasally Laboratory test management mice National neutralization nucleocapsid OC43 Protein Quantitative reactivity Result RT-PCR SARS patient SARS virus Sensitivity and specificity sera T cell proliferation T-cell analysis T-cell Response vaccine candidate Viral load virus replication was performed [DOI] 10.1186/1471-2180-8-207 PMC 바로가기 [Article Type] Research Article
Dynamic changes of serum SARS-Coronavirus IgG, pulmonary function and radiography in patients recovering from SARS after hospital dischargeResearch Published on 2005-01-082022-10-28 Journal: Respiratory Research [Category] Coronavirus, [키워드] acute respiratory syndrome addition Avascular necrosis of femoral head Beijing change changes changes in chest X-ray China Chinese clinically Computerized tomography detected diagnosed diffusion discharge dynamic evaluate examined exhibited fibrosis finding Follow-up found hospital hospital discharge Hospitalized HRCT IgG IgG antibody incidence included individual joint lack lung Lung fibrosis Lung function magnetic resonance imaging MRI Necrosis objective Pain Patient patients positive predicted pulmonary fibrosis Pulmonary function receiving SARS Coronavirus SARS patient SARS-CoV SARS-CoV IgG antibody serum Serum level Severe acute respiratory syndrome (SARS) significantly subject subjects tested transfer were given [DOI] 10.1186/1465-9921-6-5 PMC 바로가기 [Article Type] Research
Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periodsArticle Published on 2004-12-012022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] assays average clinical clinically suspected collected Course CoV detected Diagnosis disease Epidemic evaluate exhibited facilitated Germany highlight Hong Kong Laboratory lack nucleic acid extraction outbreak PCR assay PCR assays Positive predictive value protocol Rapid diagnosis Real-time PCR real-time PCR assay respiratory specimen reverse transcription RNA extraction Roche Roche Diagnostics RT-PCR SARS Coronavirus SARS CoV SARS epidemic SARS patient sensitivity serum sample shown specificity specimen specimens Stool tested the SARS epidemic Urine WHO [DOI] 10.1016/j.jcv.2004.09.029 PMC 바로가기 [Article Type] Article
Early diagnosis of SARS Coronavirus infection by real time RT-PCRArticle Published on 2003-09-242022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] 2003 assay collected develop Diagnosis diagnostic disease onset early early diagnosis early stage evaluate Infection Lancet Modification nasopharyngeal negative control sample negative controls NO Novel coronavirus positive protocol Quantitative quantitative PCR real time real time RT-PCR real-time RT-PCR respiratory Result RNA extraction RT-PCR RT-PCR assay Sample SARS Coronavirus SARS CoV SARS patient SARS-coronavirus sensitivity Serologically severe specimen tested viral RNA extraction [DOI] 10.1016/j.jcv.2003.08.004 PMC 바로가기 [Article Type] Article