Evaluation of High-Throughput SARS-CoV-2 Serological Assays in a Longitudinal Cohort of Patients with Mild COVID-19: Clinical Sensitivity, Specificity, and Association with Virus Neutralization Test경증 COVID-19 환자의 세로 코호트에서 처리량 SARS-CoV-2 혈청학적 분석 평가: 임상 민감도, 특이도 및 바이러스 중화 테스트와의 연관성Article Published on 2021-04-292022-09-11 Journal: Clinical Chemistry [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] Antibody Response Antibody titers Area association AUC best can not clinical clinical sensitivity clinical specificity collected Concordance Course COVID-19 detect DiaSorin disease evaluated except for functional greater health-care Health-care workers healthcare worker IgA IgG IgM longitudinal lowest mild patient mild patients neutralization test Neutralizing antibodies neutralizing antibody neutralizing antibody titers Neutralizing titer ranged RBD Receptor binding domain ROC Curve RT-PCR S protein SARS-CoV-2 sensitivity sera serological Serological assay serological assays Serological tests serum Siemens specificity specimen subset Test the RBD the S protein Total Virus neutralization virus neutralization test VNT with COVID-19 [DOI] 10.1093/clinchem/hvaa336 PMC 바로가기 [Article Type] Article
Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced seraArticle Published on 2021-04-292022-10-31 Journal: Cell [Category] COVID-19, MERS, SARS, [키워드] abrogate Abstract ACE2 ACE2 binding acute respiratory syndrome Analysis angiotensin-converting enzyme-2 antibody AstraZeneca B.1.351 binding Brazil changes in convalescent coronavirus domain driven by E484K escape evidence of facilitate form Immune escape immunodominant K417N large cohort Lineage mAb monoclonal antibody Mutation N501Y neutralization neutralization titer Neutralizing NTD offer P.1 Pfizer receptor receptor ACE2 Receptor-binding domain reduced reveal SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 variant sequence sera serum serum sample serum samples South Africa Spike protein structure-function the SARS-CoV-2 vaccinated individual Vaccine vaccinee variant viral cell viral spike protein widespread [DOI] 10.1016/j.cell.2021.02.037 PMC 바로가기 [Article Type] Article
SARS-CoV-2 501Y.V2 variants lack higher infectivity but do have immune escapeSARS-CoV-2 501Y.V2 변이체는 더 높은 감염성은 없지만 면역 탈출은 있습니다Article Published on 2021-04-292022-09-11 Journal: Cell [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] 501Y.V2 Abstract caused Cell cell type cell types cells convalescent convalescent patient Convalescent patients dominant E484K Efficacy escaped except for exhibit experiment hACE2 higher infectivity Immune escape immunized immunized mice increase in individuals Infectivity inhibitory effect Inhibitory effects K417N lack mACE2 mice monoclonal antibodies monoclonal antibody multiple mutation multiple mutations murine Mutation N501Y N501Y mutation neutralization neutralization ability neutralizing antibody neutralizing monoclonal antibodies neutralizing monoclonal antibody polyclonal antibodies polyclonal antibody Pseudotyped virus Pseudotyped viruses receptor binding region Receptor-binding domain reduced SARS-CoV-2 sera significantly South Africa Spike protein susceptibility the receptor-binding domain Vaccines variant variant of SARS-CoV-2 variants variants of SARS-CoV-2 Viral viral spike protein viruses [DOI] 10.1016/j.cell.2021.02.042 PMC 바로가기 [Article Type] Article
Performance of an automated chemiluminescent immunoassay for SARS-COV-2 IgM and head-to-head comparison of Abbott and Roche COVID-19 antibody assaysFull Length Article Published on 2021-04-282022-10-29 Journal: Practical Laboratory Medicine [Category] COVID-19, [키워드] 95% CI Abbott analysed antibodies antibody antibody assay Architect Assay evaluation automated chemiluminescent immunoassay Cohen’s kappa comparable Control COVID-19 COVID-19 antibody cross-reactivity days post infection develop disease disorder dsDNA evaluated Head-to-head comparison healthy control healthy controls IgM IgM and IgG IgM/IgG immunoassay IMPROVE individual moderate outperform performance POS positive positive RT-PCR recruited Result Roche RT-PCR SARS-CoV-2 SARS-CoV-2 IgM SARS-CoV-2 RT-PCR sensitivity sera significantly specificity total antibody were assessed while [DOI] 10.1016/j.plabm.2021.e00230 PMC 바로가기 [Article Type] Full Length Article
Temporal Dominance of B.1.1.7 over B.1.354 SARS-CoV-2 Variant: A Hypothesis Based on Areas of Variant Co-CirculationArticle Published on 2021-04-222022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] Analysis Area B.1.1.7 B.1.351 biological properties co-circulation community transmission convalescent dominance Effectiveness examined GISAID database Heterologous Hypothesis introduced mAbs Mayotte monoclonal antibody pre-existing immunity proportion raise reported SARS-CoV-2 SARS-CoV-2 sequence SARS-CoV-2 variant sera South Africa Strains Temporal Transmissibility transmitted vaccination Vaccine Vaccines variant variants variants of concern VoC [DOI] 10.3390/life11050375 PMC 바로가기 [Article Type] Article
Humoral immune responses in hospitalized COVID-19 patientsOriginal Article Published on 2021-04-202022-10-04 Journal: Saudi Journal of Biological Sciences [Category] 유전자 메커니즘, [키워드] acute respiratory syndrome Analysis anti-S IgG Anti-SARS-CoV-2 S antibodies antibody antibody isotype association caused coronavirus Coronavirus 2019 COVID-19 COVID-19 patient detect Diagnosis diagnosis of COVID-19 disease disease severity ELISA enzyme-linked immunosorbent female females Hospitalized hospitalized COVID-19 patient hospitalized COVID-19 patients hospitalized patient humoral immune response Humoral immunity IgA IgA antibodies IgA antibody IgA response IgG IgG and IgM IgM IgM antibody immune response Immunity Infection investigated isotype male material Non-severe non-severe patients participated Patient patients positive Protein public health crisis Qualitative response Result RT-PCR SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 RNA Saudi Arabia sera severe significantly significantly higher Specific the S protein the SARS-CoV-2 Variation Viral proteins virus was used were measured [DOI] 10.1016/j.sjbs.2021.04.032 PMC 바로가기 [Article Type] Original Article
SARS-CoV-2 Antibody Screening in Healthcare Workers in Non-Infectious Hospitals in Two Different Regions of Southern Poland (Upper Silesia and Opole Voivodeships): A Prospective Cohort Study폴란드 남부의 서로 다른 두 지역(Upper Silesia 및 Opole Voivodeships)의 비감염성 병원 의료 종사자를 대상으로 한 SARS-CoV-2 항체 검사: 전향적 코호트 연구Article Published on 2021-04-202022-09-10 Journal: International Journal of Environmental Research an [Category] MERS, SARS, 치료기술, [키워드] antibody screening Asymptomatic benefit blood collection coronavirus coronavirus SARS-CoV-2 COVID-19 detection determine different time point ELISA test emergency wards evaluated had no HCW HCWs health-care professionals healthcare healthcare worker Healthcare workers hospital Hospital environment Host human coronavirus IgA IgG IgM IgM/IgG immunoglobulins individual Infection Local negative RT-PCR New coronavirus non-infectious patients PCR test PCR testing performed Poland Population positive Prevalence Prospective reduce Region risk RT-PCR RT-PCR test RT-PCR testing sampling SARS-CoV-2 SARS-COV-2 infection Screening semi-quantitative ELISA sera serologic responses serological surveillance Seropositivity socio-demographic Spread Symptoms symptoms of infection test results university university hospital upper Volunteer worker [DOI] 10.3390/ijerph18084376 PMC 바로가기 [Article Type] Article
Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera회복기 및 백신 혈청에 의한 SARS-CoV-2 B.1.1.7 변이체의 중화 감소Article Published on 2021-04-152022-09-11 Journal: Cell [Category] MERS, SARS, 변종, 진단, [키워드] Abstract ACE2 ACE2 binding Amino acid amino acid change Analysis antibody Antibody Response Antibody responses appearance B.1.1.7 B.1.1.7 variant binding affinity can not caused Contact convalescent death dominant elicited ENhance escape evade generate IGHV3-53 Kent monoclonal antibodies monoclonal antibody mutant virus mutant viruses N501Y natural antibody natural infection neutralization neutralize neutralized original original form pandemic parental reduced residue responses responses against SARS-CoV-2 SARS-CoV-2 B.1.1.7 SARS-COV-2 infection sera Transmission vaccination Vaccine Vaccines variant virus virus replication Virus spike viruses widespread [DOI] 10.1016/j.cell.2021.02.033 PMC 바로가기 [Article Type] Article
SARS-CoV-2 IgG Antibodies Seroprevalence and Sera Neutralizing Activity in MEXICO: A National Cross-Sectional Study during 2020Article Published on 2021-04-152022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] activity Acute respiratory infection analyzed Antibody neutralization Blood Bank carried CLIA clinical laboratory country COVID-19 cumulative determine diagnostic methods ELISA enzyme-linked immunosorbent epidemiological Fever General population herd immunity IgG Immunity immunosorbent incidence incidence of COVID-19 Infection molecular National neutralization neutralizing antibody Patient positive reported S1/S2 sampling SARS-CoV-2 IgG SARS-COV-2 infection security sera SEROLOGICAL STUDY serology Seroprevalence specimen study period the SARS-CoV-2 Transmission dynamics [DOI] 10.3390/microorganisms9040850 PMC 바로가기 [Article Type] Article
Infection- and vaccine-induced antibody binding and neutralization of the B.1.351 SARS-CoV-2 variantB.1.351 SARS-CoV-2 변이체의 감염 및 백신 유도 항체 결합 및 중화Article Published on 2021-04-142022-09-11 Journal: Cell Host & Microbe [Category] MERS, SARS, 변종, 진단, [키워드] additional spike mutation additional Spike mutations Antibody binding Antibody titer Antibody titers B.1 B.1.351 B.1.351 variant binding antibody convalescent COVID-19 patient D614G deletions domain Efficacy emerging variants exhibited humoral Humoral immunity individual individuals Infection Live virus Moderna Mutation neutralization neutralize Neutralizing antibody titer neutralizing antibody titers polyclonal antibodies polyclonal antibody protective humoral immunity raising Receptor-binding domain reduced reduction in retained SARS-CoV-2 SARS-CoV-2 B.1.351 variant SARS-CoV-2 variant SARS-CoV-2 variants sera spike mutation Spike protein the spike protein vaccinated individual Vaccine vaccine-induced antibodies variant Viral viral neutralization [DOI] 10.1016/j.chom.2021.03.009 PMC 바로가기 [Article Type] Article