Identification of Distinct Immune Cell Subsets Associated With Asymptomatic Infection, Disease Severity, and Viral Persistence in COVID-19 PatientsImmunology Published on 2022-02-222022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] Analysis Apoptosis asymptomatic infection asymptomatic subject CD14 CD160 CD33 CD68 CD8 Cell CLEC10A clinical presentation comprehensive analysis COVID-19 CSF1R develop Differentially expressed genes disease severity ENhance enriched pathway help HLA-DMA identification IL1B immune response immune responses Immunopathogenesis Inflammation KEGG LAG3 monocyte NCAM1 pathogenic PBMC positively correlated Protective S100A9 SARS-CoV-2 SARS-COV-2 infection scRNA-seq severity significantly singlecell RNA sequencing (scRNA-seq) subset surface marker Symptomatic patients the disease TRAV1-2 unique viral persistence [DOI] 10.3389/fimmu.2022.812514 PMC 바로가기 [Article Type] Immunology
Integrating single-cell sequencing data with GWAS summary statistics reveals CD16+monocytes and memory CD8+T cells involved in severe COVID-19Research Published on 2022-02-172022-10-31 Journal: Genome Medicine [Category] COVID-19, MERS, [키워드] ABO airways alteration association calculate CCR1 CD8+ T cell CD8+T cell Cell cell types Cell-mediated immunity cells chemotaxis collected contribute Control COVID-19 COVID-19 infection COVID-19 pandemic CXCR6 cytokine detrimental disease severity dysregulated immune response Effect effective vaccine elevated epithelial cell expressed expressed gene expression facilitate Factor feature functional Genes Genetic genetic architecture genetic determinant GWAS GWAS summary statistics healthy control higher risk Host host genetics IFITM1 Immune cell immune cells immune response Immunity immunological increase in independent Inflammatory Inflammatory cytokines inflammatory storm Interaction involved lung tissues memory Migration Mild moderate mononuclear cell notable Patient PBMC Peripheral blood progression proliferation provide recruitment Result reveal risk S100A12 S100A8 S100A9 scRNA-seq dataset sequencing data severe COVID-19 Severe infection Severe patient severe patients severity of COVID-19 significantly single-cell single-cell sequencing subpopulation subset subtype supplementary material Symptom T cell understanding up-regulated [DOI] 10.1186/s13073-022-01021-1 PMC 바로가기 [Article Type] Research
Comparative analysis of antibody- and lipid-based multiplexing methods for single-cell RNA-seq단일 세포 RNA-seq에 대한 항체 및 지질 기반 다중화 방법의 비교 분석Comparative Study Published on 2022-02-162022-09-12 Journal: Genome Biology [Category] MERS, 진단, [키워드] 10x antibodies antibody approach batch effects Benchmarking Brain Cell cell line cell lines cells CITE-seq clinical sample Clinical studies Comparative analysis datasets demultiplexing Efficiency evaluated Genetic variation Genomics Hashing healthy human cell human PBMC indicate intrinsic Lipid lung MULTI-seq Multiplexing nuclei outperform PBMC protocol reagent reducing reduction Result RNA-Seq Sample multiplexing SARS-CoV-2 infected patients scRNA-seq Sequencing single-cell single-cell RNA-seq spleen Strains supplementary material tissue tissues [DOI] 10.1186/s13059-022-02628-8 PMC 바로가기 [Article Type] Comparative Study
SARS-CoV-2 Immunization Orchestrates the Amplification of IFNγ-Producing T Cell and NK Cell PersistenceImmunology Published on 2022-02-142022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] adjusted age amplification association B cell boost CD4 CD8 Cohort concerning confounder correlation COVID-19 death develop dose doses effective Effectiveness group groups HCW healthcare worker hematologic IgG immune protection immune response Immunity immunological protection intervals kinetic Lymphocytes mRNA BNT162b2 mRNA-based vaccine NK NK cell NK cells novel pandemic parameter PBMC Pfizer polymerase chain positive prime immunization protective immunity recipient responders Result risk SARS-CoV-2 SARS-COV-2 infection stimulated subset T cell T cells the vaccine two-dose regimen vaccination Vaccines virus [DOI] 10.3389/fimmu.2022.798813 PMC 바로가기 [Article Type] Immunology
T Cell Response following Anti-COVID-19 BNT162b2 Vaccination Is Maintained against the SARS-CoV-2 Omicron B.1.1.529 Variant of ConcernBrief Report Published on 2022-02-082022-10-28 Journal: Viruses [Category] COVID-19, COVID19(2023년), Fulltext, MERS, SARS, [키워드] addition Administered affected antigenic drift B.1.1.529 BNT162b2 carried Cell cells collected comparable concern COVID-19 COVID-19 pandemic dominant Efficacy eight ELISPOT examined fluorescent FluoroSpot form healthy volunteer IL-10 IL-4 Immunity individuals Maintained manifested mononuclear cell omicron overlapping PBMC peptide Peripheral blood progression Protective protective immunity reactivity reduced representing response SARS-CoV-2 SARS-CoV-2 virus variants secreting severe disease manifestations significantly Spike protein stimulated T cell T cell response T cell responses tested Th1 response the SARS-CoV-2 vaccination Vaccines variant variants of concern VoC wild-type Wuhan-1 [DOI] 10.3390/v14020347 PMC 바로가기 [Article Type] Brief Report
Children and Adults With Mild COVID-19: Dynamics of the Memory T Cell Response up to 10 Months경증 COVID-19가 있는 어린이 및 성인: 최대 10개월까지 기억 T 세포 반응의 역동성Clinical Trial Published on 2022-02-072022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 임상, 치료기술, 치료법, [키워드] activated Activation acute respiratory syndrome acute respiratory syndrome coronavirus Adaptive immunity Adults Analysis Antibody Response Antigen CD4 Cell cellular children Concentration concentrations contribute controls Coronavirus-2 correlated Course COVID-19 Cytokines detectable determine develop Dynamics effective effector memory ELISPOT exposure to frequencies heat heat-inactivated IFN-γ IFN-γ ELISPOT IgG immune memory infected children infections Longitudinal assessment marker memory T memory T cell Mild mild SARS-CoV-2 infection mild symptoms Mild-to-moderate moderate moderate symptoms Month Nucleocapsid proteins overlapping PBMC PBMCs peptide peptides performed Peripheral blood peripheral blood samples recall reduced Reinfection respiratory response Result SARS-CoV-2 SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV-2-specific T cell SARS-CoV-2-specific T cells serum antibody severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 significantly Stimulation symptom onset Symptoms T cell T cell immunity T cell response T cells Th1 phenotype [DOI] 10.3389/fimmu.2022.817876 PMC 바로가기 [Article Type] Clinical Trial
Effect of Fucoidan on the Mitochondrial Membrane Potential (ΔΨm) of Leukocytes from Patients with Active COVID-19 and Subjects That Recovered from SARS-CoV-2 InfectionArticle Published on 2022-01-242022-10-30 Journal: Marine Drugs [Category] COVID-19, SARS, [키워드] addition Anti-inflammatory Anti-viral cationic CCCP cellular homeostasis complementary COVID-19 COVID-19 patient determine Effect evaluated fucoidan healthy control healthy subject induce Infection mitochondrial membrane potential mononuclear cell PBMC Peripheral blood polysaccharide Potential Potential treatment promote recovered SARS-CoV-2 SARS-COV-2 infection subject subjects therapeutic target therapy Treatment [DOI] 10.3390/md20020099 PMC 바로가기 [Article Type] Article
Overview of Anti-SARS-CoV-2 Immune Response Six Months after BNT162b2 mRNA VaccineArticle Published on 2022-01-222022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] all subject Analysis antibody Antigen B-cell response BNT162b2 mRNA BNT162b2 mRNA vaccination BNT162b2 mRNA vaccine CD4 CD8 cellular immunity chemiluminescence circulating contrary evaluated FACS analysis fuzzy system Health care worker humoral immune response Humoral immunity IgG level immune response in vitro memory B-cell mimicking MONITOR Month Neutralization assay Neutralizing antibodies neutralizing antibody PBMC Prospective Study Protein Reinfection response SARS-CoV-2 serum samples significant decrease spike subject subjects subset T-cell tested Th17 cells trimeric vaccination Variability [DOI] 10.3390/vaccines10020171 PMC 바로가기 [Article Type] Article
The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19β-NGF/TrkA 신호 전달 경로는 회복기 COVID-19에서 항핵단백질 IgG의 생산과 관련이 있습니다Immunology Published on 2022-01-142022-09-12 Journal: Frontiers in Immunology [Category] 진단, [키워드] β-NGF age analysed antibody antibody production Asymptomatic CD4 convalescent correlated COVID-19 Cytokines defined discrepancy ELISPOT expressed Factor First wave flow cytometry high variability Host IC50 IFN-γ IgG IgG level IL-1α Infection Inflammation mild SARS-CoV-2 infection molecular mechanism neutralising antibody pandemic pathway PBMC PCR-confirmed peptides positive positive correlation Production receptor Regulation reported Result SARS-CoV-2 SARS-COV-2 infection selected serum severe COVID-19 shown significantly specific antibody subject subjects T cell T cells titre undetectable virus-specific T cell Volunteer was determined were measured while [DOI] 10.3389/fimmu.2021.813300 PMC 바로가기 [Article Type] Immunology
Communication Pattern Changes Along With Declined IGF1 of Immune Cells in COVID-19 Patients During Disease Progression질병 진행 중 COVID-19 환자의 면역 세포 IGF1 감소와 함께 의사 소통 패턴 변화Immunology Published on 2022-01-142022-09-11 Journal: Frontiers in Immunology [Category] 치료기술, 치료제, [키워드] activated acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis analyzed cause Cell change changed chemokine chemokines communication communication pattern Contact coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 Crosstalk Cytokines described feature growth factor IGF1 Immune cell immune cells immune response immune system Inflammatory Inflammatory cytokine Inflammatory cytokines insulin-like growth factor less mononuclear cell Most patient Most patients Negative conversion pandemic Patient pattern PBMC Peripheral blood Regulatory respiratory reveal SARS-CoV-2 SARS-CoV-2 RNA Serum level serum levels severe acute respiratory syndrome Coronavirus Signal transduction single-cell sequencing stages status Symptoms T cell T cells therapeutic target therapeutic targets [DOI] 10.3389/fimmu.2021.729990 PMC 바로가기 [Article Type] Immunology