Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015Short Communication Published on 2019-11-082022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] caused characterized clade clinical isolates collected coronavirus CoV Deletion eight exhibited full length Genome genomic sequences growth growth kinetics Host HUH7 cells Human human infection independent isolate Lineage MERS MERS-CoV MERS-CoV genome Middle East Middle East respiratory syndrome human coronavirus mutant ORF1a ORF3 Patient performed Phylogenetic analysis Phylogeny plasticity Region replicated reported Saudi Arabia Sequencing specimen specimens strain syndrome Vero Vero cells viral isolates viral respiratory zoonotic [DOI] 10.1099/jgv.0.001334 PMC 바로가기 [Article Type] Short Communication
Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strainResearch Published on 2019-10-282022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] amino acid change Antigen Attenuated vaccines attenuation caused Cell characterization Clinical symptoms cytopathic effects Epidemic epidemic diarrhea experiment Genome histopathological intestinal lesions passage pathogenicity PEDV Porcine epidemic diarrhea virus protective effect reductions in responsible Result Sequence analysis Strains survival rate vaccine strain variant Vero Vero Cell viral titer virulence virulent virus virus strain was obtained whole-genome sequencing [DOI] 10.1186/s12985-019-1232-7 PMC 바로가기 [Article Type] Research
Porcine epidemic diarrhea virus S1 protein is the critical inducer of apoptosisResearch Published on 2018-11-072022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] accompanied Activation age AIFM1 analyzed Apoptosis Apoptosis-inducing factor mitochondria associated 1 (AIFM1) caspase-3 caspase-8 caspase-9 caused Cell cell membrane characterized cleavage Concentration contagious contribute coronavirus Coronavirus infection Critical cytopathic effects demonstrated diarrhea disease effective epidemic diarrhea expression flow cytometry functional protein high mortality host cell host cells IBV immunofluorescence assay in vitro in vivo induce inducer infect infected cell infected cells inhibit less MERS molecular non-structural protein Nsp1–16 nuclear ORF3 overexpressing overexpression PARP PEDV Plasmid porcine epidemic diarrhea virus (PEDV) Protein Result S1 and S2 S1 protein spike spike S1 protein structural protein supplementary material syncytium formation tested TGEV the S1 protein transfected cells Vero VERO-E6 Vero-E6 cell virus was measured watery wild-type strain [DOI] 10.1186/s12985-018-1078-4 PMC 바로가기 [Article Type] Research
Establishment of porcine enterocyte/myofibroblast co-cultures for the growth of porcine rota- and coronavirusesArticle Published on 2018-10-122022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] bovine can be used Cell Characteristics co-culture co-cultured contributing to coronavirus Culture demonstrated enteric virus Enterocyte epithelial cells evaluated Extracellular matrix fecal fetal Genotype growth growth factor infect Last Miller PEDV proliferation Replication rotavirus strain secrete serum strain subepithelial susceptibility susceptible TGEV Vero viruses [DOI] 10.1038/s41598-018-33305-1 PMC 바로가기 [Article Type] Article
TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero CellsArticle Published on 2017-05-182022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] absence Activation cleaved co-expression Coronavirus infections DESC1 Effects epidemic diarrhea exhibited exogenous trypsin expressing facilitate facilitated host proteases human coronaviruses in vitro Influenza Interaction involved isolate MSPL PEDV Porcine epidemic diarrhea virus progression Replication S protein Spread Stage tested the S protein TMPRSS2 transmembrane serine protease TTSP TTSPs Type type II transmembrane serine protease Vero Vero Cell Vero cells virus virus virus production virus replication [DOI] 10.3390/v9050114 PMC 바로가기 [Article Type] Article
Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturingArticle Published on 2014-11-122023-06-25 Journal: Vaccine [Category] 신종인플루엔자, [키워드] Antigenic stability Genetic stability influenza vaccines MDCK Vaccine-certified cell lines Vero virus isolation Virus purification. [DOI] 10.1016/j.vaccine.2014.06.045 PMC 바로가기
Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cellsResearch Article Published on 2011-08-112023-05-22 Journal: BMC Biotechnology [Category] Fulltext, 신종인플루엔자, [키워드] bioreactor Influenza microcarrier NS1 Vero [DOI] 10.1186/1472-6750-11-81 PMC 바로가기 [Article Type] Research Article
Acquisition of Cell–Cell Fusion Activity by Amino Acid Substitutions in Spike Protein Determines the Infectivity of a Coronavirus in Cultured CellsResearch Article Published on 2009-07-022022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acid acquisition activity Amino acid bronchitis Cell cell line cells chimeric cloned compensatory mutation coronavirus cultured cell cultured cells determine determined ENhance expression fusion heptad repeat Host host cell IBV infectious Infectivity Interaction introduced kidney membrane fusion Mutation Point mutation Protein Region required responsible S gene S protein S1 domain shown site-directed mutagenesis specificity spike strain Substitution syncytia formation the S protein variant Vero Vero cells viral spike [DOI] 10.1371/journal.pone.0006130 PMC 바로가기 [Article Type] Research Article
G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cellsShort Report Published on 2005-08-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] amino acids Analysis Annexin Apoptosis apoptosis induction Atypical Cell cell cycle arrest cells apoptosis characterized coronavirus COS-7 cell COS-7 cells ENCODE expression found fusion protein Genome immune induce infect labeling life-threatening liver lung mechanism Necrosis non-structural protein non-structural proteins organ organs over-expression Pathogenesis Pneumonia predicted Protein Proteins related Replication respiratory SARS pathogenesis SARS-CoV severe similarity structural protein Till transfected cell Vero virulence viruses [DOI] 10.1186/1743-422X-2-66 PMC 바로가기 [Article Type] Short Report