Head-to-Head Evaluation of Five Automated SARS-CoV-2 Serology Immunoassays in Various Prevalence SettingsArticle Published on 2021-04-102022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] Accuracy Analysis Anti-N anti-S anti-SARS-CoV-2 AUC automated characteristic COVID-19 COVID-19 sera diagnostic DiaSorin displaying electrochemiluminescent immunoassay (ECLIA) Enzyme-linked immunosorbent assay (ELISA) epitope Euroimmun FIVE highest immunoassays negative control Negative predictive value NPV performed population-based cohort Positive predictive value PPV Prevalence Probability reference method ROC Roche S-RBD S1/S2 SARS-CoV-2 screening test selected sensitivity sera serology setting specificity [DOI] 10.3390/jcm10081605 PMC 바로가기 [Article Type] Article
A human coronavirus evolves antigenically to escape antibody immunity인간 코로나 바이러스는 항체 면역을 피하기 위해 항원 적으로 진화합니다.Research Article Published on 2021-04-082022-08-13 Journal: PLoS Pathogens [Category] Biology and Life Sciences, COVID-19, SARS, [키워드] 229E 229E strain 229E strains 229E viral strain 229E viruses accumulate anti-SARS-CoV-2 titer anti-SARS-CoV-2 titers antibody antibody immunity antigenic evolution common-cold comparable coronavirus coronavirus 229E coronavirus vaccine Coronavirus vaccines Coronaviruses degrade elicited Evolution Hope human coronavirus human coronavirus 229E human sera Hypothesis identify Immunity in some individuals Infection less Mutation neutralization neutralize Neutralizing titer neutralizing titers NTD occur Older other coronaviruses pace PROTECT question re-infect Re-infection Re-infections Receptor-binding domain Region SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccines seasonal coronavirus sera Spike protein strain Strains subsequent the RBD the receptor-binding domain titers vaccination variants of SARS-CoV-2 Viral viral evolution viral mutation Viral mutations viral spike viral strains virus viruses was collected [DOI] 10.1371/journal.ppat.1009453 PMC 바로가기 [Article Type] Research Article
Evaluation of a Broad Panel of SARS-CoV-2 Serological Tests for Diagnostic UseArticle Published on 2021-04-082022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] Antigen Clinical use Combination convalescent Corona COVID-19 ECLIA ELISA ELISAs enrolled evaluate IgG immune response Infection less LFA naïve subject neutralization nucleocapsid protein pandemic Panel platform respiratory Result SARS-CoV-2 sensitivity sera serological Serological immune response Serological tests Spike protein Test tested Vaccine-induced immunity [DOI] 10.3390/jcm10081580 PMC 바로가기 [Article Type] Article
IgG and IgM antibody formation to spike and nucleocapsid proteins in COVID-19 characterized by multiplex immunoblot assays다중 면역블롯 분석을 특징으로 하는 COVID-19에서 스파이크 및 뉴클레오캡시드 단백질에 대한 IgG 및 IgM 항체 형성Research Article Published on 2021-04-072022-09-01 Journal: BMC Infectious Diseases [Category] MERS, 진단, 치료법, 치료제, [키워드] allergy antibody antibody formation Antibody Response Antibody responses Antigen Antisera assays autoimmune condition autoimmune conditions caused characterized COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 patients criteria detection diagnostic tests Diseases IgG IgG and IgM IgM IgM antibodies IgM antibody immunoblot Influenza Laboratory less Line immunoblot assay mild symptoms multiplex Multiplex assay N proteins nucleocapsid protein offer performed plateau positive Positive test proportion proportions Proteins qPCR test Rapid RBD Receptor binding domain Receptor-binding domain recombinant Recombinant nucleocapsid protein recommendation Result RT-qPCR RT-qPCR test SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 virus SARS-CoV-2 virus protein SARS-CoV-2 virus proteins sensitivity sera Seroconversion serological Serological assay Serological diagnosis Specificities specificity Spike protein spike protein of SARS-CoV-2 supplementary material the spike protein Viral viral infections were used [DOI] 10.1186/s12879-021-06031-9 PMC 바로가기 [Article Type] Research Article
Large-scale IgM and IgG SARS-CoV-2 serological screening among healthcare workers with a low infection prevalence based on nasopharyngeal swab tests in an Italian university hospital: Perspectives for public health이탈리아 대학 병원의 비인두 면봉 검사를 기반으로 한 감염 유병률이 낮은 의료 종사자를 대상으로 한 대규모 IgM 및 IgG SARS-CoV-2 혈청 검사: 공중 보건에 대한 전망Article Published on 2021-04-012022-09-11 Journal: Environmental research [Category] MERS, SARS, 진단, 치료기술, [키워드] Accuracy association Asymptomatic asymptomatic patient asymptomatic patients chemiluminescence immunoassay close contact Cohort contact with COVID-19 COVID-19 patients Decision-making demonstrated determine Diagnosis diagnosis of COVID-19 Early detection HCW HCWs healthcare worker Healthcare workers hospital IgG and IgM IgM and IgG infection prevalence Italian large cohort Nasopharyngeal swab nasopharyngeal swab test observation period Patient positive positive COVID-19 Prevalence public health risk Risk assessment RT-PCR SARS-CoV-2 SARS-COV-2 infection screened sensitivity sera serological Serological assay Serological screening serological test Serological tests specificity Swab test Symptomatic patients Viral Viral load viral spread widespread with COVID-19 [DOI] 10.1016/j.envres.2021.110793 PMC 바로가기 [Article Type] Article
Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques회복기 COVID-19 환자 및 RBD 나노입자 백신 히말라야 원숭이로부터 수집된 혈청에 의한 신규 돌연변이 B.1.1.7 및 501Y.V2를 중화하기 위한 차등 효율Article Published on 2021-04-012022-09-11 Journal: Cellular and Molecular Immunology [Category] MERS, SARS, 변종, 진단, [키워드] 501Y.V2 B.1.1.7 collected convalescent COVID-19 patient Efficiency mutant neutralize RBD rhesus macaque sera [DOI] 10.1038/s41423-021-00641-8 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 PatientsPlant Science Published on 2021-03-312022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] acute respiratory syndrome addition African antibody Antibody Response assays can be used confirmed case convalescent coronavirus Coronavirus disease 2019 correlated COVID-19 death detect detectable develop Developing Diagnosis disease domain ELISA expressed humoral responses IgA IgG IgM responses Immunoglobulin in-house ELISA infected patient majority median Mild Moderate COVID-19 Nicotiana benthamiana optical pandemic participant Participants Patient PCR plant plant expression plasma positive produced protective immunity ranged RBD reactivity response robust Saliva Sample SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2-specific IgG sensitivity sera Serological assay serology significantly South specificity tested the spike protein Viral proteins volunteers [DOI] 10.3389/fpls.2021.589940 PMC 바로가기 [Article Type] Plant Science
Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies Research Published on 2021-03-262022-10-05 Journal: Nature Medicine [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome antibody B.1.1.7 B.1.351 B.1.351 variant B1.351 circulating collected convalescent convalescent individual convalescent sera coronavirus D614G examined gene encoding GFP immune evasion immunized individual individuals infected individuals Infection less Mutation nasal swab natural infection Neutralization assay neutralized neutralizing antibody neutralizing titers Pfizer positive raise reduction in remained reporter cell risk of infection SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 B.1.1.7 SARS-CoV-2 variant sensitivity sera Serological assay South Africa Spike protein spread to strain Symptoms tested the spike protein United Kingdom vaccinated individual vaccination vaccine dose vaccinees variant virus [DOI] 10.1038/s41591-021-01318-5 [Article Type] Research
mRNA vaccination boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infectionReport Published on 2021-03-252022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, [키워드] antibody B.1.351 B.1.351 variants boost collected cross-neutralizing Donor elicit elicited examined highlight immunization Infection mRNA vaccination mRNA vaccines neutralize neutralized Neutralizing antibodies neutralizing antibody Neutralizing titer raised Receptor binding domain responses SARS-CoV-1 SARS-COV-2 infection SARS-CoV-2 variant sera uninfected vaccination variant Wuhan-Hu-1 [DOI] 10.1126/science.abg9175 PMC 바로가기 [Article Type] Report
SARS-CoV-2 Proteome-Wide Analysis Revealed Significant Epitope Signatures in COVID-19 PatientsSARS-CoV-2 Proteome-Wide Analysis, COVID-19 환자에서 중요한 에피토프 서명 밝혀Clinical Trial Published on 2021-03-232022-08-13 Journal: Frontiers in Immunology [Category] SARS, 임상, 진단, [키워드] acute respiratory disease acute respiratory distress Antibody Response approach asymptomatic infection B cells causative agent China clinically coronavirus COVID-19 COVID-19 outbreak COVID-19 patient COVID-19 patients death diagnostic disease epitope epitope identification exposure to high-density Host immune response humoral immune response Humoral response humoral responses IgG immune responses International Intervention leads Longevity marker Mild outcome peptide peptide microarray Predictive prognostic proteome public health emergency respiratory distress respiratory tract infection Revealed SARS-CoV-2 screened sera severe disease severe pneumonia signature Significant understanding Viral viral infection viral infections WHO [DOI] 10.3389/fimmu.2021.629185 PMC 바로가기 [Article Type] Clinical Trial