Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoVResearch article Published on 2009-12-032022-10-27 Journal: BMC Immunology [Category] Coronavirus, [키워드] acute respiratory syndrome analyzed assays autologous binding Candidates Cell-mediated immune responses cleavage conserved coronavirus CoV CTL dendritic cell different Donor eight epitope Ex vivo full-length S protein glycoprotein healthy help identification IFN-γ immunized immunized mice immunopathology lysed mechanism Pathogenesis PBL peptide peptides Plasmid predict processed Protein Region released Result S protein SARS-CoV SARS-CoV infection sequence target cell tested the S protein the spike protein transgenic mice virus virus clearance [DOI] 10.1186/1471-2172-10-61 PMC 바로가기 [Article Type] Research article
Acquisition of Cell–Cell Fusion Activity by Amino Acid Substitutions in Spike Protein Determines the Infectivity of a Coronavirus in Cultured CellsResearch Article Published on 2009-07-022022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acid acquisition activity Amino acid bronchitis Cell cell line cells chimeric cloned compensatory mutation coronavirus cultured cell cultured cells determine determined ENhance expression fusion heptad repeat Host host cell IBV infectious Infectivity Interaction introduced kidney membrane fusion Mutation Point mutation Protein Region required responsible S gene S protein S1 domain shown site-directed mutagenesis specificity spike strain Substitution syncytia formation the S protein variant Vero Vero cells viral spike [DOI] 10.1371/journal.pone.0006130 PMC 바로가기 [Article Type] Research Article
Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2Animal Published on 2008-11-012022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] ACE-2 Angiotensin converting enzyme-2 assays binding Cell consequence Contact coronavirus coronavirus spike protein different enzyme explain groups host cell Infection Interaction investigated mild respiratory NL63 Protein receptor required residue S protein SARS-CoV SARS-CoV S protein Severe respiratory distress virus [DOI] 10.1099/vir.0.2008/003962-0 PMC 바로가기 [Article Type] Animal
Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic CellsResearch Article Published on 2008-07-162022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] activated activity Antibody Response baculovirus baculovirus bat CD4+ T cell CD40 CD80 CD83 CD86 co-stimulatory molecule compared CoV cytokine Cytokines DC DCs demonstrated dendritic cell different DNA DNA vaccine evidenced Express expression finding Human IL-10 IL-6 immature incorporation indicated information inoculated insect cell labeling led to M protein membrane membrane protein mice Microscopy origin Particle Pathogenesis Population Protein response S protein SARS Coronavirus SARS CoV SARS-CoV SARS-CoV S SARS-like coronavirus secretion SL-CoV stimulate Stimulating T cell response the S protein TNF-α up-regulated Vaccine development Virus-like particle VLP VLPs western blot [DOI] 10.1371/journal.pone.0002685 PMC 바로가기 [Article Type] Research Article
Adaptive evolution of the spike gene of SARS coronavirus: changes in positively selected sites in different epidemic groupsResearch Article Published on 2006-10-042022-10-28 Journal: BMC Microbiology [Category] Coronavirus, [키워드] acute respiratory syndrome adaptive addition Amino acid changes in characterized contribute coronavirus Course CoV detected different domain Epidemic epidemiological Evolution found greater group groups heptad repeat hosts human host humans membrane fusion occurred outbreak Phylogenetic tree positive Positive selection proportion Protein RBD receptor residue Result reveal S gene S protein SARS-CoV selected selective selective pressure selective sweep spike gene strain subsequent the S protein Transmission two group two groups Variation viral adaptation [DOI] 10.1186/1471-2180-6-88 PMC 바로가기 [Article Type] Research Article
Potential antivirals and antiviral strategies against SARS coronavirus infectionsReview Published on 2006-01-102023-06-16 Journal: Expert review of anti-infective therapy [Category] MERS, [키워드] coronavirus interferon monoclonal antibody protease inhibitors S protein SARS SARS-CoV inhibitors siRNA [DOI] 10.1586/14787210.4.2.291 PMC 바로가기 [Article Type] Review
SARS Vaccine DevelopmentSynopsis Published on 2005-07-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome can be used Clinical use conformational coronavirus develop Developing development effective inactivated inducer Infection Neutralizing antibodies Neutralizing epitone neutralizing epitopes Prevent Protein RBD Receptor-binding domain Recombinant protein S protein Safe SARS SARS-CoV SARS-CoV vaccine sequence Spike protein the RBD the receptor-binding domain the S1 subunit Vaccine Vaccines vector [DOI] 10.3201/eid1107.050219 PMC 바로가기 [Article Type] Synopsis
SARS Antibody Test for SerosurveillanceResearch Published on 2004-09-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome Blood can be used characterized coronavirus detected disease disease transmission Donor ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay epitope mapping healthcare worker healthy helping hospital identify immunoassay Infection M protein membrane N protein nosocomial nucleocapsid protein onset of fever Pathogens peptide peptide-based remained Research retrospective Retrospective study S protein SARS patients SARS-CoV sensitivity Sensitivity and specificity sequence Seroconversion serologic test serum sample specificity Standardization synthetic peptide Taiwan Test treated was used [DOI] 10.3201/eid1009.040101 PMC 바로가기 [Article Type] Research
A conditional-lethal murine coronavirus mutant that fails to incorporate the spike glycoprotein into assembled virionsArticle Published on 2000-03-072022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Analysis Cell coronavirus found host cell receptor Infected membrane fusion MHV MHV-A59 mouse hepatitis virus murine mutant phenotype Point mutation receptor responsible resulting S glycoprotein S protein Serine shown spike glycoprotein syncytia formation temperature Temperature-sensitive mutant virion virus virus assembly wild type [DOI] 10.1016/0168-1702(95)00100-X PMC 바로가기 [Article Type] Article