Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacitiesSARS-CoV-2에 대한 항체 동형 다양성은 차등 혈청 중화 능력과 관련이 있습니다Article Published on 2021-03-102022-09-01 Journal: Scientific Reports [Category] SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] addressed Analysis antibodies antibody antibody isotype antibody isotypes Antibody Response Antibody responses antigens authentic SARS-CoV-2 virus can be used Cohort Containment measure containment measures contribute convalescent patient Convalescent patients COVID-19 pandemic cross ELISA Evidence healthcare worker IgA antibodies IgA antibody IgG IgM individual majority neutralization neutralization capacity Neutralizing Neutralizing antibody response Neutralizing titer nucleoprotein overcome performed positive predict preliminary evidence provide Pseudotyped virus recent Receptor binding domain SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 neutralization SARS-CoV-2 virus sera serum serum IgG Serum Neutralization spike Still sustained the SARS-CoV-2 the Spike understanding [DOI] 10.1038/s41598-021-84913-3 PMC 바로가기 [Article Type] Article
Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2ACE2 및 TMPRSS2의 안정적인 발현을 갖는 293T 세포를 사용하여 잘 특성화된 SARS-CoV-2 렌티바이러스 슈도바이러스 중화 분석의 확립Research Article Published on 2021-03-102022-08-13 Journal: PLoS ONE [Category] Biochemistry, SARS, 신약개발, 진단, [키워드] 293T 293T cell ACE2 angiotensin angiotensin converting enzyme assays cell line condition effort enzyme evaluated expressing expression generate Genetic highly pathogenic human angiotensin converting enzyme 2 lentiviral limitations neutralization neutralization titer neutralization titers Neutralizing antibodies neutralizing antibody plasma platform Precision pseudovirus Pseudovirus neutralization assay pseudoviruses reported SARS-CoV-2 SARS-CoV-2 entry scalability sera Serine stability target cells TMPRSS2 transmembrane serine protease transmembrane serine protease 2 variety virus viruses [DOI] 10.1371/journal.pone.0248348 PMC 바로가기 [Article Type] Research Article
Use of convalescent serum reduces severity of COVID-19 in nonhuman primates회복기 혈청의 사용은 비인간 영장류에서 COVID-19의 심각성을 감소시킵니다Article Published on 2021-03-092022-09-12 Journal: Cell Reports [Category] MERS, SARS, 비임상, 진단, [키워드] acute respiratory syndrome African green monkey anti-SARS-CoV-2 antibodies antibody Clinical efficacy Coagulopathy controls convalescent convalescent plasma Convalescent plasma therapy convalescent sera convalescent serum coronavirus coronavirus disease COVID-19 difference Diseases Donor early stage effective group Human in viral Inflammatory Lung pathology neutralizing antibody titers Neutralizing titer nonhuman primate Passive pathology Patient provided receive reduce reduced reductions in SARS-CoV-2 sera serum severity severity of COVID-19 the disease therapeutic transfer treat Trial Viral pneumonia [DOI] 10.1016/j.celrep.2021.108837 PMC 바로가기 [Article Type] Article
Antibody resistance of SARS-CoV-2 variants B.1.351 and B.1.1.7 Research Published on 2021-03-082022-10-05 Journal: Nature [Category] SARS, 변종, [키워드] B.1.1.7 B.1.351 B.1.351 variant combination therapy convalescent plasma COVID-19 COVID-19 pandemic E484K Effect Efficacy Emergency use globe individual initial Intervention monoclonal antibody Mutation N-terminal domain neutralization pandemic plasma Protective receptor-binding motif SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 virus sera shown South Spread the receptor-binding domain the spike protein therapy Transmission Treatment vaccine construct Vaccines widespread wild-type SARS-CoV-2 [DOI] 10.1038/s41586-021-03398-2 [Article Type] Research
Antibodies to neutralising epitopes synergistically block the interaction of the receptor‐binding domain of SARS‐CoV‐2 to ACE 2Original Article Published on 2021-03-072022-10-31 Journal: Clinical & Translational Immunology [Category] COVID-19, [키워드] ACE ACE‐2 ACE2 African antibody Antibody Response antigenic approach asked B‐cell binding blocked Blood Contact convalescent patient Convalescent patients COVID‐19 defined disease effective enzyme epitope exacerbate Identified identify immune response immunogens immunopathology induce Interaction memory B cells mice neutralising neutralising antibody objective overlapping peptide peptide epitopes peptides plasma Prevent Protein raised RBD receptor‐binding domain receptor‐binding domain reduced risk Region Result safety profile SARS‐CoV‐2 SARS‐CoV‐2‐specific antibody SARS‐CoV‐2 sera significantly South specificity Strains T‐cell responses tested tetramer staining the RBD the Spike the vaccine vaccinate Vaccine Vaccine development [DOI] 10.1002/cti2.1260 PMC 바로가기 [Article Type] Original Article
The Potential of Developing Pan-Coronaviral Antibodies to Spike Peptides in Convalescent COVID-19 Patients회복기 COVID-19 환자의 펩티드 급증을 위한 범 코로나바이러스 항체 개발의 가능성Article Published on 2021-03-062022-09-12 Journal: Archivum immunologiae et therapiae experimentalis [Category] SARS, 진단, [키워드] antibodies bind binding Blood cell entry cellular receptor collected collected data control group control patients convalescent COVID-19 patient Coronaviral coronavirus COVID-19 COVID-9 Cross reactivity cross-reactive antibody cross-reactivity decrease described Developing domain effective ELISA help heptad repeat hidden homology IgG antibody immunogenic immunoglobulins Infection infections inhibitory membrane membrane fusion MERS-CoV Middle East non-infected opposite peptide polyclonal antibody Population Potential presence of antibody raise reactivity recognize respiratory syndrome coronavirus S1 subunit S2 subunit SARS-CoV-2 SARS-COV-2 infection sera seropositive severity similarity spike Spike protein tested the spike protein Vaccine Viral mutations were used [DOI] 10.1007/s00005-021-00607-8 PMC 바로가기 [Article Type] Article
The effect of spike mutations on SARS-CoV-2 neutralizationSARS-CoV-2 중화에 대한 스파이크 돌연변이의 영향Report Published on 2021-03-062022-09-11 Journal: Cell Reports [Category] SARS, 변종, 진단, [키워드] Abstract active against acute respiratory syndrome acute respiratory syndrome coronavirus Alter Amino acid amino acid changes amino acid substitution amino acids antibodies antibody Antibody Response Antibody responses B.1.1.7 B.1.1.7 variant B.1.351 change changes in collected convalescent sera coronavirus Critical decrease Divergence effective Efficacy ENCODE evaluate highlight Immune escape individual Infection less lineages mild illness monoclonal antibody Multiple mutants mutated Mutation mutations neutralization Neutralizing antibodies neutralizing antibody new SARS-CoV-2 P.1 polyclonal antibodies polyclonal antibody potency produced Protective Protein pseudotype Pseudotyped virus Real-time monitoring recognizing reduced respiratory SARS-CoV SARS-CoV-2 SARS-CoV-2 neutralization SARS-CoV-2 pseudotyped virus SARS-CoV-2 spike SARS-CoV-2 variants SARS-CoV-2 virus sensitivity sera serology serum sample serum samples severe acute respiratory syndrome Coronavirus spike mutation Substitution Vaccine vaccine efficacy variant variant spike Variation Viral viral entry [DOI] 10.1016/j.celrep.2021.108890 PMC 바로가기 [Article Type] Report
Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan일본에서 N 및 S 단백질을 모두 검출하는 새로운 자동화된 화학발광 항-SARS-CoV-2 IgM 및 IgG 항체 분석 시스템의 검증Research Article Published on 2021-03-042022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 anti-SARS-CoV-2 IgM antibody Antibody test autoantibodies autoantibody automated CLIA analyzer collected complement concordance rate coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cutoff value detect Diagnosis evaluated False-positive hospital IgG IgG antibody IgG titers IgM IgM and IgG IgM/IgG investigated Japan Laboratory testing limitations linearity methodology N and S proteins non-COVID-19 patient patients PCR PCR method PCR test PCR-negative PCR-positive PCR-positive COVID-19 patients Precision Randomly repeatability respiratory S protein SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 IgM selected sera Seroconversion serum antibodies serum antibody serum sample serum samples severe acute respiratory syndrome Coronavirus validation [DOI] 10.1371/journal.pone.0247711 PMC 바로가기 [Article Type] Research Article
Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies Research Published on 2021-03-042022-10-05 Journal: Nature Medicine [Category] SARS, 변종, [키워드] acute respiratory syndrome African antibodies binding antibody Antibody neutralization B.1.1.7 isolate BNT162b2 mRNA vaccine caused chimeric conserved convalescent sera coronavirus COVID-19 pandemic E484K human convalescent sera human sera immune in vitro in vivo inhibitory activity introduced mAb mAb cocktail mAbs monoclonal monoclonal antibody mRNA N-terminal domain neutralization neutralization potency Neutralizing polyclonal antibody Prevent recipient reduced regions resistance SARS-CoV-2 SARS-CoV-2 variant sequence sera South spike gene spike mutation spike receptor-binding domain strain the receptor-binding domain Vaccine variants Viral variants virus [DOI] 10.1038/s41591-021-01294-w [Article Type] Research
Automated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies항-SARS-CoV-2 혈청 항체의 자동화된 웨스턴 면역블롯팅 검출Comparative Study Published on 2021-03-032022-09-10 Journal: European Journal of Clinical Microbiology & Infect [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] anti-SARS-CoV-2 Antigen automated Automated Western immunoblotting chemiluminescence collected complementary COVID-19 COVID-19 patients cross-reactivity diagnosed ELISA ELISA kit exhibited HCoVs IgA IgG IgM Immunoglobulin immunoglobulins inactivated Jess MONITOR nucleocapsid onset of symptoms other coronaviruses Patient reactivity RT-PCR SARS-CoV-2 SARS-CoV-2 antigens SARS-CoV-2 IgG SARS-CoV-2 lineage sensitivity sera serologic assay Serological assay serology serum antibody simple specificity Spike protein subset supplementary material tested the spike protein was used [DOI] 10.1007/s10096-021-04203-8 PMC 바로가기 [Article Type] Comparative Study