Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic CellsResearch Article Published on 2008-07-162022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] activated activity Antibody Response baculovirus baculovirus bat CD4+ T cell CD40 CD80 CD83 CD86 co-stimulatory molecule compared CoV cytokine Cytokines DC DCs demonstrated dendritic cell different DNA DNA vaccine evidenced Express expression finding Human IL-10 IL-6 immature incorporation indicated information inoculated insect cell labeling led to M protein membrane membrane protein mice Microscopy origin Particle Pathogenesis Population Protein response S protein SARS Coronavirus SARS CoV SARS-CoV SARS-CoV S SARS-like coronavirus secretion SL-CoV stimulate Stimulating T cell response the S protein TNF-α up-regulated Vaccine development Virus-like particle VLP VLPs western blot [DOI] 10.1371/journal.pone.0002685 PMC 바로가기 [Article Type] Research Article
Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 ActivationResearch Article Published on 2008-06-132022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] Activation ADP-ribosylation Analysis ARF ARF1 association Author cells cellular cellular pathway characteristic complex connection coronavirus coronavirus Coronavirus replication Critical demonstrated dominant-negative downstream Endoplasmic reticulum Express expression Factors GBF1 GTPase GTPases guanine Host Human induce infected cell Infection inhibited inhibiting interfere involved knockdown MDCK cells membrane MHV Microscopy mouse mouse hepatitis mutant nucleotide observation provide Quantitative reduced Replication replication complex required RNA RNA virus secretory pathway significantly siRNA siRNAs structures target vesicles viruses [DOI] 10.1371/journal.ppat.1000088 PMC 바로가기 [Article Type] Research Article
SARS-Coronavirus Replication/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In VitroResearch Article Published on 2008-05-022022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] activity acute respiratory syndrome addition Author cause centrifugation characteristic complex complexes connection Critical cytoplasm cytoplasmic Degradation dependent on develop digestion dissect eight enzyme Enzymes Factor fraction functional genomic RNA Host host cell membranes host cells in vitro in vitro activity Infected infected cell life-threatening membrane membrane structure membrane structures membranes non-structural protein NSP Nsps polyproteins precursor produced protease PROTECT protein synthesis released remained replicate Replication required RNA RNA synthesis robust RTC SARS-coronavirus SARS-CoV SARS-CoV-infected cells Structure subgenomic mRNA subgenomic mRNA synthesis subunit susceptible Transcription Treatment viral genome replication Viral RNA viral RNA synthesis virus-induced membrane structure virus-induced membrane structures [DOI] 10.1371/journal.ppat.1000054 PMC 바로가기 [Article Type] Research Article
Inhibition of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infectivity by peptides analogous to the viral spike proteinArticle Published on 2006-04-172022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] affected Anti-viral antiviral activity Asia Atypical cell cytotoxicity Class I viral fusion protein Concentration concentrations coronavirus CoV domain dose-dependent Effect Europe fusion peptide fusion protein glycoprotein heptad repeat hydrophobicity IC50 identify inhibited inhibition inhibitor membrane membrane fusion MHV murine hepatitis virus N-terminus North America peptide Peptide inhibitor Pneumonia provide recent Receptor binding Region regions responsible S2 subunit SARS-CoV severe Severe acute respiratory syndrome-associated coronavirus six-helix bundle tested the S protein viral infectivity viral spike viral spike protein was used white [DOI] 10.1016/j.virusres.2006.03.001 PMC 바로가기 [Article Type] Article
Rapid and sensitive detection of multiple genes from the SARS‐Coronavirus using quantitative RT‐PCR with dual systemsResearch Article Published on 2005-08-242022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] 2003 ABI accurate acute respiratory syndrome addition Applied Biosystem caused Cell center comparable Control coronavirus cross demonstrated described detect detection detection efficiency Diagnosis evaluated examined Gene human sera in vitro Inc Infection isolate membrane nucleocapsid other coronavirus outbreak PCR prevention primer Prism Quantitative Rapid reagent real‐time quantitative RT‐PCR relative sensitivity RNA Roche RT‐PCR Safe SARS SARS patients SARS‐CoV structural genes SARS2 sensitivity sequence specimen specimens System TaqMan assay transcribed Viral RNA virus viruses [DOI] 10.1002/jmv.20432 PMC 바로가기 [Article Type] Research Article
SARS Antibody Test for SerosurveillanceResearch Published on 2004-09-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome Blood can be used characterized coronavirus detected disease disease transmission Donor ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay epitope mapping healthcare worker healthy helping hospital identify immunoassay Infection M protein membrane N protein nosocomial nucleocapsid protein onset of fever Pathogens peptide peptide-based remained Research retrospective Retrospective study S protein SARS patients SARS-CoV sensitivity Sensitivity and specificity sequence Seroconversion serologic test serum sample specificity Standardization synthetic peptide Taiwan Test treated was used [DOI] 10.3201/eid1009.040101 PMC 바로가기 [Article Type] Research
Coronavirus IBV: Partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41Article Published on 2002-11-122022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Amino acid Analysis blocked bronchitis chymotrypsin cleavage cleavage site coronavirus coronavirus IBV determined Frame IBV identify in vivo infectious leucine M41 membrane N-terminal N-terminus nucleocapsid nucleotide sequence Partial peptide performed precursor propolypeptide protein sequencing related removed residue S1 and S2 sequence Sequencing Serine spike Spike protein strain trypsin Vero Cell Virus spike [DOI] 10.1016/0168-1702(86)90037-7 PMC 바로가기 [Article Type] Article