Attenuation of Infectious Bronchitis Virus in Eggs Results in Different Patterns of Genomic Variation across Multiple ReplicatesGenetic Diversity and Evolution Published on 2019-05-012022-10-30 Journal: Journal of Virology [Category] Coronavirus, MERS, [키워드] attenuate attenuation bronchitis cause Cell contagious coronavirus cultures develop Effective vaccines Egg Evolution Ex vivo exhibited Final genomic genomic variation growth High-throughput sequencing highlight IBV in vivo independent Infectious Bronchitis Virus kidney Live attenuated vaccine mechanism molecular mechanism Multiple Mutation nucleocapsid organ pattern phenotype Population raise replicate replicates required Respiratory disease Result sequence sequenced Single nucleotide polymorphism SNP SNPs structural protein tracheal Vaccine Vaccine design Vaccines Variation virulence virulent virus [DOI] 10.1128/JVI.00492-19 PMC 바로가기 [Article Type] Genetic Diversity and Evolution
Analysis of long non-coding RNAs in neonatal piglets at different stages of porcine deltacoronavirus infectionResearch Article Published on 2019-04-112022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] Antiviral strategies Biomarkers cause characterized contagious coronavirus Degradation diarrhea differentially expressed expression data facilitate functional enrichment Glycolysis High throughput sequencing histidine identifying Infection infection process Infectious disease Interaction Interaction network intestinal intestinal wall investigated involved late stage lncRNA long non-coding RNA Long non-coding RNA (lncRNA) metabolism miRNAs mRNA Necrosis Neonatal pathway Porcine deltacoronavirus Porcine deltacoronavirus infection qRT-PCR validation reported Research Result Sequencing significant effect small intestine Stage supplementary material villus viral replication virulence vomiting [DOI] 10.1186/s12917-019-1862-4 PMC 바로가기 [Article Type] Research Article
Mucosal immune responses induced by oral administration recombinant Bacillus subtilis expressing the COE antigen of PEDV in newborn pigletsResearch Articles Published on 2019-02-262022-10-28 Journal: Bioscience Reports [Category] Coronavirus, MERS, [키워드] administration antibody Antigen B. subtilis Bacillus subtilis candidate vaccine cause CD3 CD4 cells COE Contact contagious demonstrated develop disease effective epidemic diarrhea evaluated expressing IgA IgG immune immune response immunization Immunoglobulin immunoglobulin A Infection Local lymphocyte mucosal mucosal immune mucosal immunity Newborn oral administration Oral vaccine PEDV Peyer’s patche Piglets Porcine epidemic diarrhea virus prevented recombinant Bacillus subtilis responses secreting serum SIgA significantly significantly increased Spread systemic immune response T cells up-regulated Vaccine Vaccines virus [DOI] 10.1042/BSR20182028 PMC 바로가기 [Article Type] Research Articles
Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle trackingArticle Published on 2019-02-042022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] addition Analysis cause Cell cell membrane Characteristics contagious cytoplasm disease epidemic diarrhea host cells infection mechanism live cells mechanism microtubule organizing center PEDV performed provided Real-time analysis Transport Vero cells virus [DOI] 10.1038/s41598-018-37789-9 PMC 바로가기 [Article Type] Article
Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in PigletsArticle Published on 2019-01-142022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] Administered administration antibody Antigen cause chain cloned contagious coronavirus death diarrhea effective epidemic diarrhea epitope expression fragment higher affinity IFA Infection inhibit intestinal investigated lesions less mild clinical symptoms Mortality Neonatal neutralizing antibody passive immunization PEDV plaque reduction neutralization Plasmid Porcine epidemic diarrhea virus protection protein expression scFv screened selected shown significantly significantly increased single chain fragment variable (scFv) spike (S) protein Support survival rate targeting the Spike the spike protein Treatment variable viral shedding virus [DOI] 10.3390/v11010058 PMC 바로가기 [Article Type] Article
Porcine epidemic diarrhea virus S1 protein is the critical inducer of apoptosisResearch Published on 2018-11-072022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] accompanied Activation age AIFM1 analyzed Apoptosis Apoptosis-inducing factor mitochondria associated 1 (AIFM1) caspase-3 caspase-8 caspase-9 caused Cell cell membrane characterized cleavage Concentration contagious contribute coronavirus Coronavirus infection Critical cytopathic effects demonstrated diarrhea disease effective epidemic diarrhea expression flow cytometry functional protein high mortality host cell host cells IBV immunofluorescence assay in vitro in vivo induce inducer infect infected cell infected cells inhibit less MERS molecular non-structural protein Nsp1–16 nuclear ORF3 overexpressing overexpression PARP PEDV Plasmid porcine epidemic diarrhea virus (PEDV) Protein Result S1 and S2 S1 protein spike spike S1 protein structural protein supplementary material syncytium formation tested TGEV the S1 protein transfected cells Vero VERO-E6 Vero-E6 cell virus was measured watery wild-type strain [DOI] 10.1186/s12985-018-1078-4 PMC 바로가기 [Article Type] Research
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In VivoVaccines and Antiviral Agents Published on 2018-09-122022-10-30 Journal: Journal of Virology [Category] Coronavirus, MERS, [키워드] avian infectious bronchitis virus BeauR bronchitis cause cellular challenge Characteristics characterized chimeric ciliary Clinical sign Clinical signs conducted conformational contagious coronavirus cross-protection determine expressing glycoprotein Heterologous homologous IBV IMPROVE in vitro in vivo induce Limited Live attenuated vaccines M41 mucosal tissue Partial partial protection PROTECT protective immunity recombinant recombinant vaccine reduction in reductions in replicated Replication Respiratory disease responsible rIBV S1 S1 and S2 S2 subunit serotype serum serum antibody single vaccination spike strain surface protein the S1 subunit tracheal Trial vaccination Vaccine Viral load viral RNA load virulent virus [DOI] 10.1128/JVI.01473-18 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patchesResearch Article Published on 2018-08-162022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] activate adaptive administration Allogeneic antibody Antigen Cell contagious DCs dendritic cell dendritic cells epidemic diarrhea Gut IgA IgG antibody IL-1 IL-4 immune immune response Immunity inactivated Inactivated porcine epidemic diarrhea virus Interaction intestinal intestine investigated Lymphocytes mucosal oral administration PEDV Peyer’s patche Peyer’s patches proliferation reported Result Safe secreted cytokine serum SIgA significantly increased stimulated Vaccine viral disease virus [DOI] 10.1186/s12917-018-1568-z PMC 바로가기 [Article Type] Research Article
A Recombinant Newcastle Disease Virus (NDV) Expressing S Protein of Infectious Bronchitis Virus (IBV) Protects Chickens against IBV and NDVArticle Published on 2018-08-102022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] Antigen bronchitis cause chicken Complete contagious contribute develop disease effective elicited expressing expressing S protein genetic engineering genetics IBV immunization immunoblotting Live attenuated vaccines Mutation neutralizing antibody outcome Protective provided recombinant Recombination reproductive resulting S protein S2 protein Safe SPF the disease the S protein Vaccine vaccine candidate vaccine strain variant virulent virus viruses [DOI] 10.1038/s41598-018-30356-2 PMC 바로가기 [Article Type] Article
Oral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in PigletsArticle Published on 2018-03-012022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Lactobacillus casei vaccine anti-inflammatory response Antibody Response Antigen COE contagious dendritic cell dendritic cell-targeting peptide disease Efficacy elicit ELISA enzyme-linked immunosorbent epidemic diarrhea expressing fused growth histopathological IgA IgG immune response indicated Infection lactobacillus Lactobacillus casei Lymph node lymphocyte mice mucosal immune mucosal immunity Newborn oral Oral vaccine PEDV peptide Porcine epidemic diarrhea virus proliferation promote PROTECT recombinant significant level T-helper cell Th1 phenotype vaccination Vaccine virus was used [DOI] 10.3390/v10030106 PMC 바로가기 [Article Type] Article