Development of a Fast SARS-CoV-2 IgG ELISA, Based on Receptor-Binding Domain, and Its Comparative Evaluation Using Temporally Segregated Samples From RT-PCR Positive IndividualsMicrobiology Published on 2021-01-202022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] anti-SARS-CoV-2 antibody anti-SARS-CoV-2 IgG Antibody Response antibody response against antigens autoantibody Comparative component conditions COVID-19 detect detection development diagnostics domain ELISA evaluated Fever groups HBV HCV IgG antibodies IgG ELISA IgG response IgM individual information lack mapped Neutralizing neutralizing antibody positive positive individual Pregnancy RBD reached Receptor binding domain RT-PCR Sample SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 IgG SARS-CoV-2 IgG antibodies SARS-CoV-2 RT-PCR sensitivity sera specificity spike symptomatic individual Test tested total antibodies vaccine response virus [DOI] 10.3389/fmicb.2020.618097 PMC 바로가기 [Article Type] Microbiology
Performance of two rapid point of care SARS-COV-2 antibody assays against laboratory-based automated chemiluminescent immunoassays for SARS-COV-2 IG-G, IG-M and total antibodiesArticle Published on 2021-01-192022-10-29 Journal: Practical Laboratory Medicine [Category] COVID-19, [키워드] 95% CI Abbott analysed antibodies antibody assay Antibody assays Antigen Architect Assay evaluation automated chemiluminescent immunoassay CLIA Concordance condition Control COVID-19 cross-reactivity dsDNA evaluated IgG IgM IMPROVE information Like NPV PanBio performance POCT point of care point-of-care test point-of-care testing POS positive positive RT-PCR PPV Predictive Prevalence Rapid recruited Result Roche RT-PCR SARS-CoV-2 SARS-CoV-2 antibody sensitivity sera specificity subject total antibody utility Viral hepatitis were assessed were used [DOI] 10.1016/j.plabm.2021.e00201 PMC 바로가기 [Article Type] Article
Development of an Automated Chemiluminescence Assay System for Quantitative Measurement of Multiple Anti-SARS-CoV-2 AntibodiesMicrobiology Published on 2021-01-152022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] 95%CI antibody Antigen automated automated chemiluminescent enzyme immunoassay clinical samples COVID-19 COVID-19 patient development domain elapsed enzyme Epidemiology evaluate evaluated exhibited healthy donors IgG Immunity immunoassay Immunoglobulin independent measurement Multiple multiple antibody measurements negative sample nucleocapsid protein objective parameter positive sample quantitative serological test RBD reagent Result SARS-CoV-2 sensitivity Sensitivity and specificity sera serological test Serological testing specificity Spike protein symptoms onset System tested the disease threshold Validity Variability [DOI] 10.3389/fmicb.2020.628281 PMC 바로가기 [Article Type] Microbiology
Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?Article Published on 2021-01-132022-10-04 Journal: Journal of virological methods [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] Adenovirus anti-nucleoprotein antibody Antibody detection Aspergillus fumigatus Bacterial Concordance confidence interval confirmed case coronavirus COVID-19 cross-reactivity diagnosed ELISA ELISA kit ELISA kits enzyme-linked immunosorbent evaluated Fungal infection healthy controls IgG IgG antibody individual Infection infections Influenza virus Klebsiella pneumoniae majority male Mycobacterium tuberculosis Nucleoprotein antigen objective other respiratory virus Pneumonia Protein protocol reactivity respiratory syncytial virus Result rhinovirus SARS-CoV-2 SARS-CoV-2 nucleoprotein SARS-CoV2 sensitivity sera serum IgG serum sample specificity Staphylococcus Streptococcus pneumoniae subject symptom onset virus was determined [DOI] 10.1016/j.jviromet.2021.114064 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Epitope Mapping on Microarrays Highlights Strong Immune-Response to N Protein RegionArticle Published on 2021-01-112022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] click chemistry Cohort collected common cold coronavirus COVID-19 cross-reactivity diagnostic epitope epitope mapping healthy individuals high-density IgG IgM immunodominant immunoreactivity implementation independent mapping Microarray N protein nucleocapsid peptide peptide microarray peptide microarrays polyprotein positive probe Protein reached Region reported SARS-CoV-2 SARS-CoV-2 spike sensitivity sera serological serological test serum sample specificity Strong symptoms onset tested the N protein [DOI] 10.3390/vaccines9010035 PMC 바로가기 [Article Type] Article
Performance Characteristics of the Vidas SARS-CoV-2 IgM and IgG Serological AssaysVidas SARS-CoV-2 IgM 및 IgG 혈청학적 분석의 성능 특성Immunoassays Published on 2021-01-082022-09-10 Journal: Journal of Clinical Microbiology [Category] Coronavirus, SARS, 진단, 치료기술, [키워드] 95% confidence interval acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 assays automated caused clinical clinical sensitivity clinical specificity coefficients Combined coronavirus COVID-19 COVID-19 pandemic cross-reactivity declined Diagnosis Emergency use authorization epidemiological epidemiological surveillance healthy donor Hospitalized IgG IgG responses IgM and IgG IgM/IgG immunoserology Infection pandemic Patient patients performance positive Precision reached remained respiratory respiratory virus response reverse transcriptase reverse transcriptase PCR RT-PCR SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 IgM SARS-CoV-2 infections SARS-CoV-2 patients SARS-CoV-2 PCR SARS-CoV-2-specific antibodies SARS-CoV-2-specific antibody sensitivity sera Seroconversion Serological assay serological assays serological test Serological testing Serological tests significantly higher specific IgM specificity Spread Surveillance symptom onset test results Variation Vidas virus infection was determined [DOI] 10.1128/JCM.02292-20 PMC 바로가기 [Article Type] Immunoassays
Comparison of Five Serological Assays for the Detection of SARS-CoV-2 AntibodiesArticle Published on 2021-01-062022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acquisition agreement assays association Blood comparable Contact contribute Corona COVID-19 COVID-19 patient detection diagnostic Donor EBV first positive FIVE Immunoglobulin immunologically Patient PCR positive proportion ranged recorded reliability respiratory SARS-CoV-2 SARS-CoV-2-specific antibodies sensitivity sera serological result serology shown Surveillance test comparison virus was recorded [DOI] 10.3390/diagnostics11010078 PMC 바로가기 [Article Type] Article
Neutralizing antibody titres in SARS-CoV-2 infectionsSARS-CoV-2 감염에서 항체 역가 중화Observational Study Published on 2021-01-042022-09-10 Journal: Nature Communications [Category] 임상, 진단, 치료기술, [키워드] antibody Antibody responses Antibody titre assays asymptomatic infection asymptomatic infections asymptomatic patients Chain Reaction Cohort collected decrease Detection limit disease elicit endpoints global public health individuals initial Kinetics Medical research Mild mild or asymptomatic infections neutralization Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody titre neutralizing antibody titres onset of symptom onset of symptoms Patient persist plaque reduction neutralization polymerase chain polymerase chain reaction PRNT remained responses reverse transcription reverse transcription polymerase chain reaction robust RT-PCR RT-PCR detection SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 pandemic sera seropositive Serum neutralizing antibody severe disease severity specific neutralizing antibody tested titre virus [DOI] 10.1038/s41467-020-20247-4 PMC 바로가기 [Article Type] Observational Study
Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray코로나바이러스 항원 마이크로어레이를 이용한 코로나19 회복기 혈액 내 SARS-CoV-2 항체 분석Article Published on 2021-01-042022-09-10 Journal: Nature Communications [Category] 진단, 치료기술, [키워드] addition antibody Antibody Response Antibody responses Antigen antigens array Assay systems Blood blood specimens can be used clinical outcomes collected Combination Complete confirmed COVID-19 case convalescent coronavirus coronavirus strains COVID-19 COVID-19 diagnosis diagnosis of COVID-19 diagnostic Diagnostic markers disease ELISA Epidemiology human coronavirus human respiratory viruses immunologically include Infection MERS MERS-CoV Microarray nucleoprotein pandemic Patient PCR testing performed pharyngeal Prevalence profile Protein Protein array analysis Research respiratory respiratory specimen respiratory viruses risk SARS-CoV SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 pandemic SARS-CoV-2 PCR testing sera serologic specimen Spike protein symptomatic the disease the SARS-CoV-2 two groups viral infection [DOI] 10.1038/s41467-020-20095-2 PMC 바로가기 [Article Type] Article
Matrix metalloproteinase 3 as a valuable marker for patients with COVID-19COVID-19 환자를 위한 중요한 표지자로서의 기질 금속단백분해효소 3Article Published on 2021-01-012022-09-11 Journal: Journal of medical virology [Category] 바이오마커, [키워드] analyzed biochemical center Chinese collected Concentration concentrations Control coronavirus disease Coronavirus disease 2019 Coronavirus pneumonia COVID-19 COVID-19 patient Cytokines detect detection healthy individual IL-1β IL-6 Inflammation Inpatients interleukin marker matrix matrix metalloproteinase Medicine metalloproteinase MMP3 MMP3 concentrations Non-COVID-19 normality Novel coronavirus patients with COVID-19 Pneumonia positive correlation prevention Prevention and control proinflammatory cytokines sera serum Serum level serum levels Severity of disease situation SPSS statistically significant Therapeutic approach therapeutic approaches two group two groups university Wilcoxon rank sum Wilcoxon rank sum test [DOI] 10.1002/jmv.26235 PMC 바로가기 [Article Type] Article