High-throughput screening assays for SARS-CoV-2 drug development: Current status and future directionsSARS-COV-2 약물 개발을위한 고 처리량 스크리닝 분석 : 현재 상태 및 향후 방향Review Published on 2021-10-012022-08-31 Journal: Drug discovery today [Category] SARS, 신약개발, 치료제, [키워드] Accuracy acting acute respiratory syndrome acute respiratory syndrome coronavirus anti-SARS-CoV-2 applied approach approved assays benefit coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 current Cytopathic effect reduction assay data-driven detrimental drug Drug discovery Efficiency evaluate High-throughput screening identify In vitro assay leads life cycle mechanism pandemic Quantitative reduction respiratory SARS-CoV-2 SARS-CoV-2 drug SARS-CoV-2 life cycle target the SARS-CoV-2 true positives [DOI] 10.1016/j.drudis.2021.05.012 PMC 바로가기 [Article Type] Review
High-throughput RNA sequencing of paraformaldehyde-fixed single cellsArticle Published on 2021-09-242022-10-04 Journal: Nature Communications [Category] 유전자 메커니즘, [키워드] Cell cell sorting current detect droplet Express fixation gene expression level Gene expression profiling High-throughput screening Host human betacoronavirus identify inactivation incompatible Infection information methanol OC43 paraformaldehyde pathogen pathogenic pathway permeabilized phenotypic pro-inflammatory Protein RNA RNA sequencing second Single Cell single cells single-cell Single-cell RNA sequencing staining subpopulation subpopulations tissue TMEM119 transcript transcriptomic transcriptomic method Treatment upregulation viral genes viral infection viral reactivation virus [DOI] 10.1038/s41467-021-25871-2 PMC 바로가기 [Article Type] Article
Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral ProductsArticle Published on 2021-09-182022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] antioxidant antioxidants Antiviral compounds approved drug caffeic acid Cell clone Compound compounds construction contagious disease dose-dependent manner encoding epidemic diarrhea exhibited expected expressing expression Frame full-length cDNA genetics growth High-throughput screening identify in vitro Infection inhibit lithocholic acid luciferase activity morphology nanoluciferase natural Neonatal nLuc ORF3 oxygen PEDV platform positive correlation predominant produced product recombinant reduce replaced Replication Reverse genetics system screened Screening significantly therapeutic compound turn viral genome virus wild-type [DOI] 10.3390/v13091866 PMC 바로가기 [Article Type] Article
Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive ReviewReview Published on 2021-09-182022-10-30 Journal: Molecules [Category] Coronavirus, SARS, [키워드] addition Analysis approach biochemical biosensors clinical setting Comprehensive coronavirus disease COVID-19 Diagnosis Diseases extraction High-throughput screening improvement Laboratory medical diagnosis Microfluidics Outbreaks pandemic POC point-of-care Point-of-Care (POC) point-of-care testing (POCT) preparation Prevent review Sample sample preparation separations Spread Treatment variety [DOI] 10.3390/molecules26185666 PMC 바로가기 [Article Type] Review
Nested pool testing strategy for the diagnosis of infectious diseases전염병 진단을위한 중첩 풀 테스트 전략Article Published on 2021-09-132022-09-01 Journal: Scientific Reports [Category] SARS, 진단, [키워드] Assay systems best can be used Definition Diagnosis digital PCR Epidemiology gold gold standard Hierarchical High-throughput screening identify implementation infected individual infected individuals Infectious disease Mathematics and computing pandemic pooling Population screening proceed provide quantification raise RNA RT-qPCR SARS-CoV-2 pandemic shown solution tested the SARS-CoV-2 [DOI] 10.1038/s41598-021-97534-7 PMC 바로가기 [Article Type] Article
Comprehensive Consensus Analysis of SARS-CoV-2 Drug Repurposing CampaignsReview Published on 2021-07-272023-06-16 Journal: Journal of Chemical Information and Modeling [Category] MERS, SARS, [키워드] Chemical libraries COVID-19 docking Drug repurposing High-throughput screening main protease SARS-CoV-2 Spike protein Virtual screening [DOI] 10.1021/acs.jcim.1c00384 PMC 바로가기 [Article Type] Review
Metagenomic analysis of a throat swab sample collected in China on a patient infected with Varicella Zoster VirusArticle Published on 2021-07-062023-05-31 Journal: Scientific Reports [Category] Fulltext, 두창, 수두, 홍역, [키워드] Genome-wide association studies High-throughput screening Next-generation sequencing systemic lupus erythematosus viral infection [DOI] 10.1038/s41598-021-93230-8 PMC 바로가기 [Article Type] Article
Bispecific repurposed medicines targeting the viral and immunological arms of COVID-19Covid-19의 바이러스 및 면역 학적 무기를 대상으로하는 이중 특이 적 용도의 의약품Article Published on 2021-06-242022-08-31 Journal: Scientific Reports [Category] 신약개발, 유전자 메커니즘, 치료제, [키워드] Anti-inflammatory Anti-viral approval Arm benefit candidate Candidates caspase caspase-1 clinical evaluation conserved coronavirus Coronavirus infection Coronavirus replication coronavirus strain coronavirus strains COVID-19 drug Drug discovery Enzyme mechanisms epidermal epidermal growth factor growth factor High-throughput screening Human immunological inhibitor M pro Medicine outbreak Outbreaks Papain-like protease protease Protecting Remdesivir reported required safety evaluation SARS-CoV-2 target targets Tarloxotinib therapeutic agents treat X-ray crystallography [DOI] 10.1038/s41598-021-92416-4 PMC 바로가기 [Article Type] Article
A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entryTMPRSS2 발현을 위한 고처리량 스크린으로 SARS-CoV-2 진입을 제한할 수 있는 FDA 승인 화합물 식별Article Published on 2021-06-232022-09-10 Journal: Nature Communications [Category] MERS, SARS, 비임상, [키워드] 2019-nCoV ACE2 breath Cell cell entry cell surface receptor clinical trial Compound compounds Concentration concentrations coronavirus COVID-19 disease COVID-19 infection DCAF1 DDB1 Degradation dependent on drug E3 ubiquitin ligase Effect entry of SARS-CoV-2 epithelial cells expression global pandemic halofuginone help High-throughput screening homoharringtonine Host host protease host proteases human clinical trial human clinical trials Human plasma identify in vitro library limit lung epithelial cell modulate pathogenic coronavirus pharmacokinetic data proteasome Protein reduce reducing responsible SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 glycoprotein SARS-COV-2 infection Screen Serine serine protease serine protease TMPRSS2 small molecule Small molecules spike stability surface expression surface receptor the cell TMPRSS2 TMPRSS2 expression TMPRSS2 level TMPRSS2 levels TMPRSS2 protein [DOI] 10.1038/s41467-021-24156-y PMC 바로가기 [Article Type] Article
High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA VirusesArticle Published on 2021-06-222022-10-30 Journal: Molecules [Category] COVID-19, SARS, [키워드] 384-well plates acute respiratory syndrome antivirals can be used caused coronavirus COVID-19 pandemic discovery disease drug energy transfer Epidemics example flavivirus fluorescent High-throughput screening inhibitor pandemic papain-like Papain-like protease polio protease Proteases RNA RNA viruses robust SARS-CoV-2 Screening Spanish flu TBEV tick-borne encephalitis virus widespread [DOI] 10.3390/molecules26133792 PMC 바로가기 [Article Type] Article