A Hybrid Soluble gp130/Spike-Nanobody Fusion Protein Simultaneously Blocks Interleukin-6 trans-Signaling and Cellular Infection with SARS-CoV-2하이브리드 가용성 gp130/스파이크-나노바디 융합 단백질은 SARS-CoV-2로 인터루킨-6 트랜스-시그널링 및 세포 감염을 동시에 차단합니다Article Published on 2022-02-232022-09-12 Journal: Journal of Virology [Category] MERS, 진단, [키워드] acute respiratory syndrome addition age anti-inflammatory therapies antibody Antiviral approved assays bind block Cell Clinical use Comorbidities complexes Concentration coronavirus coronavirus disease COVID-19 cytokine receptor Cytokine storm diabetes disease pathology effective fused fusing fusion Glucocorticoid treatment high affinity higher risk hybrid hyperinflammation IL-6 IL-6 receptor IL-6R Immunocompromised patient individual induce infected individuals infected patients Infection inhibitor inhibitors intensive care interleukin-6 life-threatening Lung disease Mild monoclonal antibody monoclonal antibody treatment Multiorgan nanobody pandemic Patient patients Phosphorylation Prevent proliferation Protein RBD Receptor binding domain SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine severe COVID-19 sgp130 shown Signaling sIL-6R soluble IL-6R Spike protein STAT3 Symptoms symptoms of COVID-19 the SARS-CoV-2 the spike protein therapeutic options therapy trans-signaling trans-signaling. treat Vero cells viral cell viral infection virus-induced hyperinflammation [DOI] 10.1128/JVI.01622-21 PMC 바로가기 [Article Type] Article
Allosteric Determinants of the SARS-CoV-2 Spike Protein Binding with Nanobodies: Examining Mechanisms of Mutational Escape and Sensitivity of the Omicron VariantArticle Published on 2022-02-162022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, COVID19(2023년), SARS, [키워드] ACE2 host receptor ACE2-binding site allosteric interactions Alter Analysis binding binding affinity binding epitope binding free energy biochemical Combination complex complexes computational analysis dependent on determinant detrimental escape escape mutant examined Examining functional generate hotspot induce less mechanism modulation molecular dynamics Molecular simulation Mutation mutational sensitivity nanobodies nanobody network analysis neutralization neutralizing capacity omicron performed Perturbation plasticity Regulatory residues Resilience SARS-CoV-2 spike protein signal transmission spike Spike protein spike trimer supported the SARS-CoV-2 the SARS-CoV-2 virus variant [DOI] 10.3390/ijms23042172 PMC 바로가기 [Article Type] Article
An ultrapotent RBD-targeted biparatopic nanobody neutralizes broad SARS-CoV-2 variantsArticle Published on 2022-02-092022-10-04 Journal: Signal Transduction and Targeted Therapy [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] amino acid substitutions antibodies antibody B.1.1.529 B.1.1.7 B.1.351 B.1.617.1 B.1.617.2 B.1.621 Beta binding epitope Clinical use convalescent patient Delta determine domain Drug development Effectiveness experiment extension fusing Gamma highlight host adaptation human IgG1 Fc identify IMPROVE Lambda Microbiology Mutation mutations nanobody neutralization breadth neutralization potency neutralize Neutralizing activity neutralizing antibody omicron P.1 RBD RBD mutations SARS-CoV-2 SARS-CoV-2 neutralization activity SARS-CoV-2 variant spike stability tested the RBD therapeutic Transmission Vaccine variant virus [DOI] 10.1038/s41392-022-00912-4 PMC 바로가기 [Article Type] Article
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesisCDR 스와핑 돌연변이 유발을 사용하여 합성 라이브러리에서 고친화성 나노바디를 쉽게 분리Comment Published on 2022-01-202022-09-11 Journal: STAR protocols [Category] SARS, 변종, [키워드] Affinity maturation antibody approach bioengineering Biotechnology Biotechnology and bioengineering CDR CDRs Cell Biology complementarity-determining region Complete expression Flow flow cytometry Flow Cytometry/Mass Cytometry immunization Isolation Molecular biology Mutagenesis nanobodies nanobody Neutralizing optimization Protein Biochemistry protein expression Protein expression and purification protocol purification SARS-CoV-2 sorting synthetic library variant [DOI] 10.1016/j.xpro.2021.101101 PMC 바로가기 [Article Type] Comment
A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo이중특이성 단량체 나노바디는 스파이크 삼량체 이량체를 유도하고 생체 내에서 SARS-CoV-2를 중화합니다Article Published on 2022-01-102022-09-10 Journal: Nature Communications [Category] SARS, 변종, 진단, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibodies binding Antiviral Beta Beta variant binding coronavirus cross-neutralize Delta variants elicited host cell host cell receptor human ACE2 in vivo inactivation induce inhibit interact Isolation mechanisms mice monomeric nanobody neutralization neutralize RBD RBDs respiratory SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus spike trimer therapeutic Therapeutic molecule therapeutic molecules therapeutic potential Transgenic mouse Trimers variant variants variants of concern Viral Viral antigen viral antigens viral infection virus [DOI] 10.1038/s41467-021-27610-z PMC 바로가기 [Article Type] Article
The role of single-domain antibodies (or nanobodies) in SARS-CoV-2 neutralizationSARS-CoV-2 중화에서 단일 도메인 항체(또는 나노바디)의 역할Review Published on 2022-01-012022-09-12 Journal: Molecular biology reports [Category] MERS, SARS, 진단, [키워드] absence ACE2 acute respiratory syndrome antibodies antibody clinical trials coronavirus COVID-19 COVID-19 pandemic disrupt domain Human infect Interaction nanobodies nanobody neutralization physical produced RBD SARS-CoV-2 SARS-CoV-2 neutralization single-domain antibodies stability the receptor-binding domain therapy Treatment viable treatment [DOI] 10.1007/s11033-021-06819-7 PMC 바로가기 [Article Type] Review
Deep mutational engineering of broadly-neutralizing nanobodies accommodating SARS-CoV-1 and 2 antigenic driftSARS-CoV-1 및 2 항원 드리프트를 수용하는 광범위 중화 나노바디의 심층 돌연변이 공학Article Published on 2022-01-012022-09-11 Journal: mAbs [Category] SARS, 변종, 진단, [키워드] ACE2 amino acids antibody antibody engineering antigenic applied binding contribute cross-reactivity deep deep mutational scanning Efficiency epitope high affinity immunodominant Interaction molecular motif nanobodies nanobody neutralize Neutralizing Pathogens RBD Receptor-binding domain Recognition regions sarbecovirus SARS-CoV-1 SARS-CoV-1 and 2 SARS-CoV-1 RBD SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-CoV-2 RBD screened specificity Substitution variant VHH72 virus yeast yeast surface display. [DOI] 10.1080/19420862.2022.2076775 PMC 바로가기 [Article Type] Article
Nebulized delivery of a broadly neutralizing SARS-CoV-2 RBD-specific nanobody prevents clinical, virological, and pathological disease in a Syrian hamster model of COVID-19광범위하게 중화하는 SARS-CoV-2 RBD 특이적 나노바디의 분무 전달은 COVID-19의 시리아 햄스터 모델에서 임상, 바이러스 및 병리학적 질병을 예방합니다Article Published on 2022-01-012022-09-11 Journal: mAbs [Category] 변종, 진단, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin angiotensin converting enzyme antibody binding site complementary Contact coronavirus COVID-19 disease effective enzyme in vitro in vivo Isolation Lung pathology molecular basis nanobody nebulized nebulized delivery neutralization Neutralizing neutralizing nanobody overlapping pandemic pilot study Prevent pseudoviruses RBD RBD mutations receptor Receptor binding Receptor-binding domain reductions in reported respiratory reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudoviruses SARS-CoV-2 RBD severe acute respiratory syndrome Coronavirus single-domain antibody Support surface area the receptor-binding domain therapeutic Treatment unmet need variant Viral viral burden Virological weight loss [DOI] 10.1080/19420862.2022.2047144 PMC 바로가기 [Article Type] Article
A rapid and affordable point of care test for antibodies against SARS-CoV-2 based on hemagglutination and artificial intelligence interpretationArticle Published on 2021-12-302022-10-04 Journal: Scientific Reports [Category] 변종, 진단, [키워드] acquisition Algorithm Analysis antibody Antigen binding Care Cell clinical study COVID-19 pandemic COVID-19 patient current datasets defined detect diagnostic test Diseases Emergency use authorization Guidance Health Health care instantaneous Interpretation limitation measure Microbiology nanobody Pathogens POC POC testing point of care point-of-care Protein provide RBC RBD red blood cell SARS-CoV-2 sensitivity specificity Support the receptor-binding domain the Spike vaccinated individuals variety while [DOI] 10.1038/s41598-021-04298-1 PMC 바로가기 [Article Type] Article
Biparatopic nanobodies protect mice from lethal challenge with SARS‐CoV‐2 variants of concern2중 파라토프 나노바디는 우려되는 SARS-CoV-2 변종으로 인한 치명적인 공격으로부터 마우스를 보호합니다Article Published on 2021-12-202022-09-10 Journal: EMBO Reports [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (Beta active against Analysis B.1 B.1.351 B.1.617.2 Beta cellular circulating circulating variant conserved COVID‐19 Delta Disease progression dose drug candidates effective epitope epitope mapping Epitopes generate high affinity human ACE‐2 mouse human ACE2 immunology in vitro in vivo increased survival Infection Inflammatory response intranasally lethal dose Lungs mice Microbiology, Virology & Host Pathogen Interaction Molecular Biology of Disease nanobodies nanobody neutralize Neutralizing neutralizing nanobodies pandemic Prevent PROTECT RBD receptor‐binding domain reduced SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 significantly Taking Therapeutics These data transgenic mice treat treated variants of concern variety Viral Viral load VoC VOCs [DOI] 10.15252/embr.202153865 PMC 바로가기 [Article Type] Article