Evaluation on the use of Nanopore sequencing for direct characterization of coronaviruses from respiratory specimens, and a study on emerging missense mutations in partial RdRP gene of SARS-CoV-2호흡기 표본에서 코로나바이러스의 직접적 특성 규명을 위한 나노포어 염기서열 분석의 사용에 대한 평가 및 SARS-CoV-2의 부분 RdRP 유전자에서 출현하는 미스센스 돌연변이에 대한 연구Short Report Published on 2020-11-232022-09-10 Journal: Virology Journal [Category] SARS, 변종, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affecting Amplicon amplicons amplified antiviral drug antiviral drugs catastrophic collected component Consensus conserved coronavirus coronavirus disease Coronavirus disease 2019 Coronaviruses COVID-19 COVID-19 outbreak database Decision making diagnostic domains Efficacy etiologic agent Europe evaluated feasibility Flongle Frequency genetic marker Genome GISAID global healthcare high mutation rate highest highlight Hong Kong human coronaviruses identify knowledge MinION Missense mutation Missense mutations mutation rate mutations Nanopore nanopore sequencing nucleotide pandemic Partial platform positive provided public health RdRP RdRp gene RdRp sequences respiratory respiratory specimens RNA polymerase RNA viruses SARS-CoV-2 sequence sequenced Sequencing severe acute respiratory syndrome Coronavirus Single nucleotide variant SNV SNVs specimen specimens Spread Surveillance Vaccines variant virus [DOI] 10.1186/s12985-020-01454-3 PMC 바로가기 [Article Type] Short Report
Identification of nsp1 gene as the target of SARS-CoV-2 real-time RT-PCR using nanopore whole-genome sequencing나노포어 전체 게놈 시퀀싱을 이용한 SARS-CoV-2 실시간 RT-PCR의 표적인 nsp1 유전자 식별Article Published on 2020-11-012022-09-11 Journal: Journal of medical virology [Category] SARS, 변종, 진단, 치료기술, 치료법, 치료제, [키워드] 95% CI 95% confidence interval acute respiratory syndrome acute respiratory syndrome coronavirus addition Affect binding binding site caused Chain Reaction Clinical severity coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic COVID-19 patient Critical Diagnosis diagnostic diagnostic specificity E gene envelope expressed false-negative false-negative results Frame Genes identification management Molecular assay molecular assays Mutation Nanopore nanopore sequencing nasopharyngeal nonstructural protein 1 nsp1 nsp1. nucleocapsid Open reading frame pandemic Patient Patient management PCR assay PCR assays polymerase chain reaction primer Primers probe probes real-time RT-PCR respiratory RT-PCR RT-PCR assay RT-PCR assays saliva specimen saliva specimens SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 virus SARS-CoV-2 viruses sensitivity sequence severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 single-primer amplification specificity specimen the SARS-CoV-2 genome validation viruses whole-genome sequencing [DOI] 10.1002/jmv.26140 PMC 바로가기 [Article Type] Article
Whole-Genome Sequence of SARS-CoV-2 Isolate Siena-1/2020Genome Sequences Published on 2020-09-242022-10-29 Journal: Microbiology Resource Announcements [Category] COVID-19, [키워드] acute respiratory syndrome Amino acid Amplicon sequencing approaches coding sequence Complete coronavirus D614G Direct RNA sequencing Europe genome sequence isolate Lineage Mutation nanopore sequencing prevalent SARS-CoV-2 the spike protein was obtained [DOI] 10.1128/MRA.00944-20 PMC 바로가기 [Article Type] Genome Sequences
Nanopore sequencing from extraction-free direct PCR of dried serum spots for portable hepatitis B virus drug-resistance typingArticle Published on 2020-08-012023-06-11 Journal: Journal of Clinical Virology [Category] B형 간염, [키워드] dried blood spot genotyping Hepatitis B virus nanopore sequencing Portable diagnostic test Resistance-associated substitutions [DOI] 10.1016/j.jcv.2020.104483
Genome Sequences of SARS-CoV-2 Strains Detected in Hong KongGenome Sequences Published on 2020-07-302022-10-29 Journal: Microbiology Resource Announcements [Category] COVID-19, [키워드] acute respiratory syndrome clinical feature coronavirus coronavirus disease COVID-19 Epidemiology Genome genomic Hong Kong nanopore sequencing Saliva SARS-CoV-2 sequence sequenced strain [DOI] 10.1128/MRA.00697-20 PMC 바로가기 [Article Type] Genome Sequences
Rapid and Sensitive Direct Detection and Identification of Poliovirus from Stool and Environmental Surveillance Samples by Use of Nanopore Sequencing폴리오바이러스의 신속하고 민감한 직접 검출 및 식별: 나노포어 시퀀싱을 이용한 대변 및 환경 감시 샘플에서Article Published on 2020-07-012024-07-28 Journal: Journal of Clinical Microbiology [Category] 폴리오, [키워드] enterovirus Environmental surveillance nanopore sequencing Poliovirus Stool [DOI] 10.1128/JCM.00920-20 PMC 바로가기
Nanopore Targeted Sequencing for the Accurate and Comprehensive Detection of SARS‐CoV‐2 and Other Respiratory VirusesFull Paper Published on 2020-06-242023-06-15 Journal: Small (Weinheim an der Bergstrasse, Germany) [Category] MERS, SARS, [키워드] COVID‐19 nanopore sequencing respiratory virus SARS‐CoV‐2 targeted amplification [DOI] 10.1002/smll.202002169 PMC 바로가기 [Article Type] Full Paper
Influenza A Virus Field Surveillance at a Swine-Human InterfaceArticle Published on 2020-02-052023-06-23 Journal: mSphere [Category] 신종인플루엔자, [키워드] Influenza mobile sequencing nanopore sequencing pandemic preparedness Swine influenza [DOI] 10.1128/mSphere.00822-19 PMC 바로가기