Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2Article Published on 2022-02-072022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] acute respiratory syndrome approach bioprocess cGMP Characteristics characterization co-expression coronavirus coronavirus spike protein COVID-19 design of experiments different disease DoE downstream Downstream process ENhance experiment expression Gag generate genetic material good manufacturing practice HEK293 HIV-1 immunogenic membrane protein methodology optimization Particle performed platform Production Protein purification robust Safe SARS-CoV-2 SARS-CoV-2 VLP Structure transient transfection vaccine candidate Vaccine development variants virus VLP VLP vaccines VLPs wild-type [DOI] 10.3390/vaccines10020250 PMC 바로가기 [Article Type] Article
Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCRSARS-CoV-2 양성 대상자의 사후 폐 샘플에서 다양한 수준의 스파이크 및 ORF1ab RNA: ISH와 RT-PCR 간의 비교Original Article Published on 2022-02-012022-09-10 Journal: Virchows Archiv [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] amplification amplification cycle amplification cycles analyzed cellular Chain Reaction correlation Deceased demonstrated detect E gene electron microscopy envelope FFPE formalin formalin-fixed paraffin-embedded Frame gene amplification IHC immunohistochemistry In situ hybridization In situ hybridization (ISH) Microscopy morphology N gene nucleocapsid Open reading frame Open reading frame (ORF1ab) optimization ORF1 ORF1ab ORF1ab gene paraffin polymerase chain polymerase chain reaction positive Post-mortem post-mortem lung Post-mortem lung samples reverse transcription reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) RNA RT-PCR S gene SARS-CoV-2 sensitivity sequence Spike(S) staining subjects supplementary material the SARS-CoV-2 tissue variable Viral Viral RNA virus virus detection [DOI] 10.1007/s00428-021-03262-8 PMC 바로가기 [Article Type] Original Article
The impact of COVID-19 on cancer care and oncology clinical research: an experts’ perspectiveCOVID-19가 암 치료 및 종양학 임상 연구에 미치는 영향: 전문가의 관점Review Published on 2022-02-012022-09-11 Journal: ESMO open [Category] COVID19(2023년), MERS, SARS, 임상, 진단, [키워드] accelerate addition approach approaches Area article assessment Cancer cancer care Care catalyst Clinical research clinical trial clinical trials collaborative framework collected coronavirus coronavirus disease Coronavirus disease-19 COVID-19 COVID-19 pandemic described driving Efficacy endpoints Evidence faster Generalizability group Impact implementation IMPROVE include International IQVIA lead limit Local minority groups oncology optimization outcome pandemic Patient Perspective positive Real-world evidence Research risk Support Telemedicine timeline timelines Topic Treatment Trial [DOI] 10.1016/j.esmoop.2021.100339 PMC 바로가기 [Article Type] Review
Automated liquid handling robot for rapid lateral flow assay development신속한 측면 유동 분석 개발을 위한 자동화된 액체 처리 로봇Research Paper Published on 2022-01-292022-10-28 Journal: Analytical and bioanalytical chemistry [Category] COVID-19, MERS, SARS, 말라리아, [키워드] Abstract antibody automated Concentration continuous variable demonstrated diagnostics effort experiment handling High-throughput screening Infectious disease Intensive lateral flow lateral flow assay malaria Mycobacterium tuberculosis optimization platform point-of-care Pregnancy reduce reproducibility required resource SARS-CoV-2 supplementary material toxin Validity while [DOI] 10.1007/s00216-022-03897-9 PMC 바로가기 [Article Type] Research Paper
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesisCDR 스와핑 돌연변이 유발을 사용하여 합성 라이브러리에서 고친화성 나노바디를 쉽게 분리Comment Published on 2022-01-202022-09-11 Journal: STAR protocols [Category] SARS, 변종, [키워드] Affinity maturation antibody approach bioengineering Biotechnology Biotechnology and bioengineering CDR CDRs Cell Biology complementarity-determining region Complete expression Flow flow cytometry Flow Cytometry/Mass Cytometry immunization Isolation Molecular biology Mutagenesis nanobodies nanobody Neutralizing optimization Protein Biochemistry protein expression Protein expression and purification protocol purification SARS-CoV-2 sorting synthetic library variant [DOI] 10.1016/j.xpro.2021.101101 PMC 바로가기 [Article Type] Comment
Vaccination Schedule under Conditions of Limited Vaccine Production RateArticle Published on 2022-01-132022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] approved caused Community condition COVID-19 pandemic COVID-19 vaccination Decision-making distribution experiment group herd immune Herd immunity threshold Immunity include Infection International Limited mathematical model optimization pandemic Probability Production Protective Rate reported schedule scheduling stratified the vaccine vaccination vaccination schedule Vaccine Vaccines [DOI] 10.3390/vaccines10010116 PMC 바로가기 [Article Type] Article
Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum AdjuvantsImmunology Published on 2022-01-122022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] acute respiratory syndrome adjuvant adjuvants Administered alum alum adjuvant antigen-specific antibody approach cartilage coronavirus coronavirus disease COVID-19 COVID-19 pandemic effective Effectiveness efficient Euglena evaluated examined expression expression vector highlight host cells induce induction Infection inoculation mice motif mouse serum neutralizing antibody optimization paramylon produced Protein purified S protein SARS-CoV-2 secreted serum Silkworm-baculovirus expression vector system spike spike (S) protein Spread subunit the S protein therapeutic agents trimeric Vaccine Vaccine development was used [DOI] 10.3389/fimmu.2021.803647 PMC 바로가기 [Article Type] Immunology
Optimization of Single-Dose VSV-Based COVID-19 Vaccination in Hamsters햄스터에서 단일 용량 VSV 기반 COVID-19 백신 접종의 최적화Immunology Published on 2022-01-062022-09-11 Journal: Frontiers in Immunology [Category] 변종, 진단, [키워드] CD4 CD8 Clinical sign Clinical signs Combination COVID-19 COVID-19 pandemic Ebola virus Effect Efficacy glycoprotein greater hamster hamsters Health highlight Highlights IgG response immunogenicity intramuscularly lung Lungs NK cell optimization Pneumonia Protective raise rVSV-ZEBOV GP SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variants SARS2 severe acute respiratory syndrome coronavirus-2 Spike protein stability stomatitis T cell T cell response the SARS-CoV-2 vaccination Vaccine vaccine candidate Vaccines vesicular stomatitis vesicular stomatitis virus Viral viral variant Viral variants VSV-EBOV VSV-SARS2 [DOI] 10.3389/fimmu.2021.788235 PMC 바로가기 [Article Type] Immunology
Multi-COVID-Net: Multi-objective optimized network for COVID-19 diagnosis from chest X-ray imagesResearch article Published on 2022-01-012022-10-05 Journal: Applied Soft Computing [Category] 변종, [키워드] automated automatically Care Chest radiography chest X-ray Chest X-ray image Chest X-ray images CNN COVID-19 COVID-19 diagnosis COVID-19 testing CXR datasets deep deep learning efficient evaluate Grasshopper growth Health healthcare service Hospitalized imperative individual individuals information limit MOGOA Multi-objective optimization occurrence of COVID-19 optimization Patient Pneumonia provide researcher Spread stages tested the disease to define Treatment [DOI] 10.1016/j.asoc.2021.108250 [Article Type] Research article
Immuno-Endocrinology of COVID-19: The Key Role of Sex HormonesCOVID-19의 면역 내분비학: 성 호르몬의 주요 역할Review Published on 2021-12-022022-09-11 Journal: Frontiers in Endocrinology [Category] SARS, 유전자 메커니즘, [키워드] Adaptive immune response androgen Clinical studies clinical study coronavirus coronavirus 2019 disease COVID-19 COVID-19 pathogenesis COVID-19 severity Cytopathic effect disease severity element Estrogen Evidence Factor Factors fatality Gender Gene regulation hormone hyper-inflammatory response identify immune response immune responses immuno-endocrinology include Infection Injury involved mechanism optimization Pathogenesis personalised regulated Regulation role SARS-CoV2 severity Sex sex hormone Sex hormones susceptibility target targets therapeutic intervention tissue injury tissue specific Treatment outcome Trial [DOI] 10.3389/fendo.2021.726696 PMC 바로가기 [Article Type] Review