Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy전자 현미경으로 인간 조직의 세포 내 구조에서 코로나바이러스를 구별하는 데 어려움Review Published on 2021-04-012022-09-10 Journal: Emerging Infectious Diseases [Category] MERS, SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition caused characteristic coated coronavirus coronavirus disease COVID-19 COVID-19 patient cross damage difficulty electron electron microscopy Endoplasmic reticulum Extracellular focus Human investigator investigators less membrane-bound morphologic feature morphologic features multivesicular bodies negative stain nucleocapsid pandemic Particles pathology reported respiratory respiratory infections SARS SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike Structure subcellular structures thin sections tissue damage tissues transmission electron microscopy ultrastructure vesicles virus viruses zoonoses [DOI] 10.3201/eid2704.204337 PMC 바로가기 [Article Type] Review
Early postmortem mapping of SARS-CoV-2 RNA in patients with COVID-19 and the correlation with tissue damageCOVID-19 환자의 SARS-CoV-2 RNA의 사후 사후 매핑 및 조직 손상과의 상관 관계Research Article Published on 2021-03-302022-09-10 Journal: eLife [Category] SARS, 진단, 치료기술, 치료제, [키워드] accumulate addition Autopsies Autopsy Blood blood clotting bloodstream cause caused circulating clinical clinical observations coronavirus correlation COVID-19 Deceased detect died disease dissemination distribution drug treatment drug treatments early post-mortem interval effective example experiment extrapulmonary tissue feature help histology host response human body Hypothesis immune reaction impacted Infection Inflammation involved leads lung tissue Lungs more difficult Multiorgan dysfunction New coronavirus observation organ organs other organ other organs pathophysiology Patient patients with COVID-19 performed postmortem examination postmortem examinations postmortem interval Protein provide question regions remained Research SACs sampling SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA Support systemic disease tissue tissue damage tissue degradation tissues transmission electron microscopy Travel Viral Viral load viral particle Viral RNA Viremia virus were used while [DOI] 10.7554/eLife.60361 PMC 바로가기 [Article Type] Research Article
A Novel Computational Approach for the Discovery of Drug Delivery System Candidates for COVID-19COVID-19에 대한 약물 전달 시스템 후보 발견을 위한 새로운 전산 접근 방식Article Published on 2021-03-102022-09-10 Journal: International Journal of Molecular Sciences [Category] 신약개발, [키워드] Algorithm approach CADD candidate Clustering computer-aided drug discovery condition coronavirus disease Coronavirus disease 2019 COVID-19 delivery docking drug drug delivery system Efficacy evaluated functional functional group glycyrrhizin IMPROVE in silico information machine learning micelle nanoparticles molecular molecules morphology nafamostat Nafamostat mesilate novel predicted Schrodinger stability System TEM transmission electron microscopy treat unsupervised learning was selected zeta potential [DOI] 10.3390/ijms22062815 PMC 바로가기 [Article Type] Article
Evaluation of the Antiviral Activity of Sitagliptin-Glatiramer Acetate Nano-Conjugates against SARS-CoV-2 VirusArticle Published on 2021-02-242022-10-28 Journal: Pharmaceuticals [Category] COVID-19, [키워드] 1:1 3CL 3CL Protease Acetate activity acute respiratory syndrome addition Antiviral binding affinity carried cause cellular characterization China Clinical efficacy clinical study complex component Concentration coronavirus Coronavirus replication COVID COVID-19 COVID-19 pandemic criteria docking Efficacy epithelial cell evaluate factorial design Factors FDA-approved drugs Glatiramer acetate half-maximal inhibitory concentration Health IC50 investigated Nanoparticles outbreak Prevent protease protease inhibition required responses SARS-CoV-2 selected shown sitagliptin target cell TEM the SARS-CoV-2 virus transmission electron microscopy treat VERO-E6 virus were assessed [DOI] 10.3390/ph14030178 PMC 바로가기 [Article Type] Article
Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cellsOriginal Article Published on 2021-02-082022-10-29 Journal: Memórias do Instituto Oswaldo Cruz [Category] COVID-19, [키워드] acute respiratory syndrome Antiviral antiviral drug Asia biology of SARS-CoV-2 Cell cell membrane China coronavirus COVID-19 COVID-19 outbreak CoVs Cytopathic effect cytoplasmic demonstrated elevated Endocytic pathway Endoplasmic reticulum in vitro infected cell Infection International maturation membrane MERS-CoV Middle East morphogenesis morphology New coronavirus new virus nucleocapsid occur occurred outbreak pandemic pathway patients polymerase chain Prophylactic public health RER Respiratory disease reverse transcription RT-PCR SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 particle severe respiratory infection Spread susceptibility therapeutic transmission electron microscopy Ultrastructural analysis Vaccine vaccine candidate Vero-E6 cell Vero-E6 cells vesicles viral envelope viral particle virus WHO World Health Organization Wuhan, China [DOI] 10.1590/0074-02760200443 PMC 바로가기 [Article Type] Original Article
Hunting coronavirus by transmission electron microscopy – a guide to SARS-CoV-2-associated ultrastructural pathology in COVID-19 tissues투과전자현미경으로 코로나바이러스 사냥 - COVID-19 조직에서 SARS-CoV-2 관련 미세구조 병리학 가이드Review Published on 2021-02-012022-09-11 Journal: Histopathology [Category] SARS, 치료법, [키워드] Affect anticipated Autopsy change characteristic coated complex coronavirus Coronavirus replication COVID-19 COVID-19 patient COVID-19 patients described electron electron microscopy electron-dense feature hunting induce infected cell culture membrane Microscopy more difficult morphological finding morphological findings multivesicular bodies multivesicular body nucleocapsid organ pathology required respiratory respiratory tract SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication subcellular structures tissue tissues transmission electron microscopy ultrastructure Vesicle Viral viral particle Viral particles viral replication virion virions Virology virus replication Virus-like particle Visualisation [DOI] 10.1111/his.14264 PMC 바로가기 [Article Type] Review
Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein강성 단일클론 항체는 SARS-CoV-2 뉴클레오캡시드 단백질의 검출을 개선합니다Article Published on 2021-01-012022-09-11 Journal: mAbs [Category] 진단, 치료기술, 치료법, [키워드] Affect antibody Antigen assembly biological phenomena complex conducted Critical Detection sensitivity diagnostics early diagnosis enzyme-linked immunosorbent enzyme-linked immunosorbent assay epidemiological studies Epidemiological study epitope Epitopes facilitate flexibility flexible IMPROVE juxtaposition lateral flow linear mAb mAbs mAbs polymerization monoclonal antibodies monoclonal antibody N-terminal domain NTD nucleocapsid nucleocapsid protein pathogen Pathogens point-of-care prevented Protein SARS-CoV-2 SARS-CoV-2 nucleocapsid scattering selective small-angle x-ray scattering. transmission electron microscopy Treatment X-ray scattering [DOI] 10.1080/19420862.2021.1905978 PMC 바로가기 [Article Type] Article
Evidence of SARS-CoV-2 Transcriptional Activity in Cardiomyocytes of COVID-19 Patients without Clinical Signs of Cardiac InvolvementArticle Published on 2020-12-182022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] absence activity addition affected alteration analysed assays asymptomatic patients Autopsy autopsy analysis cardiac Cardiomyocytes cell death clinical Clinical sign collected coronavirus COVID-19 COVID-19 patient death Deceased develop digital PCR disease displaying evidence of heart heart tissue immunofluorescence immunohistochemistry Injury investigated Involvement oedema Patient phenotype proportion Respiratory disease Respiratory failure RNA SARS-CoV-2 SARS-CoV-2 spike SIGN subcellular Surveillance transmission electron microscopy viral transcription [DOI] 10.3390/biomedicines8120626 PMC 바로가기 [Article Type] Article
SARS-CoV-2 spike protein binds to bacterial lipopolysaccharide and boosts proinflammatory activityArticles Published on 2020-12-092022-10-30 Journal: Journal of Molecular Cell Biology [Category] COVID-19, MERS, SARS, [키워드] Activation aggregation Analysis Bacterial bacterial lipopolysaccharide bind Blood boost Cell Comorbidity COVID-19 cytokine response demonstrated dynamic excessive inflammation gel electrophoresis in vitro in vivo infection with SARS-CoV-2 Inflammation Inflammatory response Interaction lipopolysaccharide LPS metabolic syndrome mice modulated molecular Molecular dynamics simulation mononuclear cells Native NF-κB nuclear Patient Peripheral blood proinflammatory Protein reporter S protein SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 spike SARS-CoV-2 spike protein scattering severe COVID-19 Spike protein syndrome THP-1 transmission electron microscopy [DOI] 10.1093/jmcb/mjaa067 PMC 바로가기 [Article Type] Articles
Taishan Pinus massoniana pollen polysaccharide inhibits H9N2 subtype influenza virus infection both in vitro and in vivoArticle Published on 2020-09-012023-06-22 Journal: Veterinary microbiology [Category] 조류인플루엔자, [키워드] Anti-influenza Avian influenza virus H9N2 Pinus massonianapollen polysaccharides transmission electron microscopy [DOI] 10.1016/j.vetmic.2020.108803