Emergence of a Novel Coronavirus (COVID-19): Protocol for Extending Surveillance Used by the Royal College of General Practitioners Research and Surveillance Centre and Public Health EnglandProtocol Published on 2020-04-022022-10-31 Journal: JMIR Public Health and Surveillance [Category] COVID-19, SARS, [키워드] all age groups analyzed approach Asymptomatic Blood blood sample coding Collecting college Community computerized convalescent serum coronavirus Coronavirus infection COVID-19 COVID-19 infection data extraction Effectiveness emergence England FIVE General practice general practitioner geographical distribution Identifier Infection infections Infectious disease Influenza International introduced involved Lower respiratory tract medical record medical record systems MONITOR National novel objective outbreak pandemic Pandemics pathway Patient PHE Population presenting primary care protocol Public Health England Rapid RCGP recording records as topic report reported respiratory infections Result RSC SARS-CoV-2 sentinel surveillance sera serological surveillance serology Spread Support Surveillance Virological [DOI] 10.2196/18606 PMC 바로가기 [Article Type] Protocol
Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoVArticle Published on 2020-03-272022-10-04 Journal: Nature Communications [Category] MERS, SARS, 변종, [키워드] antibodies antibody beta coronavirus biology of SARS-CoV-2 cathepsin L caused Cell characterization CoV COVID-19 Critical cross-neutralization cross-neutralization activity cross-reactivity drug entry pathway enzyme hACE2 immune Infection inhibit less MERS-CoV in 2012 Outbreaks PROTECT pseudovirions pseudovirus receptor SARS virus SARS-CoV SARS-CoV S SARS-CoV-2 SARS-CoV-2 S SARS-CoV-2 S protein sera spike glycoprotein Spike protein Spread T62 target the SARS-CoV-2 Vaccine viral infection virus entry Virus-host interactions zoonotic [DOI] 10.1038/s41467-020-15562-9 PMC 바로가기 [Article Type] Article
Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2Article Published on 2020-03-242022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] Analysis biosafety Biosafety Level 3 Cell cell types Coefficient of variation conditions convalescent patient COVID-19 cutoff value effective vaccine lack limit of detection live SARS-CoV-2 mouse serum Neutralization assay Neutralizing neutralizing antibody parameter pathogenicity protocol pseudovirus Pseudovirus neutralization assay pseudovirus production robust SARS-CoV-2 sera tested the SARS-CoV-2 Vaccine Virological virus virus inoculum viruses VSV was determined [DOI] 10.1080/22221751.2020.1743767 PMC 바로가기 [Article Type] Article
A pneumonia outbreak associated with a new coronavirus of probable bat originArticle Published on 2020-02-032022-10-30 Journal: Nature [Category] COVID-19, MERS, SARS, [키워드] 2019-nCoV acute respiratory syndrome addition Analysis bat Bronchoalveolar lavage fluid caused cell entry characterization conserved coronavirus Critically ill death domain early stage enzyme II Epidemic FIVE full-length genome sequence genome sequence Host Human indicate infect Infection laboratory-confirmed neutralized New coronavirus non-structural protein outbreak Pathogens Patient patients Pneumonia previous study protein sequence SARS-CoV SARS-CoV-2 SARS-related coronavirus SARSr-CoV SARSr-CoVs sequence sequence identity sera Seven shown Virology virus whole-genome Wuhan, China [DOI] 10.1038/s41586-020-2012-7 PMC 바로가기 [Article Type] Article
Middle East Respiratory Syndrome Coronavirus Antibodies in Bactrian and Hybrid Camels from DubaiObservation Published on 2020-01-222022-10-30 Journal: mSphere [Category] Coronavirus, MERS, [키워드] 95% confidence interval acute respiratory syndrome addition affected antibodies antibody Antibody test Bactrian camel Camel China coronavirus correlation Dubai enzyme-linked immunosorbent etiological agent examined Fatality rate greater hybrid hybrid camel Infection intranasally MERS MERS coronavirus MERS-CoV MERS-CoV infection Middle East Mortality neutralization neutralizing antibody titers positive respiratory sera Seroconversion SEROLOGICAL STUDY serum sample Spearman coefficient syndrome tested United Arab Emirate virus Xinjiang [DOI] 10.1128/mSphere.00898-19 PMC 바로가기 [Article Type] Observation
Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine CoronavirusArticle Published on 2019-11-302022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Accuracy Analysis characteristic convalescent sera coronavirus cut-off value demonstrated detection determine development diagnostic ELISA enteric disease enzyme-linked immunosorbent Equine coronavirus Europe France Infection involved Japan outbreak performed Population populations positive reported ROC S1 protein sera Seroconversion Seroprevalence serum sample Specific specificity spike S1 protein Support USA validation Virus neutralization [DOI] 10.3390/v11121109 PMC 바로가기 [Article Type] Article
Development and comparison of enzyme-linked immunosorbent assays based on recombinant trimeric full-length and truncated spike proteins for detecting antibodies against porcine epidemic diarrhea virusResearch Article Published on 2019-11-272022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antibody caused Cell cell line development disease Efficacy ELISA ELISA kit ELISAs embryonic kidney enzyme-linked immunosorbent assay epidemic diarrhea exhibited expressed expression full-length full-length S Genotype high mortality Human embryonic kidney (HEK)-293 mammalian cell expression system humoral hyperimmune immunization immunostaining Impact indirect ELISA infected cell mucosal immune Neonatal nucleocapsid outbreak PEDV Porcine epidemic diarrhea (PED) Protein Result sensitivity Sensitivity and specificity sera Serological assay serological test serology specificity Spike protein Spike protein specific ELISA trimeric virus was selected [DOI] 10.1186/s12917-019-2171-7 PMC 바로가기 [Article Type] Research Article
Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigsResearch Article Published on 2019-11-122022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] (PEDV) Accuracy addition Analysis antibody Antigen applied Asymptomatic AUC characteristic coated contagious Culture cut-off value develop development Diagnostic method diarrhea disease Efficacy ELISA ELISA plate ELISA test enzyme-linked immunosorbent epidemic diarrhea evaluate evaluated high mortality high mortality rate IgG Infection Infectious disease Japan Long-term preservation neutralization neutralization test Newborn occur occurred outbreak PEDV Porcine epidemic diarrhea virus positive positive correlation Pre-coated plate preserved reference method Result ROC Sensitivity and specificity sera serological survey serum serum samples specific antibody symptomatic Vero Cell virus vomiting was determined watery [DOI] 10.1186/s12917-019-2123-2 PMC 바로가기 [Article Type] Research Article
Serological evidence of MERS-CoV and HKU8-related CoV co-infection in Kenyan camelsArticle Published on 2019-10-242022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, MERS, [키워드] antibody bat Camel circulation Co-infection coronavirus CoV CoV genome CoVs domain Dromedary Evidence HKU8 Host human infections Infection investigator Kenya MERS MERS-CoV MERS-CoV RBD Middle East molecular nucleocapsid positive proportion raise RBD recombinants Recombination respiratory syndrome coronavirus sera seropositive Spike protein undergo various coronaviruses virus [DOI] 10.1080/22221751.2019.1679610 PMC 바로가기 [Article Type] Article
Serological Screening for Coronavirus Infections in CatsArticle Published on 2019-08-132022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] addition Amino acid analyzed antibody antibody epitope antigenic Antisera cats Cell cell entry conserved coronavirus Coronavirus infection Coronaviruses CoV CoVs cross-reaction cross-reactivity Cross-species transmission diagnoses domain ELISA engage epidemic diarrhea Evidence excluded HCoV-229E human coronavirus 229E immunogenic indicated infect Infection mammal ortholog PEDV positive presence of antibody receptor Receptor binding reported S1 protein screened Screening sequence identity sera serological serum serum sample Spike protein subunit supported virus Virus neutralization widespread [DOI] 10.3390/v11080743 PMC 바로가기 [Article Type] Article