Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirusArticle Published on 2018-04-042022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, [키워드] addition anti-S antigen expression clinical trials control group effective ELISpot assay enhanced Final highlight IgG response immune response immunization immunized inactivated Infection interferon-γ Live virus lung membrane MERS-CoV mice Middle East Neutralizing activity nucleocapsid plaque-forming units protective immunity Protein Proteins public health threat reduced respiratory syndrome coronavirus Safe sera spike Spike protein structural proteins T-cell immunity tested the S protein transduced vaccination Vaccine Vaccines virus virus titers [DOI] 10.1038/s41426-018-0056-7 PMC 바로가기 [Article Type] Article
Development of a one-step RT-PCR assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) using newly designed degenerate primers for porcine and avian `fecal samplesArticle Published on 2018-02-272022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] amplify Analysis Asia bat biological sample biological samples characterized conditions conserved Coronaviruses CoV CoVs Critical degenerate Degenerate primers demonstrated detect develop development domain ENCODE enteric diseases epidemic diarrhea Epidemics fecal gastroenteritis intestinal Laboratory measure Pancoronavirus RT-PCR pathogen PEDV Porcine deltacoronavirus positive primer Protein reported RNA RNA-dependent RNA polymerase RNA-dependent RNA polymerase (RdRP) gene RT-PCR RT-PCR assay RT-PCR assays Sensitivity and specificity sequenced sequenced samples sera serum samples tested universal primers virus [DOI] 10.1016/j.jviromet.2018.02.021 PMC 바로가기 [Article Type] Article
Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virusResearch Published on 2018-02-092022-10-29 Journal: Microbial Cell Factories [Category] Coronavirus, MERS, [키워드] Administered administration antibody Antigen caused Cell cells Cellular immune response COE cytokine DCs dendritic dendritic cell dendritic cell-targeting peptide epidemic diarrhea epitope evaluated expressed expressing fused genitalis group humoral IL-4 immunization immunized in vitro induce Infection intestinal lactobacillus Lactobacillus casei Ligand ligands mice Microfold cell-targeting peptide mucosal Neutralizing Neutralizing activity oral Oral Immunization PEDV peptide Porcine epidemic diarrhea virus proliferation Result sera significant difference significantly higher specific IgG antibody Spike protein Stool strain supplementary material tractus Vaccine virus [DOI] 10.1186/s12934-018-0861-7 PMC 바로가기 [Article Type] Research
Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISAArticle Published on 2017-10-062022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] antibody Antibody detection Antibody Response characterization characterized competitive ELISA conducted correlated correlation detect develop Diagnosis ELISA epidemiological Epidemiology Epitopes Ethiopian gold standard immunized Live virus MERS MERS coronavirus MERS-CoV MERS-CoV infection Middle East monoclonal antibody neutralization neutralizing antibody novel Patient performed produced relative respiratory syndrome coronavirus Sensitivity and specificity sera Serological testing serum antibody serum dilution severe respiratory illness Spike protein the spike protein virus virus neutralization test was used zoonotic [DOI] 10.1016/j.jviromet.2017.10.008 PMC 바로가기 [Article Type] Article
Cross-sectional surveillance of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016Surveillance and Outbreak Report Published on 2017-03-162022-10-31 Journal: Eurosurveillance [Category] Coronavirus, Fulltext, MERS, 조류인플루엔자, [키워드] addition age antibody bat bats Camel Camel, Ruminants clade Cluster collected conducted Contact cross-sectional determine East Egypt Equines genetic material mammal MERS-CoV Middle East nasal swabs Neutralising Antibodies neutralising antibody positive Prevalence Rectal swabs respiratory syndrome coronavirus ruminant sampling sequence sera seropositive Seroprevalence significantly higher Surveillance tested titre Transmission virus [DOI] 10.2807/1560-7917.ES.2017.22.11.30487 PMC 바로가기 [Article Type] Surveillance and Outbreak Report
Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in ChinaOriginal Article Published on 2016-11-092022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, [키워드] antibody Antibody titer Antigen characterization China correlated correlation domain ELISA ELISAs enzyme-linked immunosorbent enzyme-linked immunosorbent assay Extracellular inactivated Infection MERS MERS coronavirus MERS-CoV Middle East N-terminal domain neutralization neutralizing antibody NTD occurred Patient pseudovirus RBD RBD ELISA recombinant structural antigens sera Seroconversion significantly Strong syndrome the patient [DOI] 10.1038/emi.2016.114 PMC 바로가기 [Article Type] Original Article
Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigsResearch Article Published on 2016-06-102022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antibody antibody kinetics Antibody Response approach collected Cutoff declined diagnostic ELISA epidemic diarrhea estimate fecal samples Final fluids IgA IgA response IgG Longitudinal sample nucleic acid oral fluids PEDV populations positive provided rectal rectal swab Rectal swabs Result rRT-PCR S/P sensitivity sera serum serum IgG serum sample Site specificity Surveillance Swab virus Virus shedding were used [DOI] 10.1186/s12917-016-0725-5 PMC 바로가기 [Article Type] Research Article
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) origin and animal reservoirReview Published on 2016-06-032022-10-28 Journal: Virology Journal [Category] Coronavirus, Fulltext, [키워드] acute respiratory syndrome animal Animal host bat bats Camels coronavirus disease Dromedary East Endemic geographical distribution human infections humans introduced investigated involved MERS-CoV Middle East Middle East respiratory syndrome Novel coronavirus Phylogenetic Population reservoir respiratory responsible sera suggested Surveillance virus [DOI] 10.1186/s12985-016-0544-0 PMC 바로가기 [Article Type] Review
Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23Article Published on 2016-05-072022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] Analysis Antibody assays antibody tests Antibody titer Camel cells characterization Cluster codon coronavirus correlation CoV Cytopathic effect Dromedary Dromedary camel Fecal sample FIVE Genetic heat-inactivated immunofluorescence Isolation MERS MERS-CoV mice middle Middle East motif N gene N protein neutralization Neutralization assay Northern blot analysis Pearson predicted RdRP reported respiratory syndrome coronavirus RNA separated sequence sera Seven strain subgenomic mRNA transcription regulatory sequence [DOI] 10.3390/ijms17050691 PMC 바로가기 [Article Type] Article
Trigger factor assisted soluble expression of recombinant spike protein of porcine epidemic diarrhea virus in Escherichia coliResearch Article Published on 2016-05-042022-10-27 Journal: BMC Biotechnology [Category] Coronavirus, [키워드] antigens chaperone Chaperone co-expression system cloned co-expression condition contagious develop elicited ELISA epidemic diarrhea Escherichia coli expressed expression expression vectors immune immunization immunogenic Inclusion inclusion bodies mAb mice neutralizing antibody pathogen PEDV produced Protein protein antigen Proteins purified reactivity Recombinant protein recombinant proteins Recombinant spike protein Result S protein sera serum IgG Serum neutralizing antibody spike glycoprotein Strains subunit Subunit vaccine supplementary material tested Trigger Trigger factor Vaccine variety virus western blot [DOI] 10.1186/s12896-016-0268-7 PMC 바로가기 [Article Type] Research Article