Crystal Structure of the C-Terminal Cytoplasmic Domain of Non-Structural Protein 4 from Mouse Hepatitis Virus A59Research Article Published on 2009-07-102022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Amino acid asymmetric unit C-terminal complex conformation conserved coronavirus Coronavirus replication crystal crystal structure cytoplasmic cytosol determined different dissociate disulfide bond domain Endoplasmic reticulum ER membrane expressed feature finding functional hydrophilic infected cells membrane membrane vesicle MHV monomer mouse mutant N- and C-termini non-structural proteins not affect Nsp3 nsp4 opposite physiological recruited reducing Region Replication Ser shown Structure wild-type [DOI] 10.1371/journal.pone.0006217 PMC 바로가기 [Article Type] Research Article
Genome-Wide Analysis of Protein-Protein Interactions and Involvement of Viral Proteins in SARS-CoV ReplicationResearch Article Published on 2008-10-012022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] adopted assays biochemical complex coronavirus coronavirus Coronavirus replication detected domain expressing found function in vitro Interaction Involvement mammalian mechanism molecular mechanisms multifunctional Nidovirale non-structural protein Nsp12 nsp14 Nsp3 platform Protein protein-protein interaction Proteins provided regulate reporter RNA genome SARS Coronavirus SARS-CoV sequence shown stimulated to screen Transcription unique Viral protein viruses [DOI] 10.1371/journal.pone.0003299 PMC 바로가기 [Article Type] Research Article
Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 ActivationResearch Article Published on 2008-06-132022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] Activation ADP-ribosylation Analysis ARF ARF1 association Author cells cellular cellular pathway characteristic complex connection coronavirus coronavirus Coronavirus replication Critical demonstrated dominant-negative downstream Endoplasmic reticulum Express expression Factors GBF1 GTPase GTPases guanine Host Human induce infected cell Infection inhibited inhibiting interfere involved knockdown MDCK cells membrane MHV Microscopy mouse mouse hepatitis mutant nucleotide observation provide Quantitative reduced Replication replication complex required RNA RNA virus secretory pathway significantly siRNA siRNAs structures target vesicles viruses [DOI] 10.1371/journal.ppat.1000088 PMC 바로가기 [Article Type] Research Article
SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epitheliumArticle Published on 2007-04-232022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] addition airway airway epithelial cells airway epithelium Antisera apical surface Atypical Calu-3 Calu-3 cell Cell cells cellular Characteristics ciliated ciliated cell consequence coronavirus coronavirus pathogenesis Coronavirus replication Culture cultures detected develop did not reduce dry cough efficient enzyme Epidemics epithelial epithelial cell epithelial cell line Experimental result feature Fever fluorescent Frame GFP hACE2 HAE Human human airway epithelia human airway epithelial Human airway epithelium human lung human pathogen in vitro in vitro model in vivo Infection inoculated insertion late stage log Lower respiratory tract lung lung epithelial mechanism Morphological new strain onset of symptoms ORF Pathogenesis pathology physiological Pneumonia Post-infection primary cell progeny virion Protein provide reached receptor reduced replicate replicated Replication reported reservoir respiratory virus resulting SARS Coronavirus SARS-CoV SARS-CoV GFP SARS-CoV infection Support susceptible target tight junction Trachea Vero E6 viral infection viral replication viral titer virus virus wild type zoonotic [DOI] 10.1016/j.virusres.2007.03.013 PMC 바로가기 [Article Type] Article