Saliva “Treat-and-Heat” Triplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for SARS-CoV-2SARS-CoV-2에 대한 타액 "치료 및 가열" 삼중 역전사 루프 매개 등온 증폭 분석Article Published on 2021-09-012022-09-11 Journal: Journal of biomolecular techniques : JBT [Category] SARS, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amplicon amplification Analysis clinically coronavirus cross-contamination detect diagnostic diagnostic testing diagnostics EDTA expected extraction extraction-free False positive faster Filipinos Fluorescence fraction identify infectious Infrastructure isothermal amplification Laboratory laboratory procedures Loop-mediated isothermal amplification majority melt curve. molecular molecular diagnostics nasopharyngeal Patient patients Phosphine predicted primer Primers proteinase Proteinase K protocol protocols provided qPCR test quantitative PCR respiratory reverse transcription RNA robustness RT-qPCR RT-qPCR test Saliva salivary SARS-CoV-2 severe acute respiratory syndrome Coronavirus triplex uracil Viral Viral load viral loads virus [DOI] 10.7171/jbt.21-3203-014 PMC 바로가기 [Article Type] Article
Factors Associated With Household Transmission of SARS-CoV-2 An Updated Systematic Review and Meta-analysisSARS-CoV-2의 가구 전파와 관련된 요인 업데이트된 체계적인 검토 및 메타 분석Meta-Analysis Published on 2021-08-272022-09-10 Journal: JAMA Network Open [Category] MERS, SARS, 변종, [키워드] 95% CI 95% CIs age Analysis Antibody test antibody tests applied article articles attack rate attack rates B.1.1.7 changes changes in close contact Comorbidities Comorbidity Contact contagious covariate covariates COVID-19 data extraction database diagnostic procedure diagnostic procedures eligibility eligible estimate Ethnicity extraction Factor Fever finding Follow-up guideline household household contact household contacts household infection household transmission Importance include increase in index case index cases Infection infection prevalence item language linear mixed model measure meta-analyses Meta-analysis number of household objective overlapped overlapping performed Point Population PRISMA Relevance reported Reporting representing Result resulting review SAR SAR estimate SARS-CoV-2 SARS-CoV-2 transmission secondary attack rate Selection Sex Source Study design study population Subgroup analyses symptom status synthesis systematic review systematic reviews tested transmissible variant Transmission Vaccine variant variants were assessed were excluded were used with infection Wuhan Wuhan, China [DOI] 10.1001/jamanetworkopen.2021.22240 PMC 바로가기 [Article Type] Meta-Analysis
Molecular Mirror Technology Facilitates High-Throughput, Accurate SARS-CoV-2 TestingMolecular Mirror 기술은 높은 처리량의 정확한 SARS-CoV-2 테스트를 용이하게 합니다.Research Article Published on 2021-08-252022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, [키워드] 95% CI acute SARS-CoV-2 infection analyzed Antigen approved Automation benefit can be used center Characteristics comparable confidence interval country COVID-19 COVID-19 pandemic Critical detect determine diagnose diagnostic diagnostic testing diagnostics Efficacy Emergency use authorization EUA evaluated extraction extraction-free method identify individual landscape limitation Mirror Molecular Mirror nasal NMR nuclear nuclear magnetic resonance nucleic acid nucleic acid amplification nucleic acid extraction offer PCR test PCR tests PCR-based assays positive proof proof of concept protocol Rapid Rapid tests reverse transcriptase reverse transcriptase PCR RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 nucleic acid SARS-CoV-2 testing sensitivity specimen subject Supply chain supply chains TaqPath technology Test Testing virus detection [DOI] 10.1128/Spectrum.00392-21 PMC 바로가기 [Article Type] Research Article
Evaluation of Self-Collected Midturbinate Nasal Swabs and Saliva for Detection of SARS-CoV-2 RNASARS-CoV-2 RNA 검출을 위한 자가 수집 중비갑개 비강 면봉 및 타액 평가Article Published on 2021-08-182022-09-11 Journal: Journal of Clinical Microbiology [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 authorization Care Consensus coronavirus COVID-19 COVID-19 pandemic detection diagnostic diagnostic testing Emergency Emergency use authorization exhibited extraction FDA Health Health care Heating individual investigated Laboratory midturbinate NAAT nasal swab nasopharyngeal NP swab NP swabs patients PCR assay positive proteinase Proteinase K Rapid reduced routine monitoring Saliva saliva specimen saliva specimens SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 RNA sensitivity severe acute respiratory syndrome Coronavirus specificity Supply shortages Swab swabs These data [DOI] 10.1128/JCM.00848-21 PMC 바로가기 [Article Type] Article
Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection programTrizol을 사용한 SARS-COV-2 진단을위한 대체 타액 검사 개발 : 대규모 탐지 프로그램을 찾고있는 소득이 낮은 국가에 적응Research Article Published on 2021-08-182022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] 2019-nCoV added Asymptomatic buffer caused CDC comparable coronavirus costly country development Diagnosis diagnosis of SARS-CoV-2 extraction Families handling healthcare healthcare worker Healthcare workers healthy help infected patients Infection less Mortality Nasopharyngeal swab nasopharyngeal swabs Non-invasive not need Novel coronavirus NPs one patient Panel Patient patient with SARS-CoV-2 patients with SARS-CoV-2 performed Personal protective equipment positive quarantine real-time RT-PCR reduce RNA room temperature RT-PCR RT-qPCR Saliva saliva test SARS-CoV-2 SARS-CoV-2 RNA Schools shown storage temperature Trizol Viral viral inactivation virus [DOI] 10.1371/journal.pone.0255807 PMC 바로가기 [Article Type] Research Article
A Policy-Driven Approach to Secure Extraction of COVID-19 Data From Research PapersBig Data Published on 2021-08-122022-10-31 Journal: Frontiers in Big Data [Category] COVID-19, [키워드] Analysis can be used Care Community Compliance COVID COVID-19 COVID-19 disease data integrity dataset develop example extraction Health healthcare Healthcare provider help HIPAA HTML information knowledge Knowledge graph language media MOST office paper PDF plasma Policy privacy privacy policy PROTECT question Regulation released Research restrict right System UMLS urgency White House [DOI] 10.3389/fdata.2021.701966 PMC 바로가기 [Article Type] Big Data
Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum인간 혈청에서 favipiravir의 정량을 위한 민감하고 빠르고 간단한 LC-MS/MS 방법의 개발 및 검증Article Published on 2021-06-302022-09-11 Journal: Journal of chromatography. B, Analytical technolog [Category] 치료제, [키워드] applicability applied biological samples clinical trial Concentration coronavirus disease Coronavirus disease 2019 correlation coefficient COVID-19 COVID-19 patient develop development drug level electrospray ionization extraction Favipiravir Formic acid human serum inhibitor LC-MS less linear mass spectrometry methanol patients with COVID-19 performed Phenomenex pilot study plasma samples polymerase positive protein precipitation quantitation receiving reported robust simple tandem Tandem mass spectrometry Treatment validation validation study Viral RNA was performed was used water [DOI] 10.1016/j.jchromb.2021.122768 PMC 바로가기 [Article Type] Article
Evaluation of a High-Definition PCR Assay for the Detection of SARS-CoV-2 in Extracted and Nonextracted Respiratory Specimens Collected in Various Transport Media다양한 운송 매체에서 수집된 추출 및 비추출 호흡기 표본에서 SARS-CoV-2 검출을 위한 고화질 PCR 분석 평가Article Published on 2021-06-172022-09-11 Journal: American journal of clinical pathology [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 benefit Chain Reaction collected conducted coronavirus Cycle threshold values demonstrated detection Extracted extraction extraction-free method Extraction-free PCR High-definition PCR increase in individual Limits of detection medium nucleic acid nucleic acid extraction PCR polymerase chain polymerase chain reaction positive protocol reagent reagents Real-time PCR reduced remained replicate respiratory RT-PCR SARS-CoV-2 severe acute respiratory syndrome Coronavirus specimen specimens swab specimen symptomatic and asymptomatic Transport Transport media transport medium upper respiratory [DOI] 10.1093/ajcp/aqab060 PMC 바로가기 [Article Type] Article
Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA ExtractionResearch Article Published on 2021-06-082022-10-27 Journal: BioMed Research International [Category] COVID-19, [키워드] alcohol analysed assessment average checked Concentration Council COVID-19 determinant E gene E-gene evaluate extracted RNA extraction group ICMR Isopropyl molecular Nanodrop parameter pool positive protocol purification purified purified RNA qRT-PCR reagents real-time RT-PCR Research RNA RNA purification RNAseP RT-PCR SARS-CoV-2 Screening second set selected sensitivity Sensitivity and specificity significantly increased specificity specimen specimens spectrophotometer step suggested treated Treatment was tested [DOI] 10.1155/2021/6653950 PMC 바로가기 [Article Type] Research Article
Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA ExtractionRNA 추출에서 이소 프로필 알코올 정제 단계를 이용한 풀 스크리닝에서 SARS-COV-2 분석의 성공적인 QRT-PCR 평가Research Article Published on 2021-06-082022-09-10 Journal: BioMed Research International [Category] 바이오마커, [키워드] alcohol analysed assessment average average value best checked Concentration Council COVID-19 determinant E gene E-gene evaluate extracted RNA extraction followed by group ICMR Isopropyl isopropyl alcohol Medical research molecular Nanodrop parameter pool positive protocol purification purified purified RNA purity qRT-PCR reagents real-time RT-PCR reason reason for Research RNA RNA purification RNAseP RT-PCR SARS-CoV-2 Screening second set selected sensitivity Sensitivity and specificity significantly increased specificity specimen specimens spectrophotometer step suggested treated Treatment was tested [DOI] 10.1155/2021/6653950 PMC 바로가기 [Article Type] Research Article