Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium1차 인간 기도 상피에서 SARS-CoV-2 향성의 단일 세포 분해능, 항바이러스 반응 및 요법에 대한 감수성Research Article Published on 2021-01-282022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, 치료제, [키워드] airway epithelial cells airway epithelium Antiviral antiviral immunity antiviral responses antiviral therapies antiviral therapy array blocked Cell cell population cell type cell types cellular ciliated cell ciliated cells Ciliated epithelial cells contribute COVID-19 pathogenesis dominant element elements epithelial cell epithelial cell type epithelial cell types epithelial cells expressed Factor flow cytometry gene signatures heterogeneity heterogenous host protease host protease TMPRSS2 Human airway epithelium hyperinflammatory responses IL-6 induction infect infected cell infected cells Infection inhibited initial interferon interferon response ISG ISGs mediator outcome Pathogenesis predominant primary human airway Remdesivir required respiratory respiratory tract RNA-sequencing SARS-CoV-2 SARS-COV-2 infection secretory cell secretory cells Single Cell susceptibility susceptible therapy These cells TMPRSS2 Treatment Treatment response variety Viral viral replication viral tropism [DOI] 10.1371/journal.ppat.1009292 PMC 바로가기 [Article Type] Research Article
Novel Targets of SARS-CoV-2 Spike Protein in Human Fetal Brain Development Suggest Early Pregnancy VulnerabilityNeuroscience Published on 2021-01-212022-10-31 Journal: Frontiers in Neuroscience [Category] COVID-19, [키워드] abundantly expressed ACE2 acute respiratory syndrome Analysis analyzed astrocytes ATP1A1 Brain brain development BrainSpan cardiovascular system cellular component co-expression coronavirus coronavirus disease Coronaviruses COVID-19 datasets development embryos expresse expressed expression facilitate fetal fetal brain Gene Expression GOLGA7 greater host cell membrane Human Immunity in viral indicate median Neonate Neurological complication Neuron normalized novel organ Organoid Paris Pathogenesis Placenta Pregnancy pregnant Protein question Region reported risk of infection RNA sequencing S protein SARS-CoV-2 scRNA seq Single Cell spike Spike protein strained target TMPRSS 2 TMPRSS2 vertical transmission virus vulnerability women ZDHHC5 [DOI] 10.3389/fnins.2020.614680 PMC 바로가기 [Article Type] Neuroscience
Rapid single cell evaluation of human disease and disorder targets using REVEAL: SingleCell™Database Published on 2021-01-062022-10-28 Journal: BMC Genomics [Category] COVID-19, [키워드] ACE2 Array native database Atla caused Cell cell type annotation cells cellular co-expression complex coronavirus COVID-19 COVID-19 pandemic Data storage and retrieval database dataset datasets described develop diagnostics disorder evaluate evaluated Evaluating exacerbated example explain expressed expressing expression expression profile facilitate Gene Expression Genes Hierarchical Human human cell human disease hypothese hypotheses include individual cell infected patient Infection infections Infectious disease Information extraction involved less manuscript Metadata Multiple studies oncology Ontology organs platform populations Precision prevalent Protein question Rapid resource returned SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 research SARS-CoV-2 virus SciDB selected Sequencing Single Cell Single cell analysis single-cell single-cell dataset spread of COVID-19 supplementary material target target gene targets threshold tissue TMPRSS2 utility Vaccine viral entry virulence virus worldwide pandemic [DOI] 10.1186/s12864-020-07300-8 PMC 바로가기 [Article Type] Database
Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface표면 성인 인간 결막, 윤부 및 각막 상피에서 SARS-CoV-2 진입 유전자의 동시 발현은 안구 표면을 통한 추가 진입 경로를 제안합니다Article Published on 2021-01-012022-09-10 Journal: The Ocular Surface [Category] SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] ACE2 ACE2 and TMPRSS2 expression ACE2 receptor Analysis Cell chromatin co-expression Conjunctiva conjunctival Cornea Corneal dataset embryonic ENhance epithelium expression pattern facilitate Factor fetal IFNg Immunohistochemical analysis Infection infection rate Inflammatory investigated loci molecular mechanism molecular mechanisms Multiple studies multiple study Ocular surface portal pro-inflammatory signal Propagation protease TMPRSS2 provide Result RNA SARS-CoV-2 SARS-CoV-2 entry Single Cell single-cell TMPRSS2 TMPRSS2 expression TNF transcriptional transmission route upregulation upstream Viral viral entry [DOI] 10.1016/j.jtos.2020.05.013 PMC 바로가기 [Article Type] Article
Targeting TMPRSS2 and Cathepsin B/L together may be synergistic against SARS-CoV-2 infectionTMPRSS2와 카텝신 B/L을 함께 표적으로 삼는 것은 SARS-CoV-2 감염에 대해 상승적일 수 있습니다Research Article Published on 2020-12-082022-08-13 Journal: PLoS Computational Biology [Category] Biochemistry, COVID-19, SARS, [키워드] ‘repurposing’ drugs achievement activate Activation blockade blocking blocking virus entry cathepsin Cathepsin B/L Cell cell lines cell population levels cell types cells clinical trials combination therapies combination therapy corresponding protease expression level COVID-19 COVID-19 pandemic cysteine cysteine protease cysteine proteases deployability dose-dependent manner drug drug combination drug combinations drugs targeting Effect Efficacy Efficiency effort entry of SARS-CoV-2 Evidence expression level facilitate help highlighting host protease host proteases host serine protease IMPROVE in vitro in vitro data in vivo independent independent entry pathway inhibitor inhibitors involved mathematical mathematical model minimal toxicities pathway Pathways potential treatment option predict predicted Prevent protease Proteases required SARS-CoV entry SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection Serine Single Cell Spike protein synergistic Synergistic drug combination Synergistic drug combinations synergy target cell target cells targeting the cell tissue tropism TMPRSS2 toxicities Treatment triggered virus entry [DOI] 10.1371/journal.pcbi.1008461 PMC 바로가기 [Article Type] Research Article
Direct Exposure to SARS-CoV-2 and Cigarette Smoke Increases Infection Severity and Alters the Stem Cell-Derived Airway Repair ResponseSARS-CoV-2 및 담배 연기에 직접 노출되면 감염 심각도가 증가하고 줄기 세포 유도 기도 복구 반응이 변경됩니다Article Published on 2020-12-032022-09-11 Journal: Cell Stem Cell [Category] Fulltext, MERS, 유전자 메커니즘, [키워드] abrogated Affect Air-liquid interface airway airway basal stem cells ALI culture ALI cultures basal stem cell basal stem cells Cell cigarette cigarette smoke COVID-19 Culture cultures current Direct disease disease spread epithelial exposure to implication increase increase in increased risk infected airway cells Infection Injury injury and repair innate immune response interferon interferon response lack Mucociliary clearance number of infected potential mechanism proliferation reducing repair response RNA SARS-CoV-2 severe COVID-19 severity short term Single Cell single cell RNA sequencing smoke smoking STEM stem cells Viral viral infection was reduced with infection [DOI] 10.1016/j.stem.2020.11.010 PMC 바로가기 [Article Type] Article
Tissue distributions of antiviral drugs affect their capabilities of reducing viral loads in COVID-19 treatmentResearch article Published on 2020-12-012022-10-05 Journal: European journal of pharmacology [Category] SARS, 신약개발, 유전자 메커니즘, 임상, 치료법, 치료제, [키워드] ACE2 acute respiratory syndrome Affect Analysis antiviral drug antiviral drugs Antiviral effect approved benefit Chloroquine clinical trials collected coronavirus Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients distribution distributions drug enzyme evaluate expression Favipiravir GI Tract Hydroxychloroquine Hypothesis inhibit inhibit SARS-CoV-2 intestine lung lung tissue patients profile profiles reduce reducing Remdesivir reported repurposing respiratory tract ribavirin RNA RNA sequencing SARS-CoV-2 Single Cell Stool tested Tissue distribution TMPRSS2 transmembrane treat Treatment umifenovir Viral load viral loads viral replication Viral RNA [DOI] 10.1016/j.ejphar.2020.173634 [Article Type] Research article
Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in AdultsArticle Published on 2020-11-042023-06-03 Journal: Frontiers in Immunology [Category] B형 간염, Fulltext, [키워드] bio-informatic Gene Expression immunimonitoring Lymph node multi-omic Single Cell Vaccine [DOI] 10.3389/fimmu.2020.580373 PMC 바로가기
On the whereabouts of SARS-CoV-2 in the human body: A systematic review인체 내 SARS-CoV-2의 행방: 체계적인 검토Research Article Published on 2020-10-302022-08-13 Journal: PLoS Pathogens [Category] Biology and Life Sciences, COVID-19, MERS, SARS, [키워드] ACE2 ACE2 entry-receptor ACE2 receptor addition aligned Blood body fluid body fluids Brain breast milk caused cerebrospinal fluid complex interplay component concerning consequence Containment correlated COVID-19 patients detect infectious virus Diagnosis distribution entry receptor Evidence expressed expression data expression level expression levels focus foundation heart highlight Host human body human host human population human tissue human tissues Impact implicates the presence improve diagnostics Infectious virus information intestines kidneys knowledge lung Lungs male genital male genitals Mucus multiple organ multiple organs multiple tissue multiple tissues observé organotropism of SARS-CoV-2 pandemic pandemic virus performed pharynx Proteins provide researcher RNA Saliva SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 entry receptor SARS-CoV-2 pathogenesis SARS-CoV-2 patients Scientific community Semen Single Cell Spread Stool systematic review These data Trachea Treatment treatment of COVID-19 patients Urine vessels Viral viral presence viral shedding viral shedding in body fluids virus [DOI] 10.1371/journal.ppat.1009037 PMC 바로가기 [Article Type] Research Article
Single-Cell Transcriptome Analysis Highlights a Role for Neutrophils and Inflammatory Macrophages in the Pathogenesis of Severe COVID-19Article Published on 2020-10-292022-10-31 Journal: Cells [Category] COVID-19, MERS, SARS, [키워드] activated Algorithm Analysis APOC1 ApoE BAL BCL2A1 Bronchoalveolar lavage Bronchoalveolar lavage (BAL) C1QB canonical category CCL2 CCL3 CCL5 CCL8 CCR1 Cell cellular chemotactic chemotaxis circulation Clustering Cohort complex coronavirus disease correlation COVID-19 CXCL8 Cytokine storm dataset diagnostic disease severity downregulated EPSTI1 FABP4 FIVE FPR1 functional gene signature gene signatures hallmark healthy subject healthy subjects ICGS2 identify IFI27 IFI30 Ifi44 IFI44L IFI6 IFIT1 IFIT3 IFITM1 IFITM2 IFITM3 IFNg IL17 IL1A IL1B IL1RN immune Immune cell Inflammatory inflammatory macrophages Inflammatory response interstitial iterative KLF6 Macrophage MALAT1 Mild mononuclear cell MX1 myeloid cells NEAT1 neutrophil Neutrophils NFkB OAS1 OAS2 OAS3 OASL pathway Patient patients PBMCs Peripheral blood phagocytes phagocytosis PLSCR1 PRL reduction reflected role S100A8 SARS-CoV-2 SAT1 several gene severe COVID-19 severity Signaling Single Cell SPP1 STAT3 subset suppressed systemically TGM2 therapeutic interventions TLR7 TLR9 TNF TYMP UMAP upstream with COVID-19 [DOI] 10.3390/cells9112374 PMC 바로가기 [Article Type] Article